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On the Nutrition and metabolism of zooplankton IV. The forms of nitrogen excreted By Calanus

Published online by Cambridge University Press:  11 May 2009

E. D. S. Corner  and
B. S. Newell
E. D. S. Corner
Affiliation:
The Plymouth Laboratory
B. S. Newell
Affiliation:
C.S.I.R.O., Division of Fisheries and Oceanography, Cronulla, N.S.W., Australia
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Extract

A study has been made of the nitrogenous compounds excreted by Calanus helgolandicus (Claus) collected at Plymouth.

Most of the excreted nitrogen is in the form of ammonia which accounts for 60–100% (average 74.3%) of the total, and some of the remainder may be lost as urea. There is no evidence for the excretion of measurable amounts of amino acids.

Whether the animals are starved or fed they are primarily ammonotelic, and the quantity of ammonia produced at 10° C (3.33 μg/g. dry body wt/day) is not significantly changed when the animals are used at an abnormally high experimental density. This latter condition does, however, lead to the production of large quantities of additional nitrogenous substances that give a positive reaction with ninhydrin.

Introduction

The amounts of nitrogen excreted by zooplankton have been measured by several workers. Harris (1959) used the method of Riley (1953) to estimate the copious quantities of ammonia produced by animals (mainly Acartia tonsa and A. clausi) collected from Long Island Sound; Beers (1964), in laboratory experiments with the chaetognath Sagitta hispida, estimated the excreted ammonia by the procedure of Kruse & Mellon (1952); and Corner, Cowey & Marshall (1965) determined the ammonia excreted by Calanus helgolandicus and C. finmarchicus, using a ninhydrin technique described by Moore & Stein (1954). The methods employed by Harris and by Beers are specific for ammonia: that used by Corner et al. estimates nitrogenous substances (e.g. amino acids) in addition to ammonia, but certain tests were made which seemed to exclude the possibility that these substances contributed significantly to the nitrogen excreted by the animals.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 47 , Issue 1 , February 1967 , pp. 113 - 120
Copyright
Copyright © Marine Biological Association of the United Kingdom 1967

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References

Beers, J. R., 1964. Ammonia and inorganic phosphorus excretion by the planktonic chaetognath, Sagitta hispida Conant. J. Cons. perm. int. Explor. Mer, Vol. 29, pp. 123–9.CrossRefGoogle Scholar
Corner, E. D. S., Cowey, C. B. & Marshall, S. M., 1965. On the nutrition and metabolism of zooplankton. III. Nitrogen excretion by Calanus. J. mar. biol. Ass. U.K., Vol. 45, pp. 429–42.CrossRefGoogle Scholar
Cowey, C. B. & Corner, E. D. S., 1963. On the nutrition and metabolism of zoo-plankton. II. The relationship between the marine copepod Calanus helgolandicus and paniculate material in Plymouth sea water in terms of amino acid composition. J. mar. biol. Ass. U.K., Vol. 43, pp. 495–511.CrossRefGoogle Scholar
Harris, E., 1959. The nitrogen cycle in Long Island Sound. Bull. Bingham oceanogr. Coll., Vol. 17, pp. 31–65.Google Scholar
Johannes, R. E., 1964. Phosphorous excretion and body size in marine animals: microzooplankton and nutrient regeneration. Science, N. Y., Vol. 146, pp. 923–4.CrossRefGoogle ScholarPubMed
Johannes, R. E. & Webb, K. L., 1965. Release of dissolved amino acids by marine zooplankton. Science, N. Y., Vol. 150, pp. 76–7.CrossRefGoogle ScholarPubMed
Ketchum, B. H., 1962. Regeneration of nutrients by zooplankton. Rapp. P.-v. Réun. Cons. perm. int. Explor. Mer, Vol. 153, pp. 142–7.Google Scholar
Kruse, J. & Mellon, M. G., 1952. Colorimetric determination of free ammonia with a pyridine-pyrazolone reagent. J. mar. Res., Vol. 24, pp. 1098–1100.Google Scholar
Macfadyen, D. A., 1944. Determination of ammonia evolved from a-amino acids by ninhydrin. J. biol. Chetn., Vol. 153, pp. 507–13.CrossRefGoogle Scholar
Moore, S. & Stein, W. H., 1954. A modified ninhydrin method for the photometric, determination of amino acids and related compounds. J. biol. Chem., Vol. 211, pp. 907–13.CrossRefGoogle Scholar
Newell, B. S., Morgan, B. & Cundy, J. M., 1967. The determination of urea in sea water. (In the Press.)Google Scholar
Parry, G., 1960. Excretion. In The Physiology of Crustacea (ed. Waterman, T. H.), Vol. 1, pp. 341–66. New York: Academic Press.Google Scholar
Pope, C. G. & Stevens, M. F., 1939. The determination of amino-nitrogen using a copper method. Biochem. J., Vol. 33, pp. 1070–7.CrossRefGoogle ScholarPubMed
Prosser, C. L. & Brown, F. A. Jr., 1962. Comparative Animal Physiology, 2nd ed. 688 pp. Philadelphia: W. B. Saunders Co.CrossRefGoogle Scholar
Riley, J. P., 1953. The spectrophotometric determination of ammonia in natural waters with particular reference to sea-water. Analytica chim. acta, Vol. 9, PP. 575–89.CrossRefGoogle Scholar
Zeuthen, E., 1947. Body size and metabolic rate in the animal kingdom with special regard to the marine micro-fauna. C. r. Trav. Lab. Carlsberg, Sir. Chim., Vol. 26, pp. 17–161.Google Scholar