Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-31T17:08:15.078Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of polyploidy on response of Dunaliella salina to salinity

Published online by Cambridge University Press:  04 February 2019

Fatemeh Soltani Nezhad  and
Hakimeh Mansouri
Fatemeh Soltani Nezhad
Affiliation:
Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran
Hakimeh Mansouri*
Affiliation:
Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran
*
Author for correspondence: Hakimeh Mansouri, E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

In this study, polyploidy level was determined by flow cytometry analysis. The effect of polyploidy by colchicine treatment was examined on the growth parameters, malondealdehyde (MDA), as well as activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in response to different levels of salinity in Dunaliella salina. The results of algal growth indicated that 3 M NaCl was the optimal concentration of salt, since the highest enhancement in fresh and dry weight, chlorophyll and carotenoids, soluble sugar, glycerol, protein and starch content was observed in comparison to other concentrations. The amount of these metabolites declined in the concentrations under optimum salinity. The least and highest amounts of MDA were observed at 1 and 4 M NaCl respectively. Polyploidy in optimum concentration of salt, caused further increment of the above growth parameters. In relation to this, in most cases, treatment of 0.1% colchicine was most effective. The beneficial effects of polyploidy in non-optimal conditions were also found in some parameters such as biomass, chlorophyll, carotenoids, proteins and starch. Furthermore, the activity of antioxidant enzymes CAT, SOD and POD showed a positive significant correlation with salt stress and these were maximized at 4 M NaCl. Polyploidy (especially colchicine 0.1%) affected activity of these antioxidant enzymes in some concentrations of salt. Overall, our results suggest that the microalgae has significantly different responses to salt stress based on ploidy levels.

