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The nutrient status of algal cells in continuous culture

Published online by Cambridge University Press:  11 May 2009

M. R. Droop
M. R. Droop
Affiliation:
Scottish Marine Biological Association, Oban, Scotland
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Extract

Chemostats were used to study the relation between growth rate, the rate of nutrient uptake and internal and external nutrient concentrations of two nutrients simultaneously. (Monochrysis lutheri: phosphorus and vitamin B12.)

Growth rate and internal concentrations of both limiting and excess nutrients are related by simple rectangular hyperbolas.

Control was shown to follow a threshold rather than multiplicative pattern; that is, non-limiting nutrients exert no control at all over the pattern of growth. The limiting nutrient was the one that showed the smallest cell quota: subsistence quota ratio.

Monochrysis populations exhibited two modes of growth. ‘Slow adapted cells’ differed from ‘fast adapted cells’ in the values of the constants for the above relation.

Uptake of both limiting and non-limiting nutrients was found to be controlled by internal as well as external substrate concentrations. There was thus a limit to luxury consumption of one nutrient when growth was limited by another.

The mathematical model formulated for growth in a chemostat (equations (23)–(29)) allowed prediction of external and internal substrate concentrations and rates of uptake of two nutrients and of biomass, given only the input concentrations of the two nutrients and the dilution rate. This model should apply equally well to growth in batch cultures; its possible application to natural populations was discussed.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 54 , Issue 4 , November 1974 , pp. 825 - 855
Copyright
Copyright © Marine Biological Association of the United Kingdom 1974

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References

REFERENCE

Caperon, J., 1968. Population growth response of Isochrysis galbana to nitrate variation at limiting concentrations. Ecology, 49, 866–72.CrossRefGoogle Scholar
Caperon, J. & Meyer, J., 1972a. Nitrogen limited growth of marine phytoplankton. I. Changes in population characteristics with steady-state growth rate. Deep-Sea Research, 19, 601–18.Google Scholar
Caperon, J. & Meyer, J., 1972b. Nitrogen limited growth of marine phytoplankton. II. Uptake kinetics and their role in nutrient limited growth of phytoplankton. Deep-Sea Research, 19, 619–32.Google Scholar
Daisley, K. W., 1961. Gel filtration of sea water: separation of free and bound forms of vitamin B12. Nature, London, 191, 868–9.CrossRefGoogle Scholar
Droop, M. R., 1966. Vitamin B12 and marine ecology. III. An experiment with a chemostat. Journal of the Marine Biological Association of the United Kingdom, 46, 659–71.CrossRefGoogle Scholar
Droop, M. R., 1968. Vitamin B12 and marine ecology. IV. The kinetics of uptake, growth and inhibition in Monochrysis lutheri. Journal of the Marine Biological Association of the United Kingdom, 48, 689–733.CrossRefGoogle Scholar
Droop, M. R., 1970. Vitamin B12 and marine ecology. V. Continuous culture as an approach to nutritional kinetics. Helgoländer wissenschaftliche Meeresuntersuchungen, 20, 629–36.CrossRefGoogle Scholar
Droop, M. R., 1973a. Nutrient limitation in osmotrophic Protista. American Zoologist, 13, 209–14.CrossRefGoogle Scholar
Droop, M. R. 1973b. Some thoughts on nutrient limitation in algae. Journal of Phycology, 9, 264–72.CrossRefGoogle Scholar
Dugdale, R. C. & Macisaac, J., 1971. A computation model for the uptake of nitrate in the Peru upwelling region. Investigacion pesquera, 35, 299–308.Google Scholar
Eppley, R. W. & Renger, E. M., 1974. On the coupling between nitrogen uptake and growth in a marine diatom. Journal of Phycology, 10, 15–23.Google Scholar
Eppley, R. W., Rogers, J. N. & McCarthy, J. J., 1968. Half saturation constants for uptake of nitrate and ammonium by marine phytoplankton. Limnology and Oceanography, 14, 912–20.CrossRefGoogle Scholar
Eppley, R. W. & Strickland, J. D. H., 1968. Kinetics of marine phytoplankton growth. In: Advances in Microbiology of the Sea (eds Droop, M. R. and Ferguson Wood, E. J.), 1, 23–62. London: Academic Press.Google Scholar
Eppley, R. W. & Thomas, W. H., 1969. Comparison of half saturation constants for growth and nitrate uptake of marine phytoplankton. Journal of Phycology, 5, 375–9.CrossRefGoogle ScholarPubMed
Fuhs, G. W., 1969. Phosphorus content and rate of growth in the diatoms Cyclotella nana and Thalassiosira fluviatilis. Journal of Phycology, 5, 312–21.CrossRefGoogle ScholarPubMed
Kristensen, H. P. O., 1956. A vitamin B12 binding factor formed in culture of Euglena gracilis, var bacillaris. Acta physiologica scandinavica, 36, 8–13.CrossRefGoogle Scholar
Monod, J., 1942. Recherches sur la Croissance des Cultures Bactériennes, 210 pp. Paris: Hermann.Google Scholar
Müller, H., 1972. Wachstum und Phosphatbedarf von Nitzschia actinasteroides (Lemm.). V. Goor in statischer und homokontinuierlicher Kulture unter Phosphatlimitierung, Archiv für Hydrobiologie, 38, suppl., 399–484.Google Scholar
Paasche, E., 1973a. Silicon and the ecology of marine plankton diatoms. I. Thalassicsira pseudonana (Cyclotella nana) grown in a chemostat with silicate as limiting nutrient Marine Biology, 19, 117–26.CrossRefGoogle Scholar
Paasche, E., 1973b. Silicon and the ecology of marine plankton diatoms. II. Silicate uptake kinetics in five diatom species. Marine Biology, 19, 262–9.CrossRefGoogle Scholar
Tett, P., Cottrell, J. C., Trew, D. O. & WOOD, B. J. B., 1974. Phosphorus quota and the chlorophyll-carbon ratio in marine phytoplankton. Limnology and Oceanography (in the Press).Google Scholar
Thomas, W. H. & Dodson, A. N., 1968. Effects of phosphate concentration on cell division rates and yield of a tropical oceanic diatom. Biological Bulletin. Marine Biological Laboratory, Woods Hole, Mass., 134, 199–208.CrossRefGoogle ScholarPubMed
Wright, R. T. & Hobbie, J. E. 1966. Use of glucose and acetate by bacteria and algae in aquatic ecosystems. Ecology, 47, 447–64.CrossRefGoogle Scholar