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Aplysina (Porifera: Demospongiae) species identification through SSCP-ITS patterns

Published online by Cambridge University Press:  24 November 2009

Flávia Rachel M. Lamarão ,
Estéfane C. Reis ,
Tatiana A. Simão ,
Rodolpho M. Albano  and
Gisele Lôbo-Hajdu
Flávia Rachel M. Lamarão
Affiliation:
Departamento de Genética, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524—PHLC—Sala 205, 20550-013, Rio de Janeiro, RJ, Brazil Departamento de Hanseníase, IOC, Fiocruz, Avenida Brasil, 4365—Manguinhos, 21040-360, Rio de Janeiro, RJ, Brazil Departamento de Bioquímica, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87 (fundos, 4° andar), 20551-013, Rio de Janeiro, RJ, Brazil
Estéfane C. Reis
Affiliation:
Departamento de Genética, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524—PHLC—Sala 205, 20550-013, Rio de Janeiro, RJ, Brazil
Tatiana A. Simão
Affiliation:
Departamento de Bioquímica, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87 (fundos, 4° andar), 20551-013, Rio de Janeiro, RJ, Brazil
Rodolpho M. Albano
Affiliation:
Departamento de Bioquímica, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87 (fundos, 4° andar), 20551-013, Rio de Janeiro, RJ, Brazil
Gisele Lôbo-Hajdu*
Affiliation:
Departamento de Genética, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524—PHLC—Sala 205, 20550-013, Rio de Janeiro, RJ, Brazil
*
Correspondence should be addressed to: G. Lôbo-Hajdu, Departamento de Genética, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524—PHLC—Sala 205, 20550-013, Rio de Janeiro, RJ, Brazil email: [email protected]
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Abstract

Keratose sponges have no spicules and limited robust morphological characters, causing severe problems for taxonomic classification. Single-strand conformational polymorphism (SSCP) analysis of PCR amplified internal transcribed spacers (ITS) of nuclear ribosomal RNA (rRNA) genes was used to elucidate speciation patterns in marine sponges of the genus Aplysina. In this study, Aplysina species with non-ambiguous traditional morphological alpha taxonomy were used to generate standard SSCP-ITS patterns, which were compared with patterns from alleged new species. Our results show that ITS-SSCP patterns allowed the differentiation of Aplysina sponges, demonstrating the advantage of a method that explores the secondary structure compared to direct sequencing.

Keywords

marine spongesinternal transcribed spacersPCR-SSCPcryptic species
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 90 , Issue 4 , June 2010 , pp. 845 - 850
Copyright
Copyright © Marine Biological Association of the United Kingdom 2009

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