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The Logic of Ecological Experiments: A Case History From Studies of the Distribution of Macro-Algae on Rocky Intertidal Shores

Published online by Cambridge University Press:  11 May 2009

A. J. Underwood
Affiliation:
Institute of Marine Ecology, Zoology Building, University of Sydney, NSW 2006, Australia

Extract

The various steps involved in experimental investigations of the causes of observed ecological patterns are examined. A sequence of different steps (observation, model, hypothesis, null hypothesis, test) is identifiable. The logical necessity for distinguishing and evaluating each of these steps is discussed. The potential usefulness of explicit recognition of the logical relationships between the various parts of the procedure is evaluated by reference to experimental studies to determine the causes of boundaries of vertical distribution of macro-algae in rocky intertidal habitats. Pitfalls of experimental procedures can sometimes be recognized as failures in the logical progress from one part to the next in the sequence of steps necessary in any study. Particularly important is the early recognition of the existence of many, potentially conflicting, but sometimes interactive, models to account for any observed pattern. Greater attention to the relation-ships between the components of the logical structure of an experimental study might aid in reduction of errors in the design, analysis and interpretation of ecological experimental data.

Type
Research Article
Copyright
Copyright © Marine Biological Association of the United Kingdom 1991

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References

REFERENCES

Andrew, N.L. & Mapstone, B.D., 1987. Sampling and the description of spatial pattern in marine ecology. Oceanography and Marine Biology, Annual Review, 25, 3990.Google Scholar
Andrew, N.L. & Underwood, A.J., 1989. Patterns of abundance of the sea urchin Centrostephanus rodgersii (Agassiz) on the central coast of New South Wales, Australia. Journal of Experimental Marine Biology and Ecology, 131, 6180.CrossRefGoogle Scholar
Ayling, A.M., 1981. The role of biological disturbance in temperate subtidal encrusting communi-ties. Ecology, 62, 830847.CrossRefGoogle Scholar
Birch, L.C., 1979. The effect of species of animals which share common resources on one another's distribution and abundance. Fortschritte der Zoologie, 25, 197221.Google ScholarPubMed
Branch, G.M. & Branch, M.L., 1980. Competition between Cellana tramoserica (Sowerby)(Gastropoda) and Patiriella exigua (Lamarck)(Asteroidea) and their influence on algal standing stocks. Journal of Experimental Marine Biology and Ecology, 48, 3549.CrossRefGoogle Scholar
Carnap, R., 1962. Logical Foundations of Probability. Chicago: University of Chicago Press.Google Scholar
Chalmers, A.F., 1979. What is This Thing Called Science Brisbane: Queensland University Press.Google Scholar
Chamberlin, T.C., 1965. The method of multiple working hypotheses. Science, New York, 148, 754759.CrossRefGoogle ScholarPubMed
Chapman, A.R.O., 1973. A critique of prevailing attitudes towards the control of seaweed zonation on the sea shore. Botanica Marina, 16, 8082.CrossRefGoogle Scholar
Chapman, M.G., 1986. Assessment of some controls in experimental transplants of intertidal gastropods. Journal of Experimental Marine Biology and Ecology, 103, 181201.CrossRefGoogle Scholar
Choat, J.H. & Schiel, D.R., 1982. Patterns of distribution and abundance of large brown algae and invertebrate herbivores in subtidal regions of northern New Zealand. Journal of Experimental Marine Biology and Ecology, 60, 129162.CrossRefGoogle Scholar
