Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-22T17:28:59.272Z Has data issue: false hasContentIssue false
  • English
  • Français

Random Amplified Polymorphic Dna (Rapd) and Intraspecific Variation in Oerstedia Dorsalis (Hoplonemertea, Nemertea)

Published online by Cambridge University Press:  11 May 2009

Per Sundberg  and
Susanne Andersson
Per Sundberg
Affiliation:
Department of Zoology, University of Goteborg, Medicinaregatan 18, S-413 90 Goteborg, Sweden
Susanne Andersson
Affiliation:
Department of Zoology, University of Goteborg, Medicinaregatan 18, S-413 90 Goteborg, Sweden
Get access Rights & Permissions [Opens in a new window]

Extract

INTRODUCTION

The littoral/sublittoral hoplonemertean (phylum Nemertea) species Oerstedia dorsalis (Abildgaard, 1806) is described as polymorphic in external characters (Örsted, 1844; Burger, 1895; Brunberg, 1964). Sundberg (1984) grouped specimens in three classes based on external morphology and asked, using multivariate morphometrics, if there was a consistent correlation between external and internal characters. Such a correlation would have indicated a restricted gene flow between the externally different morphs, and thus the possibility of a group of species instead of one, polymorphic, species.

One of the forms in Sundberg (1984) appeared to be different from the other two morphs but the morphometric analysis was inconclusive. Later, Sundberg & Janson (1988) established by enzyme electrophoresis that this form was genetically different and it was later described as Oerstedia striata (Sundberg, 1988). A problem both in Sundberg (1984), and Sundberg & Janson (1988) was that one of the groupings (class ‘C’ in both studies) of specimens subsumed a number of morphs, all speckled or dotted but to varying degrees. These morphs may in fact represent different species, which would have been concealed in the electrophoretic and morphometric analysis as merely increased intraspecific variation. One of the ‘C’ morphs in Sundberg (1984) and Sundberg & Janson (1988) is light brown with numerous small dark brown/black dorsal spots. Based on one specimen from Anglesey, North Wales, Gibson (1988) described Oerstedia (Paroerstedia) nigrimaculata which is externally similar to this variety. Envall & Sundberg (1993) could not find any differences in internal characters between the type specimen of O. nigrimaculata and the brown form of O. dorsalis but hesitated to conclude that O. nigrimaculata is a variety of O. dorsalis.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 75 , Issue 2 , May 1995 , pp. 483 - 490
Copyright
Copyright © Marine Biological Association of the United Kingdom 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Burger, O., 1895. Die Nemertinen des Golfes von Neapel und der angrenzenden Meeres-Abschnitte. Fauna und Flora des Golfes von Neapel und der Angrenzenden Meeresabschnitte. Berlin, 22, 1–714.Google Scholar
Brunberg, L., 1964. On the nemertean fauna of Danish waters. Ophelia, 1, 77–111.CrossRefGoogle Scholar
Bucci, G. & Menozzi, P., 1993. Segregation analysis of random amplified polymorphic DNA (RAPD) markers in Picea abies Karst. Molecular Ecology, 2, 227–232.CrossRefGoogle ScholarPubMed
Crossland, S., Coates, D., Grahame, J. & Mill, P.J., 1993. Use of random amplified polymorphic DNAs (RAPDs) in separating two sibling species of Littorina. Marine Ecology Progress Series, 96, 301–305.CrossRefGoogle Scholar
Dawson, I.K., Chalmers, K.J., Waugh, R. & Powell, W., 1993. Detection and analysis of genetic variation in Hordeum spontaneum populations from Israel using RAPD markers. Molecular Ecology, 2, 151–159.CrossRefGoogle ScholarPubMed
