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Intraskeletal Matrix Proteins in Echinodermata

Published online by Cambridge University Press:  11 May 2009

E. S. Polson ,
L. L. Robbins  and
J. Lawrence
E. S. Polson
Affiliation:
Department of Biology, University of South Florida, Tampa, FL 33620, USA
L. L. Robbins
Affiliation:
Department of Geology, University of South Florida, Tampa, FL 33620, USA
J. Lawrence
Affiliation:
Department of Biology, University of South Florida, Tampa, FL 33620, USA
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Extract

An intraskeletal soluble matrix protein of 83 kD was isolated from the calcareous skeletal elements of living specimens of two asteroid species, one echinoid species, and an early Pleistocene fossil specimen of an extant echinoid species. Characterization of this protein by sodium dodecyl sulphate - polyacrylamide gel electrophoresis, reverse phase liquid chromatography, and amino acid composition analysis confirms its similarity to matrix proteins from other phyla. It is composed of a relatively high percentage of the acidic amino acids especially aspartic acid. The 83 kD protein stains only faintly with silver stain and not at all with Coomassie blue, as is typical of matrix proteins. As this protein occurs in both living and fossil echinoids, its analysis may provide information about phylogenetic relations.

Type
Short Communications
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 73 , Issue 3 , August 1993 , pp. 727 - 730
Copyright
Copyright © Marine Biological Association of the United Kingdom 1993

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References

Andrews, J. T., Bowen, D. Q. & Kidson, C., 1979. Amino acid ratios and the correlation of raised beach deposits in south-west England and Wales. Nature, London, 281, 556–558.CrossRefGoogle Scholar
Benson, S., Sucov, H., Stephens, L., Davidson, E. & Wilt, F., 1987. A lineage-specific gene encoding a major matrix protein of the sea urchin embryo spicule. I. Authentication of the cloned gene and its developmental expression. Developmental Biology, 120, 499–506.CrossRefGoogle Scholar
Cantatore, P., 1992. Organization, structure and evolution of echinoderm mitochondrial DNA. In Echinoderm research 1991 (ed. Scalera-Liaci, L. and Canicatti, C.), pp. 21–27. Rotterdam: A. A. Balkema.Google Scholar
Donachy, J. E., Drake, B., & Sikes, C. S., 1992. Sequence and atomic-force microscopy analysis of a matrix protein from the shell of the oyster Crassostrea virginica. Marine Biology, 114, 423–428.CrossRefGoogle Scholar
Dubois, P. & Jangoux, M., 1985. The microstructure of the asteroid skeleton (Asterias rubens). In Echinodermata (ed. Keegan, B. F. and O'connor, B. D. S.), pp. 507–512. Rotterdam: A. A. Balkema.Google Scholar
Hyman, L. H., 1955. The invertebrates: Echinodermata, the coelomate bilateria. Vol. IV. New York: McGraw-Hill.Google Scholar
Laemmli, U. K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London, 227, 680–685.CrossRefGoogle ScholarPubMed
Lowenstein, J. M., 1980. Species-specific proteins in fossils. Naturzvissenschaften, Berlin, 67, 343–346.Google ScholarPubMed
Marshall, C. R., 1992. Character analysis and the integration of molecular and morphological data in an understanding of sand dollar phylogeny. Molecular Biology and Evolution, 9, 309–322.Google Scholar
Matsumoto, H., 1929. Outline of a classification of Echinodermata. Science Reports of the Tokhoko Imperial University. Series 2. Geology, 13, 27–33.Google Scholar
Matsumura, T., Hasegawa, M. & Shigei, M., 1979. Collagen biochemistry and the phylogeny of echinoderms. Comparative Biochemistry and Physiology, 62B, 101–105.Google Scholar
McKinney, M. L., McNamara, K. J., Carter, B. D. & Donovan, S. K., 1992. Evolution of Paleogene echinoids: a global and regional view. In Eocene - Oligocene climatic and biotic evolution, (ed. Prothero, D. and Bergrenn, ), pp. 349–367. Princeton: Princeton University Press.CrossRefGoogle Scholar
Muyzer, G., Sandberg, P., Knapen, M. H. J., Vermeer, C., Collins, M., & Westbroek, P., 1992. Preservation of the bone protein osteocalcin in dinosaurs. Geology, 20, 871–874.2.3.CO;2>CrossRefGoogle Scholar
Muyzer, G., Westbroek, P. & Wehmiller, J. F., 1988. Phylogenetic implications and diagenetic stability of macromolecules from Pleistocene and recent shells of Mercenaria mercenaria. Historical Biology, 1, 135–144.CrossRefGoogle Scholar
Paul, C. R. C. & Smith, A. B., 1988. Echinoderm phylogeny and evolutionary biology. Oxford: Clarendon Press.Google Scholar
Robbins, L. L. & Donachy, J. E., 1991. Mineral regulating proteins from fossil planktonic Foraminifera. In Surface reactive peptides and polymers: discovery and commercialization (ed. Sikes, C. S. and Wheeler, A. P.), pp. 139–148. Washington DC: American Chemical Society.CrossRefGoogle Scholar
Robbins, L. L. & Healy-Williams, N., 1991. Towards a classification of planktonic Foraminifera based on biochemical, geochemical and morphological criteria. Journal of Foraminiferal Research, 21, 159–167.CrossRefGoogle Scholar
Robbins, L. L., Muyzer, G. & Brew, K., in press. Macromolecules from living and fossil biominerals: implications for the establishment of molecular phylogenies. In Organic geochemistry. Vol. 6. Topics in geobiology series (ed. Engel, M. and Macko, S.). Plenum Press.Google Scholar
Ventkatesan, M. & Simpson, R. T., 1986. Isolation and characterization of spicule proteins from Strongylocentrotus purpuratus. Experimental Cell Research, 166, 259–264.CrossRefGoogle Scholar
Weiner, S. & Addadi, L., 1991. Acidic macromolecules of mineralized tissues: the controllers of crystal formation. Trends in Biochemical Sciences, 16, 252–256.CrossRefGoogle ScholarPubMed