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First molecular estimate of sex-ratio of southern right whale calves, Eubalaena australis, for Brazilian waters

Published online by Cambridge University Press:  27 May 2009

Larissa Rosa de Oliveira ,
Paulo Henrique Ott ,
Paulo A.C. Flores ,
Salvatore Siciliano ,
Raquel Santos de Almeida  and
Sandro L. Bonatto
Larissa Rosa de Oliveira*
Affiliation:
Grupo de Estudos de Mamíferos Aquáticos do Rio Grande do Sul (GEMARS), Rua Felipe Neri 382/203, Porto Alegre, RS, 90440-150, Brazil Centro de Estudos Costeiros, Limnológicos e Marinhos da Universidade Federal do Rio Grande do Sul (CECLIMAR/UFRGS), Avenida Tramandaí 976, Imbé, RS, 95625-000, Brazil Laboratório de Biologia Evolutiva e Conservação de Vertebrados, Universidade de São Paulo (LABEC-USP), Departamento de Biologia (Genética), Rua do Matão 277, São Paulo, SP, 05508-900, Brazil Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga 6681, Porto Alegre, RS, 90619-900, Brazil
Paulo Henrique Ott
Affiliation:
Grupo de Estudos de Mamíferos Aquáticos do Rio Grande do Sul (GEMARS), Rua Felipe Neri 382/203, Porto Alegre, RS, 90440-150, Brazil Centro de Estudos Costeiros, Limnológicos e Marinhos da Universidade Federal do Rio Grande do Sul (CECLIMAR/UFRGS), Avenida Tramandaí 976, Imbé, RS, 95625-000, Brazil Universidade Estadual do Rio Grande do Sul (UERGS), Avenida Mostardeiro 3635, Cidreira, RS, 95595-000, Brazil
Paulo A.C. Flores
Affiliation:
Centro Nacional de Pesquisa, Conservação e Manejo de Mamíferos Aquáticos – CMA, ICMBio CMA SC – Rod. Maurício Sirotsky Sobrinho, s/n, km02, Jurerê, Florianópolis, SC, 88053-700, Brazil
Salvatore Siciliano
Affiliation:
Escola Nacional de Saúde Pública/FIOCRUZ, Departamento de Endemias Samuel Pessoa, Rua Leopoldo Bulhões, 1480-60, ondar, sala 620, Rio de Janeiro, RJ, 21041-210, Brazil
Raquel Santos de Almeida
Affiliation:
Grupo de Estudos de Mamíferos Aquáticos do Rio Grande do Sul (GEMARS), Rua Felipe Neri 382/203, Porto Alegre, RS, 90440-150, Brazil Centro de Estudos Costeiros, Limnológicos e Marinhos da Universidade Federal do Rio Grande do Sul (CECLIMAR/UFRGS), Avenida Tramandaí 976, Imbé, RS, 95625-000, Brazil Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga 6681, Porto Alegre, RS, 90619-900, Brazil
Sandro L. Bonatto
Affiliation:
Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga 6681, Porto Alegre, RS, 90619-900, Brazil
*
Correspondence should be addressed to: L.R. de Oliveira, Grupo de Estudos de Mamíferos Aquáticos do Rio Grande do Sul (GEMARS), Rua Felipe Neri 382/203, Porto Alegre, RS, 90440-150, Brazil email: [email protected]
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Abstract

The southern right whale (Eubalaena australis) was one of the most intensively hunted whales between the 17th and 20th centuries in the southern hemisphere. Recent estimates indicate that today there are around 7000 whales, representing 5 to 10% of its original population. On the other hand, recent studies estimated that the population that migrates to the Brazilian coast grew by 14% from 1987 to 2003. However, there is no information about sex-ratio for adults or for calves in this region, which is an important parameter for understanding the biology of the species. We present here the first estimate of calves' sex-ratio of southern right whales found along the southern Brazilian coast, one of the most important wintering grounds for the species. Sex was molecularly indentified for 21 biopsies collected from calves between 1998 and 2002, along the coast of Rio Grande do Sul and Santa Catarina States, in southern Brazil. The sex-ratio was two females for one male, however, it was not statistically different (χ2 test, α = 0.05; df = 1) from the expected ratio of 1:1. This result is in accordance with the sex-ratio estimated for the species of all ages using external morphology (and behaviour information), as well as for most species of baleen whales.

