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The Segregation and Elimination of Radioand Fluorescent-Belled Marine Bacteria from the Haemolymph of the Shore Crab, Carcinus Maenas

Published online by Cambridge University Press:  11 May 2009

K. N. White  and
N. A. Ratcliffe
K. N. White
Affiliation:
Department of Zoology, University College of Swansea, Swansea, SA2 8PP
N. A. Ratcliffe
Affiliation:
Department of Zoology, University College of Swansea, Swansea, SA2 8PP
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Extract

The dynamics of clearance, segregation and elimination of a marine bacterium, Moraxella sp., by the shore crab, Carcinus maenas (L.) has been studied utilizing fluorescent and radiolabelling techniques. In addition to the gills, the hepatopancreas was a major site of bacterial accumulation with sequestration occurring within haemocyte clumps and groups of stationary cells in this organ. The heart, excretory organ and subcuticular tissues also incorporated bacteria, but to a lesser extent. By the first day post-injection, many of the segregated micro-organisms had been removed from the organs. This latter process was not due to the exodus of laden haemocytes or of intact cell clumps from the host but seemed to result from lytic action by the host blood cells. Little material arising from such bacterial/haemocytic interaction was, however, immediately excreted, and much was relocated in the general body tissues as well as the gill nephrocytes.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 62 , Issue 4 , November 1982 , pp. 819 - 833
Copyright
Copyright © Marine Biological Association of the United Kingdom 1982

