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On the nutrition and metabolism of zooplankton XIII. Further studies of steroids in Calanus

Published online by Cambridge University Press:  11 May 2009

S. C. M. O'Hara ,
S. J. Gaskellf  and
E. D. S. Corner
S. C. M. O'Hara
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth
S. J. Gaskellf
Affiliation:
Tenovus Institute for Cancer Research, Cardiff
E. D. S. Corner
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth
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Extract

The presence in Calanus finmarchicus of the sex hormone oestradiol-17β has been confirmed by gas chromatography-mass spectrometry (GC-MS) using the trimethylsilyl and heptafluorobutyrate derivatives.

Levels of the sex hormone testosterone have been measured by radioimmunoassay in Calanus helgolandicus collected from Plymouth Sound, and in C. finmarchicus captured off the Norwegian coast. Concentrations in C. helgolandicus varied during the year from 150 to 626 fg/animal, accounting for 2.3 to 437 x 10-7% of the lipid content and 10.8 to 64.8 x 10-6%percnt; of the total sterol fraction. The mean level in Stage V C. finmarchicus, captured during spring, was 196 fg/animal.

Testosterone was identified in C. finmarchicus by GC-MS of the tert-butyldimethylsilyl derivative.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 59 , Issue 2 , May 1979 , pp. 331 - 340
Copyright
Copyright © Marine Biological Association of the United Kingdom 1979

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References

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