Keywords

Antioxidant enzymescarotenoidDunaliella salinaglycerolpolyploidy
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 99 , Issue 5 , August 2019 , pp. 1041 - 1047
Copyright
Copyright © Marine Biological Association of the United Kingdom 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alam, MM, Rezaul Karim, M, Abdul Aziz, M, Monzur Hossain, M, Ahmed, B and Mandal, A (2011) Induction and evaluation of polyploidy in some local potato varieties of Bangladesh. Journal of Biodiversity and Environmental Sciences 1, 16–21.Google Scholar
Avron, M and Ben-Amotz, A (1992) Dunaliella: Physiology, Biochemistry, and Biotechnology. Boca Raton, FL: CRC Press, pp. 125–204.Google Scholar
Azevedo, RA, Alas, RM, Smith, RJ and Lea, PJ (1998) Response of antioxidant enzymes to transfer from elevated carbon dioxide to air and ozone fumigation, in the leaves and roots of wild-type and a catalase-deficient mutant of barley. Physiologia Plantarum 104, 280–292.Google Scholar
Bagheri, M and Mansouri, H (2014) Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L. Applied Biochemistry and Biotechnology 175, 2366–2375.Google Scholar
Ben-Amotz, A (1975) Adaptation of the unicellular alga Dunaliella parva to a saline environment. Journal of Phycology 11, 50–54.Google Scholar
Borowitzka, MA and Siva, CJ (2007) The taxonomy of the genus Dunaliella (Chlorophyta, Dunaliellales) with emphasis on the marine and halophilic species. Journal of Applied Phycology 19, 567–590.Google Scholar
Bradford, MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.Google Scholar
Chitlaru, E and Pick, U (1991) Regulation of glycerol synthesis in response to osmotic changes in Dunaliella. Plant Physiology 96, 50–60.Google Scholar
D'Amato, F and Durante, M (2003) Polyploidy. Online Encyclopaedia of Life Sciences. Chichester: John Wiley & Sons. Available at http://onlinelibrary.wiley.com.Google Scholar
El-Baz, FK, Aboul-Enein, MA, El-Baroty, GS, Youssef, AM and Abd El-Baky, HH (2002) Accumulation of antioxidant vitamins in Dunaliella salina. Online Journal of Biological Science 2, 220–223.Google Scholar
Galbraith, DW, Harkins, KR and Maddox, JM (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220, 1049–1051.Google Scholar
Ghetti, F, Herrmann, H, Häder, DP and Seidlitz, HK (1999) Spectral dependence of the inhibition of photosynthesis under simulated global radiation in the unicellular green alga Dunaliella salina. Journal of Photochemistry and Photobiology. B: Biology 48, 166–173.Google Scholar
Giannopolitis, CN and Ries, SK (1977) Superoxide dismutases. I. Occurrence in higher plants. Plant Physiology 59, 309–314.Google Scholar
Gimmler, H and Molle, REM (1981) Salinity-dependent regulation of starch and glycerol metabolism in Dunaliella parva. Plant, Cell and Environment 4, 367–375.Google Scholar
Gossett, DR, Banks, SW, Millhollon, EP and Luceas, C (1996) Antioxidant response to NaCl stress in a control and NaCl-tolerant cotton cell line grown in the presence of paraquat buthionine sulfoximine, and exogenous glutathione. Plant Physiology 112, 803–809.Google Scholar
Goyal, A (2007) Osmoregulation in Dunaliella, Part I: effects of osmotic stress on photosynthesis, dark respiration and glycerol metabolism in Dunaliella tertiolecta and its salt-sensitive mutant (HL 25/8). Plant Physiology and Biochemistry 45, 696–704.Google Scholar
Grange, S, Leskovar, DI, Pike, LM and Cobb, BG (2003) Seedcoat structure and oxygen-enhanced environments affect germination of triploid watermelon. Journal of American Society for Horticultural Science 128, 253–259.Google Scholar
Hadi, MR, Shariati, M and Afsharzadeh, A (2008) Microalgal biotechnology: carotenoid and glycerol production by the green algae Dunaliella isolated from the Gave-Khooni salt marsh, Iran. Biotechnology and Bioprocess Engineering 13, 540–544.Google Scholar
Heath, RL and Packer, L (1968) Photo peroxidation in isolated chloroplasts. I: kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125, 189–198.Google Scholar
Jahnke, S and White, AL (2003) Long-term hyposaline and hypersaline stresses produce distinct antioxidant responses in the marine alga Dunaliella tertiolecta. Journal of Plant Physiology 160, 1193–1202.Google Scholar
Jaskani, MJ, Kwon, SW and Kim, DH (2005) Comparative study on vegetative, reproductive and qualitative traits of seven diploid and tetraploid watermelon lines. Euphytica 145, 259–268.Google Scholar
Kalita, N, Baruah, G, Goswami, RCD, Talukdar, J and Kalita, MC (2011) Ankistrodesmus falcatus: a promising candidate for lipid production, its biochemical analysis and strategies to enhance lipid productivity. Journal of Microbiology and Biotechnology Research 1, 148–157.Google Scholar
Kirst, G (1990) Salinity tolerance of eukaryotic marine algae. Annual Review of Plant Physiology and Plant Molecular Biology 40, 21–53.Google Scholar
Levine, RL, Garland, D, Olive, C, Amici, A, Clement, I, Lenz, AG, Ahn, BW, Shaltiel, S and Stadtman, ER (1990) Determination of carboxyl content in oxidatively modified proteins. Methods in Enzymology 186, 464–478.Google Scholar
