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An assessment of the basis of mercury tolerance in Dunaliella tertiolecta

Published online by Cambridge University Press:  11 May 2009

Anthony G. Davies
Anthony G. Davies
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth
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Abstract

The specific growth rate of Dunaliella tertiolecta was unaffected by mercury II concentrations of at least 2.03 μg at/1. At 10 μg at/1, it was eventually reduced by 84% but growth continued, giving a final level of cell material only 13% below that in a mercuryfree control. At this concentration, however, growth was largely uncoupled from division and giant cells were produced, probably due to the effect of mercury upon the production of methionine which is known to be implicated in the process of cell division.

The basis of the mercury tolerance was investigated in terms of (1) mercury detoxication in the culture medium by complex or compound formation between the metal and metabolites produced by the cells, (2) the concentration of sulphydryl groups both within the cells as possible sequestration sites and in the cell membrane where any molecular disruption and permeability changes produced by the metal first occur, (3) the absence of cellular potassium leakage and (4) the resistance of the cell membrane to the uptake of mercury II ions. Where possible, the results were compared with those from determinations of the same properties of the mercury-sensitive species Isochrysis galbana. The experiments indicated that the mercury tolerance of D. tertiolecta is partly related to the slower rate of mercury accumulation by this species, but is largely due to the detoxication of the mercury within the cell possibly by the precipitation of a highly insoluble mercury compound

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 56 , Issue 1 , February 1976 , pp. 39 - 57
Copyright
Copyright © Marine Biological Association of the United Kingdom 1976

