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A pelagic marine diatom requiring cobalamin

Published online by Cambridge University Press:  11 May 2009

M. R. Droop
Affiliation:
The Marine Station, Millport

Extract

A bacteria-free culture of the important centric diatom Skeletonema costatum has recently been established at Millport, and some exploratory nutritional experiments have been carried out with it. S. costatum is a photo-autotroph whose autotrophy is, apparently, limited to cobalamin (vitamin B12).

Details concerning isolation and maintenance of S. costatum differ from those already published (Droop, 1954a, 1955) only in the matter of pH control, a vital factor in the successful culture of this species whose range of tolerance is narrow (pH 7–5–8–5).

Type
Research Article
Copyright
Copyright © Marine Biological Association of the United Kingdom 1955

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