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Reproductive effects of endocrine disrupting chemicals, bisphenol-A and 17β-oestradiol, on Cerastoderma edule from south-west England: field study and laboratory exposure

Published online by Cambridge University Press:  11 April 2016

Amy L. Lusher Open the ORCID record for Amy L. Lusher [Opens in a new window] ,
Nick Pope  and
Richard D. Handy
Amy L. Lusher*
Affiliation:
The Marine Biological Association of the United Kingdom, Citadel Hill, Plymouth PL1 2PB, UK
Nick Pope
Affiliation:
The Marine Biological Association of the United Kingdom, Citadel Hill, Plymouth PL1 2PB, UK
Richard D. Handy
Affiliation:
School of Biomedical and Biological Sciences, Plymouth University, Drake Circus, Plymouth PL4 8AA, UK
*
Correspondence should be addressed to:A. Lusher, The Marine Biological Association of the United Kingdom, Citadel Hill, Plymouth PL1 2PB, UK email: [email protected]
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Abstract

Endocrine disruption has rarely been reported in field populations of the edible cockle and the context with the general health of the shellfish is unclear. This study examined the reproductive state of two Cerastoderma edule populations over a 6-month period to assess their reproductive condition, the incidence of intersex and presence of parasitic infection. A further seven native sites from south-west England were examined during the peak reproductive season to identify the presence of intersex within the region. Laboratory exposures of organisms collected from field populations showed a significantly female-biased sex ratio compared with controls when exposed to the endocrine disrupting chemicals, bisphenol-A (nominal concentration: 0.1 µg L−1) and 17β-oestradiol (nominal concentration: 0.1 µg L−1), but none of the chemical exposures induced intersex. Intersex was revealed in seven out of the nine native populations of C. edule sampled at peak reproductive season. The highest incidence and most severe case of intersex were reported at Lower Anderton on the River Tamer which also had a significantly female-biased sex ratio. Additionally, the dominant trematode family was the Bucephalaidae. Parasitic infection influences the maturity of C. edule by lowering both mean gonad index and condition index. These results suggest that endocrine disrupting chemicals could be contributing factors towards the development of intersex in C. edule.

Keywords

Gametogenesismortalityparasitesoestrogen mimicsE2BPA
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 97 , Issue 2 , March 2017 , pp. 347 - 357
Copyright
Copyright © Marine Biological Association of the United Kingdom 2016 

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