Lichtenthaler, HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in Enzymology 148, 350–382.Google Scholar
Liu, W, Au, DWT, Anderson, DM, Lam, PKS and Wu, RSS (2007) Effects of nutrients, salinity, pH and light: dark cycle on the production of reactive oxygen species in the alga Chattonella marina. Journal of Experimental Marine Biology and Ecology 346, 76–86.Google Scholar
Malanga, G, Calmanovici, G and Puntarulo, S (1997) Oxidative damage to chloroplasts from Chlorella vulgaris exposed to ultraviolet β-radiation. Physiologia Plantarum 101, 455–462.Google Scholar
Massyuk, NP (1973c) Morphology, taxonomy, ecology and geographic distribution of the genus Dunaliella Teod and prospects for its potential utilization. Kiev: Naukova Dumka, pp. 242.Google Scholar
Mensah, JK, Obadoni, BO, Akomeah, PA, Ikhajiagbe, B and Ajibolu, J (2007) The effects of sodium azide and colchicine treatments on morphological and yield traits of sesame seed (Sesame indicum l.). African Journal of Biotechnology 6, 534–538.Google Scholar
Muller, DG (1975) Sex expression in aneuploid gametophytes of the brown alga Ectocarpus siliculosus (Dillw.) Lyngb. Archiv für Protistenkunde 117, 297–302.Google Scholar
Pick, U (2002) Adaptation of the halotolerant alga Dunaliella to high salinity. In Läuchli, A and Lüttge, U (eds), Salinity: Environment, Plants, Molecules. Dordrecht: Kluwer Academic, pp. 97–112.Google Scholar
Raja, R, Iswarya, HS, Balasubramanyam, D and Rengasamy, R (2007) PCR-identification of Dunaliella salina (Volvocales, Chlorophyta) and its growth characteristics. Microbiological Research 162, 168–176.Google Scholar
Rao, AR, Dayananda, C, Sarada, R, Shamala, TR and Ravishankar, GA (2007) Effect of salinity on growth of green alga Botryococcus braunii and its constituents. Bioresource Technology 98, 560–564.Google Scholar
Rijstenbil, JW (2002) Assessment of oxidative stress in the planktonic diatom Thalassiosira pseudonana in response to UV-A and UV-B radiation. Journal of Plankton Research 12, 1277–1288.Google Scholar
Roe, JH (1955) The determination of sugar in blood and spinal fluid with anthrone reagent. Journal of Biological Chemistry 212, 335–343.Google Scholar
Sarma, YSRK (1957) Effects of colchicine on the green alga Sphaeroplea annulina (Roth) Ag. Cytologia 22, 113–117.Google Scholar
Sharma, P, Agarwal, V, Mohan, MK, Kachhwaha, S and Kothari, S (2012) Isolation and characterization of Dunaliella species from Sambhar lake (India) and its phylogenetic position in the genus Dunaliella using 18S rDNA. National Academy Science Letters 35, 207–213.Google Scholar
Sugiyama, S-I (2005) Polyploidy and cellular mechanisms changing leaf size: comparison of diploid and autotetraploid populations in two species of Lolium. Annals of Botany 96, 931–938.Google Scholar
Thayumanavan, B and Sadasivam, S (1984) Physicochemical basis for the preferential uses of certain rice varieties. Plant Foods for Human Nutrition 34, 253–259.Google Scholar
Thomas, DN and Kirst, GO (1991) Salt tolerance of Ectocarpus siliculosus (Dillw.) Lyngb.: comparison of gametophytes, sporophytes and isolates of different geographic origin. Botanica Acta 104, 26–36.Google Scholar
Urbanek, H, Kuzniak-Gebarowska, E and Herka, K (1991) Elicitation of defense responses in bean leaves by Botrytis cinerea polygalacturonase. Acta Physiologiae Plantarum 13, 43–50.Google Scholar
Verslues, PE, Agarwal, M, Katiyar-Agarwal, S, Zhu, J and Zhu, JK (2006) Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant Journal 45, 523–539.Google Scholar
Vonshak, A and Richmond, A (1981) Genome multiplication as related to growth rate in bluegreen algae Anacystic nidulans. Plant and Cell Physiology 22, 1367–1373.Google Scholar
Walne, PL (1966) The effects of colchicine on cellular organization in Chlamydomonas. I. Light microscopy and cytochemistry. American Journal of Botany 53, 908–916.Google Scholar
Wang, Z, Wang, M, Liu, L and Meng, F (2013) Physiological and proteomic responses of diploid and tetraploid black locust (Robinia pseudoacacia L.) subjected to salt stress. International Journal of Molecular Science 14, 20299–20325.Google Scholar
Warner, DA and Edwards, GE (1989) Effects of polyploidy on photosynthetic rates, photosynthetic enzymes, contents of DNA, chlorophyll, and sizes and numbers of photosynthetic cells in the C4 dicot Atriplex confertifolia. Plant Physiology 91, 1143–1151.Google Scholar
Xiong, YC, Li, FM and Zhang, T (2006) Performance of wheat crops with different chromosome ploidy: root-sourced signals, drought tolerance, and yield performance. Planta 224, 710–718.Google Scholar
Xu, L, Najeeb, U, Naeem, MS, Daud, MK, Cao, JS, Gong, HJ, Shen, WQ and Zhou, WJ (2010) Induction of tetraploidy in Juncus effusus by colchicine. Planta 54, 659–663.Google Scholar
Young, AJ and Frank, HA (1996) Energy transfer reactions involving carotenoids: quenching of chlorophyll fluorescence. Journal of Photochemistry and Photobiology B: Biology 36, 3–15.Google Scholar
Zhang, XY, Hu, CG and Yao, JL (2010) Tetraploidization of diploid Dioscorea results in activation of the antioxidant defense system and increased heat tolerance. Journal of Plant Physiology 167, 88–94.Google Scholar