Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-22T17:09:20.044Z Has data issue: false hasContentIssue false
  • English
  • Français

On the nutrition and metabolism of zooplankton XII. Measurements by radioimmunoassay of the levels of a steroid in Calanus

Published online by Cambridge University Press:  11 May 2009

S. C. M. O'Hara ,
E. D. S. Corner  and
C. C. Kilvington
S. C. M. O'Hara
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth
E. D. S. Corner
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth
C. C. Kilvington
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth
Get access Rights & Permissions [Opens in a new window]

Extract

A steroid that binds with mammalian oestradiol-17β antibody has been detected in Calanus helgolandicus Claus, collected from Plymouth Sound, and in Calanus finmarchicus Gunnerus captured off the coast of Norway. The specificity of the antibody, together with the chromatographic behaviour of the steroid on Sephadex LH-20, supports the view that the compound is the sex hormone oestradiol-17β (E2). The antibody has been used in measuring the amounts of E2 in the animals by radioimmunoassay, a technique not previously employed in studies with zooplankton and therefore described in detail.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 58 , Issue 3 , August 1978 , pp. 597 - 605
Copyright
Copyright © Marine Biological Association of the United Kingdom 1978

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abraham, G. E. 1974. Radioimmunoassay of steroids in biological materials. Actaendocrinologica, supplement 183, 4–42.Google Scholar
Berdugo, V. Harris, R. P. & O'Hara, S. C. M. 1977. The effect of petroleum hydrocarbons on reproduction of an estuarine planktonic copepod in laboratory cultures. Marine Pollution Bulletin, 8, 138–143.CrossRefGoogle Scholar
Boyd, J. M. & Alper, C. 1967. Blood (Chemical composition - Vertebrates). In The Encyclopedia of Biochemistry (ed. R. J. Williams and E. M. Lansford, Jr), pp. 147–150. New York: Rein-hold Publishing Corporation.Google Scholar
Carr, B. R. Mikhail, G. & Flickinger, G. L. 1971. Column chromatography of steroids on Sephadex LH-20. Journal of Clinical Endocrinology and Metabolism, 33, 358–360.CrossRefGoogle ScholarPubMed
Corner, E. D. S. Harris, R. P. Kilvington, C. C. & O'Hara, S. C. M. 1976. Petroleum compounds in the marine food web: short-term experiments on the fate of naphthalene in Calanus. Journal of the Marine Biological Association of the United Kingdom, 56, 121–133.CrossRefGoogle Scholar
Corner, E. D. S. Kilvington, C. C. & O'Hara, S. C. M. 1973. Qualitative studies on the metabolism of naphthalene in Maia squinado (Herbst). Journal of the Marine Biological Association of the United Kingdom, 53, 819–832.CrossRefGoogle Scholar
Donahue, J. K. 1957. Chromatographic identification of lobster estrogen. Research Bulletin, Department of Sea and Shore Fisheries, State of Maine, no. 28.Google Scholar
England, B. G. Niswender, G. D. & Midgley, A. R. Jr, 1974. Radioimmunoassay of estradiol-17β without chromatography. Journal of Clinical Endocrinology and Metabolism, 38, 42–50.CrossRefGoogle ScholarPubMed
Gower, D. B. 1975. Properties and subcellular location of enzymes involved in steroidogenesis (and the role of cytochrome P-450). In Biochemistry of Steroid Hormones (ed. H. L. J. Makin), pp. 105–125. Oxford: Blackwell Scientific Publications.Google Scholar
Kellie, A. E. 1975. Methods of steroid analysis. II. Competitive binding. In Biochemistry of Steroid Hormones (ed. H. L. J. Makin), pp. 211–226. Oxford: Blackwell Scientific Publications.Google Scholar
Lee, R. F. 1975. Fate of petroleum hydrocarbons in marine zooplankton. In Proceedings of the 1975 Conference on Prevention and Control of Oil Pollution, San Francisco, California, March 25–27, pp. 549–553. Washington, D.C.: American Petroleum Institute.Google Scholar
Lee, R. F. Nevenzel, J. C. & Lewis, A. G. 1974. Lipid changes during life cycle of marine copepod, Euchaeta japonica Marukawa. Lipids, 9, 891–898.CrossRefGoogle Scholar
Lee, R. F. Nevenzel, J. C. & Paffenhofer, G.-A. 1971. Importance of wax esters and other lipids in the marine food chain: phytoplankton and copepods. Marine Biology, 9, 99–108.CrossRefGoogle Scholar
Lee, R. F. & Williams, P. M. 1974. Copepod ‘slick’ in the Northwest Pacific Ocean. Naturwissenschaften, 61, 505–506.CrossRefGoogle Scholar
Lisk, R. D. 1961. Estradiol-17β in the eggs of the American lobster, Homarus Americanus. Canadian Journal Of Biochemistry And Physiology, 39, 659–662.CrossRefGoogle ScholarPubMed
Mikhail, G. Wu, C. H. Ferin, M. & VandeWiele, R. L. 1970. Radioimmunoassay of estrone and estradiol. In Karolinska Symposia on Research Methods in Reproductive Endocrinology. 2nd Symposium, Geneva, March 23–25. Steroid Assay by Protein Binding (ed. E. Diszfalusy), pp. 347–358. Stockholm: The Reproductive Endocrinology Research Unit.Google Scholar
Murphy, B. E. P. 1971. ‘Sephadex’ column chromatography as an adjunct to competitive protein binding assays of steroids. Nature: New Biology, 232, 21–24.Google ScholarPubMed
Ott, F. S. Harris, R. P. & O'Hara, S. C. M. 1978. Acute and sub-lethal toxicity of naphthalene and three methylated derivatives to the estuarine copepod, Eurytemora affinis. Marine Environmental Research. (In the Press.)CrossRefGoogle Scholar
Singer, S. C. & Lee, R. F. 1977. Mixed function oxygenase activity in blue crab, Callinectes sapidus: tissue distribution and correlation with changes during molting and development. Biological Bulletin. Marine Biological Laboratory, Woods Hole, Mass., 153, 377–386.CrossRefGoogle Scholar
Strott, C. A. 1975. Use of plasma-binding proteins for steroid hormone assays. In Methods in Enzymology, vol. 36. Hormone Action, part A, Steroid Hormones (ed. B. W. O'Malley and J. G. Hardman), pp. 34–48. New York: Academic Press.Google Scholar
Teshima, S. 1972. Studies on the sterol metabolism in marine crustaceans. Memoirs of the Faculty of Fisheries, Kagoshima University, 21, 69–147.Google Scholar
Varanasi, U. & Malins, D. C. 1977. Metabolism of petroleum hydrocarbons: accumulation and transformation in marine organisms. In Effects of Petroleum on Arctic and Subarctic Marine Environments and Organisms, vol. 2.Biological Effects (ed. D. C. Malins). New York: Academic Press. (In the Press.)Google Scholar
Walters, J. M. Cain, R. B. Corner, E. D. S. & Higgins, I. J. 1978. Cell-free benzpyrene hydroxylase activity in marine zooplankton. Proceedings of the Society for General Microbiology, 5,68.Google Scholar