Cohen, J., 1977. Statistical Power Analysis for the Behavioural Sciences. New York: Academic Press.Google Scholar
Connell, J.H., 1961a. The influence of interspecific competition and other factors on the distribution of the barnacle Chthamalus stellatus. Ecology, 42, 710723.CrossRefGoogle Scholar
Connell, J.H., 1961b. Effects of competition, predationby Thais lapillus, and other factors on natural populations of the barnacle Balanus balanoides. Ecological Monographs, 31, 61104.CrossRefGoogle Scholar
Connell, J.H., 1983. On the prevalence and relative importance of interspecific competition: evidence from field experiments. American Naturalist, 122, 661696.CrossRefGoogle Scholar
Connor, E.F. & Simberloff, D., 1979. You can't falsify ecological hypotheses without data. Bulletin of the Ecological Society of America, 60, 154155.Google Scholar
Connor, E.F. & Simberloff, D. 1984. Neutral models of species’ co-occurrence patterns. In Ecological Communities: Conceptual Issues and the Evidence (ed. Strong, D.R. Jr, et al.), pp. 316331. New Jersey: Princeton University Press.Google Scholar
Connor, E.F. & Simberloff, D., 1986. Competition, scientific method and null models in ecology. American Scientist, 75, 155162.Google Scholar
Creese, R.G., 1980. An analysis of distribution and abundance of populations of the high-shore limpet Notoacmea petterdi (Tenison-Woods). Oecologia, 45, 252260.CrossRefGoogle ScholarPubMed
Creese, R.G. & Underwood, A.J., 1982. Analysis of inter- and intra-specific competition amongst limpets with different methods of feeding. Oecologia, 53, 337346.CrossRefGoogle ScholarPubMed
Dayton, P.K., 1971. Competition, disturbance and community organization: the provision and subsequent utilization of space in a rocky intertidal community. Ecological Monographs, 41, 351389.CrossRefGoogle Scholar
Dayton, P.K., 1973. Dispersion, dispersal, and persistence of the annual intertidal alga, Postelsia palmaeformis Ruprecht. Ecology, 54, 433438.CrossRefGoogle Scholar
Dayton, P.K. & Oliver, J.S. 1980. An evaluation of experimental analyses of population and community patterns in benthic marine environments. In Marine Benthic Dynamics (ed. Tenore, K.R. And Coull, B.C.), pp. 93120. Columbia: University of South Carolina Press.Google Scholar
Dethier, M.N. & Duggins, D.O., 1984. An ‘indirect commensalism’ between marine herbivores and the importance of competitive hierarchies. American Naturalist, 124, 205219.CrossRefGoogle Scholar
J.M., Diamond 1986. Overview: laboratory experiments, field experiments and natural experiments. In Community Ecology (ed. Diamond, J.M. and Case, T.J.), pp. 322. New York: Harper & Row.Google Scholar
Duggins, D.O., 1981. Interspecific facilitation in a guild of benthic marine herbivores. Oecologia, 48, 157163.CrossRefGoogle Scholar
Dungan, M.L., 1986. Three-way interactions: barnacles, limpets, and algae in a Sonoran desert rocky intertidal zone. American Naturalist, 127, 292316.CrossRefGoogle Scholar
Edson, M.M., Foin, T.C. & Knapp, C.M., 1981. ‘Emergent properties’ and ecological research. American Naturalist, 118, 593596.CrossRefGoogle Scholar
Fagerström, T., 1987. On theory, data and mathematics in ecology. Oikos, 50, 258261.CrossRefGoogle Scholar
Feyerabend, P.K., 1975. Against Method. London: New Left Books.Google Scholar
Fisher, R.A. & Wishart, J., 1930. The arrangement of field experiments and the statistical reduction of results. Technical Communications of the Imperial Bureau of Soil Science, 10, 123.Google Scholar
Fletcher, W.J., 1984. Intraspecific variation in the population dynamics and growth of the limpet, Cellana tramoserica. Oecologia, 63, 110121.CrossRefGoogle ScholarPubMed
Fletcher, W.J., 1987. Interactions among subtidal Australian sea urchins, gastropods and algae: effects of experimental removals. Ecological Monographs, 57, 89109.CrossRefGoogle Scholar
