Since the Report on the subject of the Artificial Culture of Sponges was published, some further information of importance relating to the subject has been courteously supplied by the Acting Commissioner of the United States Commission of Fish and Fisheries. This information is in the form of a letter to the United States Commission from Mr. Ralph M. Monroe, of Cocoanut Grove, Biscayne Bay, Florida, to whom the Acting Commissioner refers as “an intelligent and energetic man, whose statements, we think, can be given entire credence” wherein this gentleman gives a detailed account of some experiments conducted by himself at Biscayne Bay, during the years 1889, 1890 and 1891.