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References

Antonovics, J., Bradshaw, A. D. & Turner, R. G., 1971. Heavy metal tolerance in plants. Advances in Ecological Research, 7, 1–85.Google Scholar
Ashida, J., 1965. Adaptation of fungi to metal toxicants. Annual Review of Phytopathology, 3, 153–74.Google Scholar
Ashida, J., Higashi, N. & Kikuchi, T., 1963. An electronmicroscopic study on copper precipitation by copper resistant yeast cells. Protoplasma, 57, 27–32.CrossRefGoogle Scholar
Barber, R. T. & Ryther, J. H., 1969. Organic chelators: factors affecting production in the Cromwell Current upwelling. Journal of Experimental Marine Biology and Ecology, 3, 191–9.CrossRefGoogle Scholar
Chopra, I., 1971. Decreased uptake of cadmium by a resistant strain of Staphylococcus aureus. Journal of General Microbiology, 63, 265–7.Google Scholar
Craigie, J. S. & McLachlan, J., 1964. Glycerol as a photosynthetic product in Dunaliella tertiolecta Butcher. Canadian Journal of Botany, 42, 777–8.CrossRefGoogle Scholar
Craigie, J. S., McLachlan, J., Majak, W., Ackman, R. G. & Tocher, C. S., 1966. Photosynthesis in algae. II. Green algae with special reference to Dunaliella spp. and Tetraselmis spp. Canadian Journal of Botany, 44, 1247–54.CrossRefGoogle Scholar
Crank, J., 1970. The mathematics of diffusion, vi, 347 pp. Oxford University Press.Google Scholar
Davey, E. W., Morgan, M. J. & Erickson, S. J., 1973. A biological measurement of the copper complexation capacity of sea water. Limnology and Oceanography, 18, 993–7.CrossRefGoogle Scholar
Davies, A. G., 1973. The kinetics of and a preliminary model for the uptake of radio-zinc by Phaeodactylum tricornutum in culture. In Radioactive contamination of the marine environment. Proceedings of a symposium, Seattle, 1972, 403–20. Vienna: International Atomic Energy Agency.Google Scholar
Davies, A. G., 1974. The growth kinetics of Isochrysis galbana in cultures containing sublethal concentrations of mercuric chloride. Journal of the Marine Biological Association of the United Kingdom, 54, 157–69.CrossRefGoogle Scholar
Guillard, R. R. L. & Wangersky, P. J., 1958. The production of extracellular carbohydrates by some marine flagellates. Limnology and Oceanography, 3, 449–54.CrossRefGoogle Scholar
Helfferich, F., 1962. Ion exchange, ix, 624 pp. London: McGraw-Hill Book Company.Google Scholar
Hellebust, J. A., 1965. Excretion of some organic compounds by marine phytoplankton. Limnology and Oceanography, 10, 192–206.Google Scholar
Heyrovsky, J. & Kuta, J., 1966. Principles of polarography. 581 pp. London: Academic Press.Google Scholar
Huntsman, S. A., 1972. Organic excretion by Dunaliella tertiolecta. Journal of Phycology, 8, 59–63.Google Scholar
Huntsman, S. A. & Barber, R. T., 1975. Modification of phytoplankton growth by excreted compounds in low-density populations. Journal of Phycology, 11, 10–13.Google Scholar
Johnston, R., 1964. Sea water, the natural medium of phytoplankton. II. Trace metals and chelation, and general discussion. Journal of the Marine Biological Association of the United Kingdom, 44, 87–110.Google Scholar
Jonsson, A., Quarfort, I. & Sillén, L. G., 1947. Electrometric investigations of equilibrium between mercury and halogen ions. III. The ‘millimolar’ potentials of mercury and the solubility product of mercury I chloride. Acta chemica scandinavica, 1, 479–88.CrossRefGoogle Scholar
Mandelli, E. F., 1969. The inhibitory effects of copper on marine phytoplankton. Contributions in Marine Science, University of Texas, 14, 47–57.Google Scholar
Nelson, N., 1944. A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry, 153, 375–80.CrossRefGoogle Scholar
Olafson, R. W. & Thompson, J. A. J., 1974. Isolation of heavy metal binding proteins from marine vertebrates. Marine Biology, 28, 83–6.CrossRefGoogle Scholar
Overnell, J., 1975. The effect of heavy metals on photosynthesis and loss of cell potassium in two species of marine algae, Dunaliella tertiolecta and Phaeodactylum tricornutum. Marine Biology, 29, 99–103.CrossRefGoogle Scholar
Passow, H., 1970. The red blood cell: penetration, distribution and toxic action of heavy metals. In Effects of metals on cells, subcellular elements and macromolecules, eds J., Maniloff, Coleman, J. R. and Miller, M. W., 291–340. Springfield, Illinois: Charles C. Thomas.Google Scholar
Passow, H. & Rothstein, A., 1960. The binding of mercury by the yeast cell in relation to changes in permeability. Journal of General Physiology, 43, 621–33.Google Scholar
Radoslovich, E. W., Raupach, M., Slade, P. G. & Taylor, R. M., 1970. Crystalline cobalt, zinc, manganese and iron alkoxides of glycerol. Australian Journal of Chemistry, 23, 1963–71.CrossRefGoogle Scholar
Richards, F. A., 1965. Anoxic basins and fjords. In Chemical oceanography, eds Riley, J. P. & Skirrow, G., Vol. 1, 611–45. London: Academic Press.Google Scholar
Rothstein, A., 1959. Cell membrane as site of action of heavy metals. Federation Proceedings. Federation of American Societies for Experimental Biology, 18, 1026–38.Google Scholar
Schiff, J. A., 1959. Studies on sulfate utilization by Chlorella pyrenoidosa using sulfate-35S; the occurrence of S-adenosyl methionine. Plant Physiology, 34, 73–80.CrossRefGoogle Scholar
Shrift, A., 1959. Nitrogen and sulfur changes associated with growth uncoupled from cell division in Chlorella vulgaris. Plant Physiology, 34, 505–12.CrossRefGoogle ScholarPubMed
Schwarzenbach, G. & Widmer, M. 1963. Die Löslichkeit von metallsulfiden I. Schwarzes quecksilbersulfid. Helvetica chimica acta, 46, 2613–28.Google Scholar
Shaw, W. H. R., 1954. Toxicity of cations toward living systems. Science, New York, 120, 361–3.CrossRefGoogle Scholar
Shieh, Y. J. & Barber, J., 1973. Uptake of mercury by Chlorella and its effect on potassium regulation. Planta, 109, 49–60.CrossRefGoogle ScholarPubMed
Steemann Nielsen, E. & Wium-Anderson, S. 1970. Copper ions as poison in the sea and in freshwater. Marine Biology, 6, 93–7.Google Scholar
Strickland, J. D. H. & Parsons, T. R., 1972. A practical handbook of seawater analysis, 2nd ed. Bulletin of the Fisheries Research Board of Canada, No. 167, 310 pp.Google Scholar
Vaczi, L., Fodor, M., Milch, H. & Rethy, A., 1962. Studies on the mercuric chloride resistance of Staphylococcus aureus. Acta microbiologica, 9, 81–7.Google Scholar
VanSteveninck, J., Weed, R. I. & Rothstein, A., 1965. Localization of erythrocyte membrane sulfhydryl groups essential for glucose transport. Journal of General Physiology, 48, 617–32.Google Scholar
Weed, R., Eber, J. & Rothstein, A., 1962. Interaction of mercury with human erythrocytes. Journal of General Physiology, 45, 395–410.CrossRefGoogle ScholarPubMed