Fletcher, W.J. & Underwood, A.J., 1987. Interspecific competition among subtidal limpets: effect of substratum heterogeneity. Ecology, 68, 387400.CrossRefGoogle Scholar
Foster, M.S., 1982. Factors controlling the intertidal zonation of Iridaea flaccida (Rhodophyta). Journal of Phycology, 18, 285294.CrossRefGoogle Scholar
Foster, M.S., 1990. Organization of macroalgal assemblages in the Northeast Pacific: the assumption of homogeneity and the illusion of generality. Hydrobiologia, 192, 2133.CrossRefGoogle Scholar
Frank, P.W., 1965. The biodemography of an intertidal snail population. Ecology, 46, 831844.CrossRefGoogle Scholar
Hacking, I., 1983. Representing and Intervening. Cambridge: Cambridge University Press.CrossRefGoogle Scholar
Hanson, N.R., 1959. Patterns of Discovery. Cambridge: Cambridge University Press.CrossRefGoogle Scholar
Hatton, H., 1932. Quelques observations sur le peuplement en Fucus vesiculosus des surfaces rochenses denudées. Bulletins de la Eaboratoire Marine du Musee d'Histoire Naturelle, 9, 16.Google Scholar
Hatton, H., 1938. Essais de bionomie explicative sur quelques espéces intercotidales d'algues et d'animaux. Annales de l'Institut Oceanographie, Monaco, 17, 241348.Google Scholar
Hawkins, S.J. & Hartnoll, R.G., 1983. Grazing of intertidal algae by marine invertebrates. Annual Review of Oceanography and Marine Biology, 21, 195282.Google Scholar
Hawkins, S.J. & Hartnoll, R.G., 1985. Factors determining the upper limits of intertidal canopy-forming algae. Marine Ecology Progress Series, 20, 265271.CrossRefGoogle Scholar
Hay, C., 1979. Some factors affecting the upper limit of the southern bull kelp Durvillaea antarctica (Chamisso) Hariot on two New Zealand shores. Journal of the Royal Society of New Zealand, 9, 279289.CrossRefGoogle Scholar
Heath, O.V.S., 1970. Investigation by Experiment. London: Edward Arnold.Google Scholar
Hempel, C.G., 1965. Aspects of Scientific Explanation. New York: Free Press.Google Scholar
Hempel, C.G., 1966. Philosophy of Natural Science. New Jersey: Prentice-Hall.Google Scholar
Hilborn, R. & Stearns, S.C., 1982. On inference in ecology and evolutionary biology: the problem of multiple causes. Acta Biotheoretica, 31, 145164.CrossRefGoogle ScholarPubMed
Hocutt, M., 1979. The Elements of Logical Analysis and Inference. Cambridge: Winthrop.Google Scholar
Hume, D., 1779. Dialogues Concerning Natural Religion 1779. 2nd edition, 1947. London: Nelson.Google Scholar
Hurlbert, S.H., 1981. A gentle depilation of the niche: dicean resource sets in resource hyperspace. Evolutionary Theory, 5, 177184.Google Scholar
Hurlbert, S.H., 1984. Pseudoreplication and the design of ecological field experiments. Ecological Monographs, 54, 187211.CrossRefGoogle Scholar
Jackson, J.B.C., 1981. Interspecific competition and species’ distributions: the ghost of theories and data past. American Zoologist, 21, 889901.CrossRefGoogle Scholar
Jernakoff, P., 1983. Factors affecting the recruitment of algae in a midshore region dominated by barnacles. Journal of Experimental Marine Biology and Ecology, 67, 1731.CrossRefGoogle Scholar
Kennelly, S.J., 1983. An Experimental Approach To The Study Of Factors Affecting Algal Colonization In A Sublittoral Kelp Forest. Journal of Experimental Marine Biology and Ecology, 68, 257276.CrossRefGoogle Scholar
Koyre, A., 1968. Metaphysics and Measurement. London: Chapman & Hall.Google Scholar
Kuhn, T., 1970. The Structure of Scientific Revolutions. 2nd edition. Chicago: University of Chicago Press.Google Scholar
I., Lakatos 1974. Falsification and the methodology of scientific research programmes. In Criticism and the Growth of Knowledge (ed. Lakatos, I. and Musgrave, A.E.), pp. 9196. Cambridge: Cambridge University Press.Google Scholar