Keywords

cetaceanscalfEubalaena australisgenetic sex identificationsex-ratiosouthern right whale
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 89 , Special Issue 5: Cetaceans , August 2009 , pp. 1003 - 1007
Copyright
Copyright © Marine Biological Association of the United Kingdom 2009

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References

REFERENCES

Amos, W. and Hoelzel, A.R. (1991) Long-term preservation of whale skin for DNA analysis. In Hoelzel, A.R. (ed) Genetic ecology of whales and dolphins. Report of the International Whaling Commission, Special Issue 13, 99–103.Google Scholar
Baker, C.S., Patenaude, N. J., Bannister, J.L., Robins, J and Kato, H. (1999) Distribution and diversity of mtDNA lineages among southern right whales (Eubalaena australis) from Australia and New Zealand. Marine Biology 134, 1–7.CrossRefGoogle Scholar
Bannister, J., Burnell, S., Burton, C. and Kato, H. (1997) Right whales off southern Australia: direct evidence for a link between onshore breeding grounds and offshore probable feeding grounds. Report of the International Whaling Commission 47, 441–444.Google Scholar
Bannister, J.L., Pastene, L.A. and Burnell, S.R. (1999) First record of movements of a southern right whale (Eubalaena australis) between warm water breeding grounds and the Antarctic Ocean, South of 60°S. Marine Mammal Science 15, 1337–1342.CrossRefGoogle Scholar
Bérubé, M., Aguilar, A., Dendanto, D., Larsen, F., Notarbartolo Di Sciara, G., Sears, R., Sigurjónsson, J., Urban, J. and Pasbøll, P.L. (1998) Population genetic structure of North Atlantic, Mediterranean Sea and Sea of Cortez fin whales, Balaenoptera physalus (Linnaeus 1758): analysis of mitochondrial and nuclear loci. Molecular Ecology 7, 585–599.CrossRefGoogle ScholarPubMed
Best, P.B., Payne, R., Rowntree, V., Palazzo, J.T. and Both, M.D.C. (1993) Long-range movements of South Atlantic Right whales Eubalaena australis. Marine Mammal Science 9, 227–234.CrossRefGoogle Scholar
Brown, M.W., Helbig, R., Boag, P.T., Gaskin, D.E. and White, B.N. (1991a) Sexing beluga whales (Delphinapterus leucas) by means of DNA markers. Canadian Journal of Zoology 69, 1971–1976.CrossRefGoogle Scholar
Brown, M., Kraus, S.D. and Gaskin, D.E. (1991b) Reaction of North Atlantic right whales (Eubalaena glacialis) to skin biopsy sampling for genetic and pollutant analysis. In Hoelzel, A.R. (ed) Genetic ecology of whales and dolphins. Report of the International Whaling Commission, Special Issue 13, 81–89.Google Scholar
Brown, M.W., Kraus, S.D., Gaskin, D.E. and White, B.N. (1994) Sexual composition and analysis of reproductive females in the North Atlantic right whale, Eubalaena glacialis, population. Marine Mammal Science 10, 253–265.CrossRefGoogle Scholar
Castello, H.P. and Pinedo, M.C. (1979) Southern Right whales (Eubalaena australis) along the southern Brazilian coast. Journal of Mammalogy 60, 429–430.CrossRefGoogle Scholar
Câmara, I.G. and Palazzo, J.T. (1986) Novas informações sobre a presença de Eubalaena australis no sul do Brasil. In Abstracts of the ‘Primera reunión de trabajo de expertos en mamíferos acuáticos de America del Sur’. First workshop meeting of South American Aquatic Mammals Specialist Group, Buenos Aires, Argentina, October 1986, pp. 35–41.Google Scholar
Chivers, S.J. (2002) Cetacean life history. In Perrin, W.F., Wursig, B. and Thewissen, J.G.M. (eds) Encyclopedia of marine mammals. San Diego, CA: Academic Press, pp. 221–225.Google Scholar
Clapham, P.J., Berule, M. and Mattila, D.K. (1995) Sex-ratio of the Gulf of Maine humpback whale population. Marine Mammal Science 11, 227–231.CrossRefGoogle Scholar
Cunha, H. and Solé-Cava, A.M. (2007) Molecular sexing of tucuxi dolphins (Sotalia guianensis and Sotalia fluviatilis) using samples from biopsy darting and decomposed carcasses. Genetics and Molecular Biology 30, 1186–1188.CrossRefGoogle Scholar
Danilewicz, D. and Oliveira, L.R. (2003) Cetáceos. In Reis, R., Fontana, C. and Benke, G. (eds) Livro Vermelho da Fauna Ameaçada de Extinção do Rio Grande do Sul. Porto Alegre: Edipucrs, pp. 535–545.Google Scholar
Frankham, R., Ballou, J.D. and Briscoe, D.A. (2002) Introduction to conservation genetics. Cambridge: Cambridge University Press.CrossRefGoogle Scholar
Gompper, M.E., Gittleman, J.L. and Wayne, R.K. (1998) Dispersion, phylopatry and, genetic relatedness in a social carnivore: comparing males and females. Molecular Ecology 7, 157–163.CrossRefGoogle Scholar
Greig, A.B., Secchi, E.R., Zerbini, A.N. and Dalla Rosa, L. (2001) Stranding events of southern right whales, Eubalaena australis, in Southern Brazil. Journal of Cetacean Research and Management 2, 157–160.Google Scholar