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References

REFERENCES

Acton, R. T.Weinheimer, P. F. & Evans, E. E. 1969. A bactericidal system in the lobster Homarus americanus. Journal of Invertebrate Pathology, 13, 463–464.CrossRefGoogle ScholarPubMed
Ali, M. 1966. The Histology of the Gills of Carcinus maenas (L.) and Other Decapod Crustacea. Ph.D. Thesis, University of Newcastle-upon-Tyne.Google Scholar
Bayne, C. J. 1973. Molluscan internal defense mechanism: the fate of 14C-labelled bacteria in the land snail Helix pomatia (L.). Journal of Comparative Physiology, 86, 17–25.CrossRefGoogle Scholar
Brown, A. C, 1967. Elimination of foreign particles by the snail, Helix aspersa. Nature, London, 213, 1154–1155.CrossRefGoogle Scholar
Brown, A. C. & Brown, R. J. 1965. The fate of thorium dioxide injected into the pedal sinus of Bullia (Gastropoda: Prosobranchiata). Journal of Experimental Biology, 42, 509–520.CrossRefGoogle Scholar
Cheng, T. C.Thakur, A. S. & Rifkin, E. 1969. Phagocytosis as an internal defense mechanism in the Mollusca with an experimental study of the role of leucocytes in the removal of ink particles in Littorina scabra, Linn. In Proceedings of the Symposium on Mollusca, part 11, pp. 546–563. Bangalore, India: Bangalore Press.Google Scholar
Drach, P. 1939. Mue et cycle d'internue chez les Crustaces Decapodes. Annales de I'Institut oce'anographique, 19, 103–391.Google Scholar
Evans, E. E.Painter, B.Evans, M. L.Weinheimer, P. & Acton, R. T. 1968. An induced bactericidin in the spiny lobster, Panulirus argus. Proceedings of the Society of Experimental Biology and Medicine, 128, 394–398.CrossRefGoogle ScholarPubMed
Gagen, S. J. & Ratcliffe, N. A. 1976. Studies on the in vivo cellular reaction and fate of injected bacteria in Galleria mellonella and Pieris brassicae larvae. Journal of Invertebrate Pathology, 28, 17–24.CrossRefGoogle Scholar
Ghiretti-Magaldi, A.Milanesi, C. & Togon, G. 1977. Hemopoiesis in Crustacea Decapoda: origin and evolution of hemocytes and cyanocytes of Carcinus maenas. Cell Differentiation, 6, 167–186.CrossRefGoogle Scholar
Johnson, P. T. 1976. Bacterial infection in the blue crab Callinectes sapidus: course of infection and histopathology. Journal of Invertebrate Pathology, 28, 25–36.CrossRefGoogle Scholar
Lison, L. 1942. Recherches sur l'histophysiologie comparee de l'excretion chez les arthropods. Me'moires de VAcademie royale de me'decine de Belgique, 19, 1–107.Google Scholar
Mccumber, L. J. & Clem, L. W. 1977. Recognition of viruses and xenogeneic proteins by the blue crab, Callinectes sapidus. I. Clearance and organ concentration. Developmental and Comparative Immunology, 1, 5–14.CrossRefGoogle ScholarPubMed
Mckay, D. &Jenkin, C. R. 1970. Immunity in the invertebrates. The fate and distribution of bacteria in normal and immunized crayfish (Parachaeraps bicarinatus). Australian Journal of Experimental Biology and Medical Science, 48, 599–607.CrossRefGoogle ScholarPubMed
Merrill, D. P.Mongeon, S. A. & Fisher, S. 1979. Distribution of fluorescent latex particles following clearance from the haemolymph of the freshwater crayfish Orconectes virilis (Hagen). Journal of Comparative Physiology, 132, 363–368.CrossRefGoogle Scholar
Pauley, G. B.Krassner, S. M. & Chapman, F. A. 1971. Bacterial clearance in the California sea hare, Aplysia californica. Journal of Invertebrate Pathology, 18, 227–239.CrossRefGoogle ScholarPubMed
Ratcliffe, N. A. & Rowley, A. R. 1979. Role of haemocytes in defence against biological agents. In Insect Haemocytes: Development, Forms, Functions and Techniques (ed. Gupta, A. P.), PP 331–414. Cambridge University Press.Google Scholar
Ratcliffe, N. A. & Rowley, A. F. 1981. Invertebrate Blood Cells, 2 vols. Academic Press.Google Scholar
Reade, P. 1968. Phagocytosis in the invertebrates. Australian Journal of Experimental Biology and Medical Science, 46, 219–229.CrossRefGoogle ScholarPubMed
Reade, P. & Rjeade, E. 1972. Phagocytosis in invertebrates. II. The clearance of carbon particles by the clam, Tridacna maxima. Journal of the Reticuloendothelial Society, 12, 349–360.Google ScholarPubMed
Rogers, A. W. 1972. Techniques of Autoradiography. 338 pp. Amsterdam: Elsevier.Google Scholar
Sabatini, D. D.Bensch, K. G. & Barrnett, R. J. 1963. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzyme activity by aldehyde fixation. Journal of Cell Biology, 17, 19–58.CrossRefGoogle ScholarPubMed
Sigel, M. & Cohen, N. 1982. The Reticuolendothelial System: A Comprehensive Treatise, vol. in. New York: Plenum Press. (In the Press.)Google Scholar
Smith, V. J. & Ratcliffe, N. A. 1978. Host defence reactions of the shore crab, Carcinus maenas (L.), in vitro. Journal of the Marine Biological Association of the United Kingdom, 58, 367–379.CrossRefGoogle Scholar
Smith, V. J. & Ratcliffe, N. A. 1980a. Host defence reactions of the shore crab, Carcinus maenas (L.): clearance and distribution of injected test particles. Journal of the Marine Biological Association of the United Kingdom, 60, 89–102.CrossRefGoogle Scholar
Smith, V. J. & Ratcliffe, N. A. 1980b. Cellular defence reactions of the shore crab, Carcinus maenas: in vivo haemocytic and histopathological responses to injected particles. Journal of Invertebrate Pathology, 35, 65–74.CrossRefGoogle Scholar
Smith, V. J. & Ratcliffe, N. A. 1981. Pathological changes in the nephrocytes of the shore crab, Carcinus maenas, following injection of bacteria. Journal of Invertebrate Pathology, 38, 113–121.CrossRefGoogle Scholar
Stauber, L. A. 1950. The fate of India ink injected intracardially into the oyster, Ostrea virginica Gmelin. Biological Bulletin. Marine Biological Laboratory, Woods Hole, Mass., 98, 226–241.Google Scholar
Tripp, M. R. 1960. Mechanisms of removal of injected microorganisms from the American oyster, Crassostrea virginica (Gmelin). Biological Bulletin. Marine Biological Laboratory, Woods Hole, Mass., 119, 273–282.CrossRefGoogle Scholar
Tripp, M. R. 1961. The fate of foreign material experimentally injected into the snail Australorbis glabratus. Journal of Parasitology, 47, 745–751.CrossRefGoogle Scholar
Wang, C. H. & Willis, D. L. 1965. Radiotracer Methodology in Biological Science. 382 pp. New Jersey: Prentice-Hall.Google Scholar
White, K. N. & Ratcliffe, N. A. 1980. Crustacean internal defence mechanisms: clearance and distribution of injected bacteria by the shore crab Carcinus maenas (L.). In Aspects of Developmental and Comparative Immunology, vol. I (ed. Solomon, J.B.), pp. 153–158. Oxford: Pergamon Press.Google Scholar