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Phorbol Ester-Induced Metamorphosis in the Parasitic Barnacle, Loxothylacus Panopaei

Published online by Cambridge University Press:  11 May 2009

Henrik Glenner  and
Birger Brodin
Henrik Glenner
Affiliation:
Institute of Zoology, University of Copenhagen, 15 Universitetsparken, DK-2100 Copenhagen, Denmark.
Birger Brodin
Affiliation:
Biochemical Department, August Krogh Institute, University of Copenhagen, 13 Universitetsparken, DK-2100 Copenhagen, Denmark
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Extract

In order to induce metamorphosis in cyprids of the parasitic barnacle Loxothylacus panopaei (Gissler) (Cirripedia: Rhizocephala: Kentrogonida), we tested a number of compounds (ZR-512, 20-hydroxyecdysone, 3-isobutyl-l-methylxanthine and phorbol-12,13- dibutyrate) all known to cause metamorphosis in thoracican barnacles. Only one agent, phorbol-12,13-dibutyrate, had an effect and induced cypris larvae to complete metamorphosis without previous attachment. This suggests that diacylglycerol-activated protein kinase C plays a role in the metamorphosis of kentrogonid rhizocephalans.

Rhizocephalans are highly specialized crustaceans adapted for parasitic life on decapods. The juvenile parasite passes through an internal growth stage, where a root-like trophic organ invades the tissue and haemocoelic fluid of the host (for detailed information on rhizocephalan life history, see Haeg, 1992). As in free-living barnacles, a cyprid larva is the settling stage, and in thoracicans a juvenile barnacle is formed underneath the carapace of the cyprid larva. However, the rhizocephalans develop a so-called kentrogon, which accomplishes the injection of the primordial parasite (Glenner & Høeg, 1995). The degree of specialization towards a specific host means that the cypris larvae of rhizocephalans are extremely selective in their choice of substrata. They will not settle unless a specific host or part of a host, e.g. the gills, is offered. For this reason, the study of settlement and metamorphosis of these species has met with great difficulties.

Type
Short Communications
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 77 , Issue 1 , February 1997 , pp. 261 - 264
Copyright
Copyright © Marine Biological Association of the United Kingdom 1997

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