Envall, M. & Sundberg, P., 1993. Intraspecific variation in nemerteans (Nemertea): synonymization of the genera Paroerstedia and Oerstediella with Oerstedia. Journal of Zoology, 230, 293–318.CrossRefGoogle Scholar
Gibson, R., 1988. A new species of marine nemertean from Anglesey, North Wales, with a discussion of the genus Paroerstedia Friedrich, 1955. Journal of Natural History, 22, 733–745.CrossRefGoogle Scholar
Hadrys, H., Balick, M. & Schierwater, B., 1992. Application of random amplified polymorphic DNA (RAPD) in molecular ecology. Molecular Ecology, 1, 55–63.CrossRefGoogle ScholarPubMed
Hadrys, H., Schierwater, B., Dellaporta, S.L., Desalle, R. & Buss, L.W., 1993. Determination of paternity in dragonflies by random amplified polymorphic DNA fingerprinting. Molecular Ecology, 2, 79–87.CrossRefGoogle ScholarPubMed
Kaukas, A., Dias Neto, E., Simpson, A.J.G., Southgate, V.R. & Rollinson, D., 1994. A phylogenetic analysis of Schistostoma haematobium group species based on randomly amplified polymorphic DNA. International Journal for Parasitology, 24, 285–290.CrossRefGoogle Scholar
Oppen, M.J.H. Van, Diekmann, O.E., Wiencke, C., Stam, W.T. & Olsen, J.L., 1994. Tracking dispersal routes: phylogeography of the Arctic-Antarctic disjunct seaweed Acrosiphonia arcta (Chlorophyta). Journal ofPhycology, 30, 67–80.CrossRefGoogle Scholar
Örsted, A.S., 1844. Entwiirfeiner systematischen Eintheilung und speciellen Beschreibung der Plattwürmer, auf microscopische Untersuchungen gegründet, Copenhagen: Reitzel.Google Scholar
Sambrook, J., Fritsch, E.F. & Maniatis, T., 1989. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Laboratory Press.Google Scholar
Smith, J.J., Scott-Craig, J.S., Leadbetter, J.R., Bush, G.L., Roberts, D.L. & Fulbright, D.W., 1994. Characterization of random amplified polymorphic DNA (RAPD) products from Xanthomonas campestris and some comments on the use of RAPD products in phylogenetic analysis. Molecular Phylogenetics and Evolution, 3, 135–145.CrossRefGoogle ScholarPubMed
Sundberg, P., 1984. Multivariate analysis of polymorphism in the hoplonemertean Oerstedia dorsalis (Abildgaard, 1806). Journal of Experimental Marine Biology and Ecology, 78,1–22.CrossRefGoogle Scholar
Sundberg, P., 1988. A new monostiliferous hoplonemertean (Nemertea), Oerstedia striata sp.n., from the west coast of Sweden. Zoologica Scripta, 17, 135–139.CrossRefGoogle Scholar
Sundberg, P. & Janson, K., 1988. Polymorphism in Oerstedia dorsalis revisited. Electrophoretic evidence for a species complex. Hydrobiologia, 156, 93–98.CrossRefGoogle Scholar
Thorpe, J.P. & Solé-Cava, A.M., 1994. The use of allozyme electrophoresis in invertebrate system-atics. Zoologica Scripta, 23, 3–18.CrossRefGoogle Scholar
Tibayrenc, M., Neubauer, K., Barnabé, C., Guerrini, F., Skarecky, D. & Ayala, F.J., 1993. Genetic characterization of six parasitic protozoa: parity between random-primer DNA typing and multilocus enzyme electrophoresis. Proceedings of the National Academy of Sciences of the United States of America, 90, 1335–1339.CrossRefGoogle ScholarPubMed
Tingey, S.V. & Tufo, J.P. del, 1993. Genetic analysis with random amplified polymorphic DNA markers. Plant Physiology, 101, 349–352.CrossRefGoogle ScholarPubMed
Welsh, J. & McClelland, M., 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research, 18, 7213–7218.CrossRefGoogle ScholarPubMed
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. & Tingey, S.V., 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18, 6531–6535.CrossRefGoogle ScholarPubMed