Lemmon, E.J., 1971. Beginning Logic. Surrey: Nelson.Google Scholar
Lively, C.M. & Raimondi, P.T., 1987. Desiccation, predation, and mussel-barnacle interactions in the northern Gulf of California. Oecologia, 74, 304309.CrossRefGoogle ScholarPubMed
Loehle, C. J. 1987. Hypothesis testing in ecology: psychological aspects and the importance of theory maturation. Quarterly Review of Biology, 62, 397409.CrossRefGoogle ScholarPubMed
Lubchenco, J., 1980. Algal zonation in the New England rocky intertidal community: an experi-mental analysis. Ecology, 61, 333344.CrossRefGoogle Scholar
Lubchenco, J. & Games, S.D., 1981. A unified approach to marine plant-herbivore interactions. I. Population and communities. Annual Review of Ecology and Systematics, 12, 405437.CrossRefGoogle Scholar
Maclulich, J.H., 1986. Experimental evaluation of methods for sampling and assaying intertidal epilithic microalgae. Marine Ecology Progress Series, 34, 275280.CrossRefGoogle Scholar
Mather, K., 1967. The Elements of Biometry. London: Methuen.Google Scholar
McGuinness, K.A. & Underwood, A.J., 1986. Habitat structure and the nature of communities on intertidal boulders. Journal of Experimental Marine Biology and Ecology, 104, 97123.CrossRefGoogle Scholar
Mclntosh, R.P., 1985. The Background of Ecology. Cambridge: Cambridge University Press.CrossRefGoogle Scholar
Mentis, M.T., 1988. Hypothetico-deductive and inductive approaches in ecology. Functional Ecology, 12, 15.Google Scholar
Murray, B.G., Jr, 1982. On the meaning of density-dependence. Oecologia, 53, 370373.CrossRefGoogle ScholarPubMed
Nagel, E., 1961. The Structure of Science. London: Harcourt, Brace, World.CrossRefGoogle Scholar
Paine, R.T., 1974. Intertidal community structure. Experimental studies on the relationship between a dominant competitor and its principal predator. Oecologia, 15, 93120.CrossRefGoogle ScholarPubMed
Paine, R.T., 1979. Disaster, catastrophe, and local persistence of the sea palm Postelsia palmaeformis. Science, New York, 205, 685687.CrossRefGoogle ScholarPubMed
Peterson, C.H. & Black, R., 1988. Density-dependent mortality caused by physical stress interacting with biotic history. American Naturalist, 131, 257270.CrossRefGoogle Scholar
Platt, J.R., 1964. Strong inference. Science, New York, 146, 347353.CrossRefGoogle ScholarPubMed
Popper, K.R., 1968. The Logic of Scientific Discovery. London: Hutchinson.Google Scholar
Popper, K.R., 1969. Conjectures and Refutations. London: Routledge & Kegan Paul.Google Scholar
Reed, D.C., 1990. The effects of variable settlement and early competition on patterns of kelp recruitment. Ecology, 71, 776787.CrossRefGoogle Scholar
Roughgarden, J., 1983. Competition and theory in community ecology. American Naturalist, 122, 583601.CrossRefGoogle Scholar
Russell, B., 1912. Problems of Philosophy. London: Willian & Norgate.Google Scholar
Schoener, T.W., 1983. Field experiments on intraspecific competition. American Naturalist, 122, 240285.CrossRefGoogle Scholar
Schonbeck, M. & Norton, T.A., 1978. Factors controlling the upper limits of fucoid algae on the shore. Journal of Experimental Marine Biology and Ecology, 31, 303313.CrossRefGoogle Scholar
Schonbeck, M.W. & Norton, T.A., 1980. Factors controlling the lower limits of fucoid algae on the shore. Journal of Experimental Marine Biology and Ecology, 43, 131150.CrossRefGoogle Scholar
Simberloff D., 1980. A succession of paradigms in ecology: essentialism, materialism and probabilism. In Conceptual Issues in Ecology (ed. Saarinen, E.), pp. 6399. Dordrecht: Reidel.Google Scholar
