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Distribution pattern of GFP (green fluorescent protein) in a bivalve-inhabiting hydrozoan, Eutima japonica (Leptomedusae: Eirenidae)

Published online by Cambridge University Press:  14 July 2010

Shin Kubota ,
Eriko Nomaru ,
Hiroko Uchida  and
Akio Murakami
Shin Kubota*
Affiliation:
Seto Marine Biological Laboratory, Field Science Education and Research Center, Kyoto University, Shirahama, Nishimuro, Wakayama 649-2211, Japan
Eriko Nomaru
Affiliation:
Kobe University Research Center for Inland Seas, Iwaya 2746, Awaji, Hyogo, 656-401, Japan
Hiroko Uchida
Affiliation:
Kobe University Research Center for Inland Seas, Iwaya 2746, Awaji, Hyogo, 656-401, Japan
Akio Murakami
Affiliation:
Kobe University Research Center for Inland Seas, Iwaya 2746, Awaji, Hyogo, 656-401, Japan
*
Correspondence should be addressed to: S. Kubota, Seto Marine Biological Laboratory, Field Science Education and Research Center, Kyoto University, Shirahama, Nishimuro Wakayama 649-2211Japan email: [email protected]
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Abstract

Bright green auto-fluorescence was observed in the umbrellar margin, umbrellar marginal warts, tentacular bulbs, tentacles, and manubrium of laboratory-reared immature (1–14 days old) medusae of Eutima japonica from Japan and China. In vivo microscopic fluorescence spectra showed that the green fluorescence was similar to that of green fluorescent protein (GFP) found in Aequorea victoria, although the maximum emission wavelength (503 nm) was slightly bluer. No fluorescence was detected in the cirri, statocysts, radial canals, velum, or subumbrella of the medusae. The fluorescence distribution pattern in E. japonica more closely resembles that of Eugymnanthea inquilina from the Mediterranean Sea than that of Japanese Eugymnanthea japonica, which is the derived species of E. japonica. This suggests that the common fluorescence pattern is convergently evolved in the former two species, perhaps owing to the as yet unclarified physiological and/or ecological function of GFP and/or GFP-like proteins.

Keywords

fluorescence pattern and spectragreen fluorescent protein (GFP)hydromedusaEutimaEugymnantheasystematics
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 90 , Issue 7 , November 2010 , pp. 1371 - 1374
Copyright
Copyright © Marine Biological Association of the United Kingdom 2010

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