Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-31T15:38:33.286Z Has data issue: false hasContentIssue false
  • English
  • Français

A Micro-Analytical Scheme for the Determination of Protein, Carbohydrate, Lipid and RNA Levels in Marine Invertebrate Larvae

Published online by Cambridge University Press:  11 May 2009

D. L. Holland  and
P. A. Gabbott
D. L. Holland
Affiliation:
N.E.R.C. Unit of Marine Invertebrate Biology, Marine Science Laboratories, Menai Bridge, Anglesey, North Wales
P. A. Gabbott
Affiliation:
N.E.R.C. Unit of Marine Invertebrate Biology, Marine Science Laboratories, Menai Bridge, Anglesey, North Wales
Get access Rights & Permissions [Opens in a new window]

Extract

A micro-analytical scheme for the determination of protein, total carbohydrate, freereducing substances, total lipid, phospholipid and RNA levels in marine invertebrate larvae is described and discussed. The scheme is designed for use with 1–2 mg dry weight of tissue. Values of 171·0, 15·03, 50·4 and 11·58 μg/mg dry weight of larvae (flesh + shell) are given for the levels of protein, total carbohydrate, total lipid and RNA respectively, in newly released larvae of Ostrea edulis L.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 51 , Issue 3 , August 1971 , pp. 659 - 668
Copyright
Copyright © Marine Biological Association of the United Kingdom 1971

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Anastiassiadis, P. A. & Common, R. H. 1968. Some aspects of the reliability of chemical analyses. Analyt. Biochem., Vol. 22, pp. 409–23.Google Scholar
Bligh, E. G. & Dyer, W. F. 1959. A rapid method of total lipid extraction and purification. Can.J. Biochem. Physiol., Vol. 37, pp. 911–17.Google Scholar
Davies, O. L. (Ed.), 1947. Statistical Methods in Research and Production with special reference to the Chemical Industry. London: Oliver and Boyd.Google Scholar
Folin, O. & Malmros, H. 1929. An improved form of Folin's micro method for blood sugar determinations. J. biol. Chem., Vol., 83, pp. 115–20.Google Scholar
Giese, A. C. 1967. Some methods for study of the biochemical constituents of marine invertebrates. Oceanogr. mar. Biol., Vol. 5, pp. 159–86.Google Scholar
Griswold, B. L.Humoller, F. L. & Mcintyre, A. R. 1951. Inorganic phosphates and phosphate esters in tissue extracts. Analyt. Chem., Vol. 23, pp. 192–4.CrossRefGoogle Scholar
Marsh, J. B. & Weinstein, D. B. 1966. Simple charring method for determination of lipids J. Lipid Res., Vol. 7, pp. 574–6.CrossRefGoogle ScholarPubMed
Millar, R. H. & Scott, J. M. 1967. The larvae of the oyster Ostrea edulis during starvation. J. mar. biol. Ass. U.K., Vol. 47, pp. 475–84.CrossRefGoogle Scholar
Mosenthal, H. O. & Barry, E. 1946. Evaluation of blood sugar tests: significance of the nonglucose reducing substances and the anterio-venous blood sugar difference. Am. J. dig. Dis., Vol. 13, pp. 160–7.CrossRefGoogle Scholar
Munro, H. N. & Fleck, A. 1966. The determination of nucleic acids. In Methods of Biochemical analysis, No. 14, pp. 113–76, Ed. D. Glick. New York and London.CrossRefGoogle Scholar
Paul, J. 1958. Determination of the major constituents of small amounts of tissue. Analyst, Vol. 83, pp. 37–42.CrossRefGoogle Scholar
Schmidt, G. & Thannhauser, S. J. 1945. A method for determination of desoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissues. J. biol. chem., Vol. 161, pp. 83–9.Google Scholar
Shibko, S.Koivistoinen, P.Tratnyek, C. A.Newhare, A. R. & Friedman, L. 1967. A method for sequential quantitative separation and determination of Protein, RNA, DNA, lipid and glycogen from a single rat liver homogenate or from a subcellular fraction. Analyt. Biochem., Vol. 19, pp. 514–28.Google Scholar
Van Handel, E. 1965. Estimation of glycogen in small amounts of tissue. Analyt. Biochem., Vol. 11, pp. 256–65.CrossRefGoogle ScholarPubMed
Walne, P. R. 1970. Present problems in the culture of the larvae of Ostrea edulis. Helgoländer wiss. Meeresunters., Bd. 20, pp. 514–25.CrossRefGoogle Scholar