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Preliminary studies on the lipid metabolism of Neomysis integer, Involving Labelled Feeding Experiments

Published online by Cambridge University Press:  11 May 2009

R. J. Morris ,
C. F. Ferguson  and
J. E. G. Raymont
R. J. Morris
Affiliation:
National Institute of Oceanography, Wormley, Godalming, Surrey
C. F. Ferguson
Affiliation:
Department of Oceanography, University of Southampton
J. E. G. Raymont
Affiliation:
Department of Oceanography, University of Southampton
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Extract

A series of controlled, labelled feeding experiments were carried out on Neomysis integer in order to investigate its lipid metabolism. The techniques of thin-layer chromatography, preparative column chromatography, analytical and preparative gas-liquid chromatography and low-background 14C counting were used to follow the incorporation of a 14Clabelled fraction from a range of diets into the animal's lipid. Neomysis integer was found to be capable of converting dietary starch or short-chain saturated fatty acids to longchain polyunsaturated fatty acids, which were then incorporated into the TG and PL fractions.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 53 , Issue 3 , August 1973 , pp. 657 - 664
Copyright
Copyright © Marine Biological Association of the United Kingdom 1973

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References

Cowey, C. B. & Sargent, J. R., 1972. Fish nutrition. Advances in Marine Biology, 10, 382–492.Google Scholar
Culkin, F. & Morris, R. J., 1969. The fatty acids of some marine crustaceans. Deep-Sea Research, 16, 109–16.Google Scholar
Culkin, F. & Morris, R. J., 1970. The fatty acids of some cephalopods. Deep-Sea Research, 17, 171–4.Google Scholar
Folch, J., Lees, M. & Sloane, Stanley G. H., 1956. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, 226 (a), 497–509.CrossRefGoogle Scholar
Klein, F. K. & Rapoport, H., 1970. A two-dimensional pipette for sample application in preparative thin-layer chromatography. Journal of Chromatography, 47, 505–6.CrossRefGoogle Scholar
Klenk, E. & Kramer, G., 1960, Über die Biogenese der C20 und C22 Leberpolyenfettsavren bei der Ratte. Hoppe-Seyler's Zeitschrift für physiologische Chemie, 230, 218–32.CrossRefGoogle Scholar
Mead, J. F. & Kayama, M., 1967. Lipid metabolism in fish. In Fish Oils (ed. Stansby, M. E.), pp. 289–99. Westport, Conn.: Avi Publishing Co.Google Scholar
Mead, J. F., Kayama, M. & Reiser, R., 1960. Biogenesis of polyunsaturated acid in fish. Journal of the American Oil Chemist's Society, 37, 438–40.CrossRefGoogle Scholar
Morris, R. J., 1971(a). Seasonal and environmental effects on the lipid composition of Neomysis integer. Journal of the Marine Biological Association of the United Kingdom, 51, 21–31.CrossRefGoogle Scholar
Morris, R. J., 1971(b). Variations in the fatty acid composition of oceanic euphausiids. Deep-Sea Research, 18, 525–9.Google Scholar
Morris, R. J., 1971 (c). Comparison of the composition of oceanic copepods from different depths. Comparative Biochemistry and Physiology, 40 B, 275–81.Google Scholar
Morris, R. J., 1973. The Lipids of Marine Zooplankton. Ph.D. Thesis, Southampton University.Google Scholar
Morrison, W. R. & Smith, L. M., 1964. Preparation of fatty acid methyl esters and dimethylacetates from lipids with boron fluoride-methanol. Journal of Lipid Research, 5, 600–8.CrossRefGoogle ScholarPubMed
Raymont, J. E. G., Austin, J. & Linford, E., 1968. Biochemical studies on marine zooplankton. V. The composition of the major biochemical fractions in Neomysis integer. Journal of the Marine Biological Association of the United Kingdom, 48, 735–60.CrossRefGoogle Scholar
Yu, T. C. & Sinnhuber, R. O., 1972. Effect of dietary linolenic acid and docosahexaenoic acid on growth and fatty acid composition of Rainbow Trout. Lipids, 7, 450–4.CrossRefGoogle ScholarPubMed