An investigation on inheritance in hybrids between the three English species of Echinus was carried out in the Marine Biological Laboratory, Plymouth, during 1909–1912 by C. Shearer, W. de Morgan, and H. M. Fuchs. In a paper published in the Phil. Trans. Royal Soo., Ser. B, Vol. CCIV., p. 255, the results of this work were described in detail. At the time of publication, E. miliaris had been raised from the egg to maturity in the laboratory, in the course of one year, and a second generation had been obtained from these individuals, but none of the hybrid urchins had as yet reached maturity. This year, however, some of the hybrids have become sexually mature, and from them a second hybrid generation has been raised.

The urchins which have formed ripe genital products are four individuals of the cross E. esculentus X E. acutus (referred to below as EA) derived from fertilizations made in 1912. The largest of these urchins now measures 6 cm. in diameter, exclusive of the spines. On May 11th, 1914, two of these hybrids laid eggs in the tank in which they were kept. Naturally these eggs could not be used for experimental purposes, since they were deposited in the sea water of the aquarium circulation, and therefore not under sterile conditions. On June 6th I induced three of the four to deposit genital products without cutting them open, under conditions which excluded the possible presence of foreign eggs or spermatozoa. It is hardly necessary to mention here that, as in all the previous work on Echinus hybrids, the fact of the complete absence of such sperm was made certain by controls of unfertilized eggs, none of which segmented.