Groch, K.R., Palazzo, J.T. Jr, Flores, P.A.C., Adler, F.R. and Fabian, M.E. (2005) Recent rapid increases in the right whale (Eubalaena australis) population off southern Brazil. The Latin American Journal of Aquatic Mammals 14, 41–48.Google Scholar
Hughes, C. (1998) Integrating molecular techniques with field methods in studies of social behaviour: a revolution results. Ecology 79, 383–399.CrossRefGoogle Scholar
IBAMA (2003) Lista da Fauna Brasileira Ameaçada de Extinção. Available via DIALOG. http://www.ibama.gov.br/fauna/extincao.htm. Accessed 14 March 2008.Google Scholar
IUCN (2007) 2007 IUCN Red List of Threatened Species. Available via DIALOG. http://www.redlist.org http://www.iucnredlist.org. Accessed 14 March 2008.Google Scholar
IWC (2001) Report of the workshop on the comprehensive assessment of the right whales: a worldwide comparison. Journal of Cetacean Research and Management 2, 1–60.Google Scholar
Knowlton, A.R.S., Kraus, D. and Kennedy, R.D. (1994) Reproduction of North Atlantic right whales (Eubalaena glacialis). Canadian Journal of Zoology 72, 1297–1305.CrossRefGoogle Scholar
Mills, L.S. and Smouse, P.E. (1994) Demographic consequences of inbreeding in remnant populations. American Naturalist 144, 412–431.CrossRefGoogle Scholar
Ott, P.H. (2002) Diversidade genética e estrutura populacional de duas espécies de cetáceos do Atlântico Sul Ocidental: Pontoporia blainvillei e Eubalaena australis. PhD thesis. Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.Google Scholar
Page, D.C., Mosher, R., Simpson, E.M., Fisher, E.M.C., Mardon, G., Pollack, J., McGillivray, B., de la Chapelle, A. and Brown, L.G. (1987) The sex-determining region of the human Y chromosome encodes a finger protein. Cell 51, 1091–1104.CrossRefGoogle ScholarPubMed
Palazzo, J.T. Jr and Carter, L.A. (1983) A caça as baleias no Brasil. Porto Alegre, Brazil: AGAPAN.Google Scholar
Pasbøll, P.J., Vader, A., Bakke, I. and El-Gewely, M.R. (1992) Gender determination in cetaceans by the polymerase chain reaction. Canadian Journal of Zoology 70, 2166–2170.CrossRefGoogle Scholar
Patenaude, N.J., Portway, V.A., Schaeff, C.M., Bannister, J.L., Best, P.B., Payne, R.S., Rowntree, V.J., Rivarola, M. and Baker, C.S. (2007) Mitochondrial DNA diversity and population structure among southern right whales (Eubalaena australis). Journal of Heredity 98, 147–157.CrossRefGoogle ScholarPubMed
Pomilla, C. and Rosenbaum, H.C. (2006) Estimates of relatedness in groups of humpback whales (Megaptera novaeangliae) on two wintering grounds of the Southern Hemisphere. Molecular Ecology 15, 2541–2555.CrossRefGoogle ScholarPubMed
Ralls, K., Brownell, R.L. Jr and Ballou, J. (1980) Differential mortality by sex and age in mammals, with specific reference to the sperm whale. Report of the International Whaling Commission, Special Issue 2, 233–243.Google Scholar
Roman, J. and Palumbi, S.R. (2003) Whales before whaling in the North Atlantic. Science 301, 508.CrossRefGoogle ScholarPubMed
Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.Google Scholar
Shaw, C., Wilson, P. and White, B.N. (2003) A reliable molecular method of gender determination for mammals. Journal of Mammalogy 84, 123–128.2.0.CO;2>CrossRefGoogle Scholar
Steeves, T.E., Darling, J.D., Rosel, P.E., Schaeff, C.M. and Fleischer, R.C. (2001) Preliminary analysis of mitochondrial DNA variation in a southern feeding group of eastern North Pacific gray whales. Conservation Genetics 2, 379–384.CrossRefGoogle Scholar
Tormosov, D.D, Mikhalev, Y.A., Best, P.B., Zemsky, V.A., Sekiguchi, K. and Brownell, R.L. Jr (1998) Soviet catches of southern right whales, Eubalaena australis, 1951–1971, biological data and conservation implications. Biological Conservation 86, 185–97.CrossRefGoogle Scholar
Townsend, C.H. (1935) The distribution of certain whales as shown by logbook records of American whaleships. Zoologica 19, 1–50.Google Scholar
Valsecchi, E., Hale, P., Corkeron, P. and Amos, W. (2002) Social structure in migrating humpback whales (Megaptera novaeangliae). Molecular Ecology 11, 507–518.CrossRefGoogle ScholarPubMed
Weller, D.W., Bradford, A.L., Lang, A.R., Kim, H.W., Sidorenko, M., Tsidulko, G.A, Burdin, A.M. and Brownell, R.L.Jr (2008) Status of western gray whales off northeastern Sakhalin Island, Russia. Report of the International Whaling Commission SC/60/BRG3.Google Scholar
Witting, L. (2006) A sex-ratio based assessment of common minke whales off West Greenland. Report of the International Whaling Commission SC /58/AWMP3.Google Scholar