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Detection of High Levels of Genetic Relatedness in Rock-Populations of an Intertidal Isopod Using DNA Fingerprinting

Published online by Cambridge University Press:  11 May 2009

Stuart B. Piertney  and
Gary R. Carvalho
Stuart B. Piertney
Affiliation:
Present address: Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB9 2TN
Gary R. Carvalho
Affiliation:
Marine & Fisheries Genetics Laboratory, School of Biological Sciences, University College of Swansea, Singleton Park, Swansea, SA2 8PP.
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Extract

Multi-locus DNA fingerprinting was used to determine the degree of genetic relatedness within localized assemblages of the intertidal isopod, Jaera albifrons (Crustacea: Isopoda). This isopod has marsupial development, restricted adult vagility and specific habitat preferences, which will tend to isolate groups of animals on a shore and can potentially maintain the integrity of broods after release from the mother. Total genomic DNA was extracted from six individuals from each of ten ‘rock-populations’ in the South Wales area, restricted with Rsal, Alulll and Haelll endonucleases, and probed using a (TCC)5 oligonucleotide probe. Band-sharing analysis of the fingerprint patterns indicated significantly higher genetic relatedness within rock-populations (mean within rock-population band-sharing value = 0–374 ±0–023 SE) than between (maximum between rock-population band-sharing value = 0–301 ±0033), and at a level that equates to a relatedness between individuals within rock-populations of slightly below half-sibling status. The significance of such findings, in terms of the potential for localized inbreeding and population genetic structure, is discussed.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 75 , Issue 4 , November 1995 , pp. 967 - 976
Copyright
Copyright © Marine Biological Association of the United Kingdom 1995

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