Hostname: page-component-848d4c4894-89wxm Total loading time: 0 Render date: 2024-07-05T10:30:36.892Z Has data issue: false hasContentIssue false

Bactericidal Activity of Coelomic Fluid of the Sea Urchin, Echinus Esculentus, on Different Marine Bacteria

Published online by Cambridge University Press:  11 May 2009

Matthew Service
Affiliation:
University Marine Biological Station, Millport, Isle of Cumbrae, Scotland KA28 oEG
Alastair C. Wardlaw
Affiliation:
Department of Microbiology, University of Glasgow, Glasgow G11 6NU

Extract

Coelomic fluid from the edible sea urchin Echinus esculentus (L.) has in vitro bactericidal activity against the marine pseudomonad, designated strain No. 111 (Wardlaw & Unkles, 1978). This particular bacterial strain was chosen because of its highly characteristic jet-black, agar-digesting colonies on marine agar plates which permitted it to be easily distinguishable from contaminants in bactericidal tests. Bactericidal activity is localised in the cellular components of the coelomic fluid, particularly in the red spherule cells (Messer & Wardlaw, 1980) and is demonstrable in almost all animals tested.

Type
Research Article
Copyright
Copyright © Marine Biological Association of the United Kingdom 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bang, F. B. & Lemma, A., 1962. Bacterial infection and reaction to injury in some echinoderms. Journal of Invertebrate Pathology, 4, 401414.Google Scholar
Johnson, P. T., 1969. The coelomic elements of sea-urchins (Strongylocentrotus). III. In vitro reaction to bacteria. Journal of Invertebrate Pathology, 13, 4262.CrossRefGoogle Scholar
Johnson, P. T. & Chapman, F. A., 1970. Comparative studies on the in vitro response of bacteria to invertebrate body fluids. I. Dendrostomum zostericolum, a sipunculid worm. Journal of Invertebrate Pathology, 16, 127138.CrossRefGoogle ScholarPubMed
Johnson, P. T. & Chapman, F. A., 1971. Comparative studies on the in vitro response of bacteria to invertebrate body fluids. III. Stichopus tremulus (sea cucumber) and Dendraster excentricus (sand dollar). Journal of Invertebrate Pathology, 16, 127138.CrossRefGoogle Scholar
Jollès, J. & Jollès, J., 1975. The lysozyme from Asterias rubens. European Journal of Biochemistry, 54, 1923.CrossRefGoogle ScholarPubMed
Messer, L. I. & Wardlaw, A. C., 1980. Separation of the coelomocytes of Echinus esculentus by density gradient centrifugation. In Echinoderms: Present and Past. Proceedings of the European Colloquium on Echinoderms, Brussels, 1979 (ed. Jangoux, M.), pp. 319323. Rotterdam: A. A. Balkema.Google Scholar
Perkins, F. T., Sheffield, F. W., Outschoorn, A. S. & Hemsley, D. A., 1973. An international collaborative study on the measurement of the opacity of bacterial suspensions. Journal of Biological Standardization, 1, 110.CrossRefGoogle Scholar
Ryoyama, K., 1973. Studies on the biological properties of coelomic fluid of sea urchin. I. Naturally occurring hemolysin in sea urchin. Biochimica et biophysica acta, 320, 157165.CrossRefGoogle ScholarPubMed
Ryoyama, K., 1974. Studies on the biological properties of coelomic fluid of sea urchin. II. Naturally occurring hemagglutinin in sea urchin. Biological Bulletin. Marine Biological Laboratory, Woods Hole, Mass., 146, 404414.CrossRefGoogle ScholarPubMed
Service, M. & Wardlaw, A. C., 1984. Echinochrome-A as a bactericidal substance in the coelomic fluid of Echinus esculentus (L.). Comparative Biochemistry and Physiology, 79B, 161—165.Google Scholar
Shewan, J. M., Hobbs, G. & Hodgkiss, W., 1960. A determinative scheme for the identification of certain genera of gram-negative bacteria, with special reference to the Pseudomonadaceae. Journal of Applied Bacteriology, 23, 379390.CrossRefGoogle Scholar
Wardlaw, A. C. & Unkles, S. E., 1978. Bactericidal activity of coelomic fluid from the sea urchin Echinus esculentus. Journal of Invertebrate Pathology, 32, 2534.CrossRefGoogle Scholar