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Impact of low pH/high pCO2 on the physiological response and fatty acid content in diatom Skeletonema pseudocostatum

Published online by Cambridge University Press:  21 November 2016

Bárbara G. Jacob ,
Peter von Dassow ,
Joe E. Salisbury ,
Jorge M. Navarro  and
Cristian A. Vargas
Bárbara G. Jacob*
Affiliation:
Department of Aquatic System, Aquatic Ecosystem Functioning Lab (LAFE), Faculty of Environmental Sciences & Environmental Sciences Center EULA Chile, Universidad de Concepción, Concepción 4070386, Chile
Peter von Dassow
Affiliation:
Instituto Milenio de Oceanografía (IMO), Universidad de Concepción, Concepción 4070386, Chile Departamento de Ecología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Avenida Bernardo O′Higgins 340, Santiago 8331150, Chile UMI 3614, Evolutionary Biology and Ecology of Algae, CNRS-UPMC Sorbonne Universités, PUCCh, UACH, Station Biologique de Roscoff, Roscoff 29682, France
Joe E. Salisbury
Affiliation:
Ocean Process Analysis Laboratory, University of New Hampshire, Durham, NH 03824, USA
Jorge M. Navarro
Affiliation:
Laboratorio Costero de Recursos Acuáticos de Calfuco, Facultad de Ciencias, Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Independencia 641, Valdivia 5110566, Chile Centro FONDAP de Investigación en Dinámica de Ecosistemas Marinos de Altas Latitudes (IDEAL), Universidad Austral de Chile, Valdivia, Chile
Cristian A. Vargas
Affiliation:
Department of Aquatic System, Aquatic Ecosystem Functioning Lab (LAFE), Faculty of Environmental Sciences & Environmental Sciences Center EULA Chile, Universidad de Concepción, Concepción 4070386, Chile Instituto Milenio de Oceanografía (IMO), Universidad de Concepción, Concepción 4070386, Chile Center for the Study of Multiple-Drivers on Marine Socio-Ecological Systems (MUSELS), Universidad de Concepción, Concepción, Chile
*
Correspondence should be addressed to: B.G. Jacob, Department of Aquatic System, Aquatic Ecosystem Functioning Lab (LAFE), Faculty of Environmental Sciences & Environmental Sciences Center EULA Chile, Universidad de Concepción, Concepción 4070386, Chile email: [email protected]
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Abstract

pCO2/pH perturbation experiments were carried out under two different pCO2 levels to evaluate effects of CO2-driven ocean acidification on semi-continuous cultures of the marine diatom Skeletonema pseudocostatum CSA48. Under higher pCO2/lowered pH conditions, our results showed that CO2-driven acidification had no significant impact on growth rate, chlorophyll-a, cellular abundance, gross photosynthesis, dark respiration, particulate organic carbon and particulate organic nitrogen between CO2-treatments, suggesting that S. pseudocostatum is adapted to tolerate changes of ~0.5 units of pH under high pCO2 conditions. However, dissolved organic carbon (DOC) concentration and DOC/POC ratio were significantly higher at high pCO2, indicating that a greater partitioning of organic carbon into the DOC pool was stimulated by high CO2/low pH conditions. Total fatty acids (FAs) were significantly higher under low pCO2 conditions. The composition of FAs changed from low to high pCO2, with an increase in the concentration of saturated and a reduction of monounsaturated FAs. Polyunsaturated FAs did not show significant differences between pCO2 treatments. Our results lead to the conclusion that the balance between negative or null effect on S. pseudocostatum ecophysiology upon low pH/high pCO2 conditions constitute an important factor to be considered in order to evaluate the global effect of rising atmospheric CO2 on primary productivity in coastal ocean. We found a significant decrease in total FAs, however no indications were found for a detrimental effect of ocean acidification on the nutritional quality in terms of essential fatty acids.

Keywords

Diatomlow pHhigh CO2fatty acidDOCphotosynthesiselemental composition
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 97 , Issue 2 , March 2017 , pp. 225 - 233
Copyright
Copyright © Marine Biological Association of the United Kingdom 2016 

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