Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-22T17:45:05.709Z Has data issue: false hasContentIssue false
  • English
  • Français

Addendum to A Micro-Analytical Scheme for the Biochemical Analysis of Marine Invertebrate Larvae

Published online by Cambridge University Press:  11 May 2009

D. L. Holland  and
P. J. Hannant
D. L. Holland
Affiliation:
NERC Unit of Marine Invertebrate Biology, Marine Science Laboratories, Menai Bridge, Anglesey, N. Wales, U.K.
P. J. Hannant
Affiliation:
NERC Unit of Marine Invertebrate Biology, Marine Science Laboratories, Menai Bridge, Anglesey, N. Wales, U.K.
Get access Rights & Permissions [Opens in a new window]

Extract

A micro-analytical scheme for the determination of protein, carbohydrate, lipid and RNA levels in marine invertebrate larvae has been described in a previous paper (Holland & Gabbott, 1971). This addendum describes minor modifications to the scheme so that the level of DNA may be determined and includes a different procedure for the determination of RNA by u.v. absorption. DNA and RNA levels were determined in a number of nauplii and veliger larvae.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 53 , Issue 4 , November 1973 , pp. 833 - 838
Copyright
Copyright © Marine Biological Association of the United Kingdom 1973

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Brachet, J., 1960. The biochemistry of development. In International Series of Monographs on Pure and Applied Biology. Division: Modern trends in Physiological Sciences (ed. P., Alexander and Bacq, Z. M.), 320 pp. Oxford, London, New York, Paris: Pergamon Press.Google Scholar
Bulow, F. J., 1970. RNA-DNA ratios as indicators of recent growth rates of a fish. Journal of the Fisheries Research Board of Canada, 27, 2343–49.CrossRefGoogle Scholar
Ceriotti, G., 1952. A microchemical determination of desoxyribonucleic acid. Journal of Bio-logical Chemistry, 198, 297–303.CrossRefGoogle ScholarPubMed
Corner, E. D. S. & Cowey, C. B., 1968. Biochemical studies on the production of marine zoo-plankton. Biological Reviews, 43, 393–426.CrossRefGoogle Scholar
Dagg, M. J. & Littlepage, J. C., 1972. Relationships between growth rate and RNA, DNA protein and dry weight in Anemia salina and Euchaeta elongata. Marine Biology, 17, 162–70.CrossRefGoogle Scholar
Elson, D., Gustafson, T. & Chargaff, E., 1954. The nucleic acids of the sea-urchin during embryonic development. Journal of Biological Chemistry, 209, 285–94.CrossRefGoogle ScholarPubMed
Gruffydd, Ll. D. & Beaumont, A. R., 1970. Determination of the optimum concentration of eggs and spermatozoa for the production of normal larvae in Pecten maximus (Mollusca, Lamellibranchia). Helgoländer wissenschaftliche Meeresuntersuchungen, 20, 486–97.CrossRefGoogle Scholar
Haines, T. A., 1973. An evaluation of RNA-DNA ratio as a measure of long-term growth in fish populations. Journal of the Fisheries Research Board of Canada, 30, 195–99.CrossRefGoogle Scholar
Helm, M. M., Holland, D. L. & Stevenson, R. R., 1973. The effect of supplementary algal feeding of a hatchery breeding stock of Ostrea edulis L. on larval vigour. Journal of the Marine Biological Association of the United Kingdom, 53, 673–84.CrossRefGoogle Scholar
Holland, D. L. & Gabbott, P. A., 1971. A micro-analytical scheme for the determination of protein, carbohydrate, lipid and RNA levels in marine invertebrate larvae. Journal of the Marine Biological Association of the United Kingdom, 51, 659–68.CrossRefGoogle Scholar
Holland, D. L. & Spencer, B. E., 1973. Biochemical changes in fed and starved oysters, Ostrea edulis L., during larval development, metamorphosis and early spat growth. Journal of the Marine Biological Association of the United Kingdom, 53, 287–98.CrossRefGoogle Scholar
Keck, K., 1956. An ultramicro technique for the determination of deoxypentose nucleic acid. Archives of Biochemistry and Biophysics, 63, 446–51.CrossRefGoogle ScholarPubMed
Lowry, O. M., Rosebrough, N. J., Farr, A. L. & Randall, R. J., 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265–75.CrossRefGoogle ScholarPubMed
Marsh, J. B. & Weinstein, D. B., 1966. Simple charring method for determination of lipids. Journal of Lipid Research, 1, 574–6.CrossRefGoogle Scholar
Munro, H. N. & Fleck, A., 1966. The determination of nucleic acids. In Methods of Biochemical Analysis (ed. D., Glick), 14, 113–76. New York and London: Interscience.CrossRefGoogle Scholar
Sutcliffe, W. H., 1965. Growth estimates from ribonucleic acid content in some small animals. Limnology and Oceanography, 10 (Suppl.), R. 253–8.CrossRefGoogle Scholar
Sutcliffe, W. H., 1970. Relationship between growth rate and ribonucleic acid concentration in some invertebrates. Journal Fisheries Research Board of Canada, 27, 606–9.CrossRefGoogle Scholar
Tighe-Ford, D. J., Power, M. J. D. & Vaile, D. C., 1970. Laboratory rearing of barnacle larvae for antifouling research. Helgoldnder wissenschaftliche Meeresuntersuchungen, 20, 393–405.CrossRefGoogle Scholar