Toft, C.A. & Shea, P.J., 1983. Detecting community-wide patterns: estimating power strengthens statistical inference. American Naturalist, 122, 618625.CrossRefGoogle Scholar
Trusted, J., 1979. The Logic of Scientific Inference. London: Macmillan.CrossRefGoogle Scholar
Underwood, A.J., 1976. Food competition between age-classes in the intertidal neritacean Nerita atramentosa Reeve (Gastropoda: Prosobranchia). Journal of Experimental Marine Biology and Ecology, 23, 145154.CrossRefGoogle Scholar
Underwood, A.J., 1978a. A refutation of critical tidal levels as determinants of the structure of intertidal communities on British shores. Journal of Experimental Marine Biology and Ecology, 33, 261276.CrossRefGoogle Scholar
Underwood, A.J., 1978b. An experimental evaluation of competition between three species of intertidal prosobranch gastropods. Oecologia, 33, 185202.CrossRefGoogle ScholarPubMed
Underwood, A.J., 1980. The effects of grazing by gastropods and physical factors on the upper limits of distribution of intertidal macroalgae. Oecologia, 46, 201213.CrossRefGoogle ScholarPubMed
Underwood, A.J., 1981a. Techniques of analysis of variance in experimental marine biology and ecology. Oceanography and Marine Biology, Annual Review, 19, 513605.Google Scholar
Underwood, A.J., 1981b. Structure of a rocky intertidal community in New South Wales: patterns of vertical distribution and seasonal changes. Journal of Experimental Marine Biology and Ecology, 51, 5785.CrossRefGoogle Scholar
Underwood, A.J., 1984. Vertical and seasonal patterns in competition for microalgae between intertidal gastropods. Oecologia, 64, 211222.CrossRefGoogle ScholarPubMed
A.J., Underwood 1985. Physical factors and biological interactions: the necessity and nature of ecological experiments. In The Ecology of Rocky Coasts (ed. Moore, P.G. and Seed, R.), pp. 372390. London: Hodder & Stoughton.Google Scholar
A.J., Underwood 1986. The analysis of competition by field experiments. In Community Ecology: Pattern and Process (ed. Kikkawa, J. and Anderson, D.J.), pp. 240268. Melbourne: Blackwells.Google Scholar
Underwood, A.J. & Denley, E.J. 1984. Paradigms, explanations and generalizations in models for the structure of intertidal communities on rocky shores. In Ecological Communities: Conceptual Issues and the Evidence (ed. Strong, D.R., Jr, et al.), pp. 151180. New Jersey: Princeton University Press.CrossRefGoogle Scholar
Underwood, A.J., Denley, E.J. & Moran, M.J., 1983. Experimental analyses of the structure and dynamics of mid-shore rocky intertidal communities in New South Wales. Oecologia, 56, 202219.CrossRefGoogle ScholarPubMed
Underwood, A.J. & Jernakoff, P., 1981. Effects of interactions between algae and grazing gastropods on the structure of a low-shore intertidal algal community. Oecologia, 48, 221233.CrossRefGoogle ScholarPubMed
Underwood, A.J. & Jernakoff, P., 1984. The effects of tidal height, wave-exposure, seasonality and rock-pools on grazing and the distribution of intertidal macroalgae in New South Wales. Journal of Experimental Marine Biology and Ecology, 75, 7196.CrossRefGoogle Scholar
Whewell, W., 1847. The Philosophy of the Inductivist Sciences, Volume 2. London: Parker.Google Scholar
Whittaker, R.H., 1956. Vegetation of the Great Smoky mountains. Ecological Monographs, 26, 180.CrossRefGoogle Scholar
Winer, B.J., 1971. Statistical Principles in Experimental Design, 2nd Edition. Tokyo: McGraw-Hill Kogakusha.Google Scholar
Wolcott, T.G., 1973. Physiological ecology and intertidal zonation in limpets (Acmaea): a critical look at ‘limiting factors’. Biological Bulletin. Marine Biological Laboratory, Woods Hole, 145, 389422.CrossRefGoogle Scholar
Worster, D., 1977. Nature's Economy: the Roots of Ecology. San Francisco: Sierra Book Clubs.Google Scholar