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Biochemical composition and function of jelly masses of Marphysa gravelyi (Polychaeta: Eunicidae) from Pulicat Lake, India

Published online by Cambridge University Press:  23 March 2017

E. Malathi ,
Collette M Juliana  and
Priscilla A Lyndsay
E. Malathi*
Affiliation:
Department of Zoology, Queen Mary's College, Chennai, India
Collette M Juliana
Affiliation:
Department of Zoology, Queen Mary's College, Chennai, India
Priscilla A Lyndsay
Affiliation:
Department of Zoology, Queen Mary's College, Chennai, India
*
Correspondence should be addressed to: E. Malathi, Department of Zoology, Queen Mary's College, Chennai, India email: [email protected]
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Abstract

Eggs of Marphysa gravelyi are spawned in gelatinous masses and the developing larvae are harboured in them until they are ready to undergo settlement. In order to understand the function of the jelly mass, morphometric, histochemical, biochemical and antimicrobial analyses were performed. The observations indicate the fibrous jelly mass is composed of carbohydrate, protein and lipid, and size is correlated to number of eggs present within. Extracts from the jelly mass of Marphysa gravelyi exhibit inhibitory activity against Escherichia coli, Vibrio vulnificus and Candida albicans but no activity was seen against seven other microorganisms tested. The results show that the function of the jelly mass is to nourish the developing embryos, protect against desiccation and predation from macrofauna, and most importantly prevent the dispersal of the juveniles from the desirable habitat.

Keywords

Marphysa gravelyiPolychaetajelly massmorphometricsbiochemical compositionantimicrobial properties
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 97 , Special Issue 5: Proceedings of the 12th International Polychaete Conference Amgueddfa Cymru - National Museum Wales Cardiff, Wales, UK, 1-5 August 2016 , August 2017 , pp. 1135 - 1141
Copyright
Copyright © Marine Biological Association of the United Kingdom 2017 

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References

REFERENCES

Aiyar, R.G. (1931) An account of the development and breeding-habits of a brackish water polychaete worm of the genus Marphysa . Zoological Journal of the Linnean Society 37, 387–403.Google Scholar
Barnes, H. and Blackstock, J. (1973) Estimation of lipids in marine animals and tissues: detailed investigation of the sulphophosphovanilun method for total lipids. Journal of Experimental Marine Biology and Ecology 12, 103–118.CrossRefGoogle Scholar
Benkendorff, K., Davis, A.R. and Bremner, J. (2001) Chemical defense in the egg masses of benthic invertebrates: an assessment of antibacterial activity in 33 mollusks and 4 polychaetes. Journal of Invertebrate Pathology 78, 109–118.Google Scholar
Biggs, H.G. and Moorehead, W.R. (1974) O-Cresolpthalein direct colorimetric method. Clinical Chemistry 20, 1458–1460.Google Scholar
Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.Google Scholar
Brommer, J.E. (2000) The evolution of fitness in life history theory. Biological Reviews 75, 377–404.Google Scholar
Chapman, G. (1965) The egg cocoons of Scoloplos armiger O.F. Miller. The Biological Bulletin. Department of Woods Hole, Massachusetts, no 128, 8 pp.CrossRefGoogle Scholar
Chiffelle, T.L. and Putt, F.A. (1951) Propylene and ethylene glycol as solvents for Sudan IV and Sudan black B. Stain Technology 26, 51–56.Google Scholar
Cuvillier-Hot, V., Boidin-Wichlacz, C. and Tasiemski, A. (2014) Polychaetes as annelid models to study ecoimmunology of marine organisms. Journal of Marine Science and Technology 22, 9–14.Google Scholar
Folch, J., Lees, M. and Sloane Stanley, G.H. (1957) A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Giangrande, A. (1997) Polychaete reproductive patterns, life cycles and life histories: an overview. Oceanography and Marine Biology – An Annual Review 35, 323–386.Google Scholar
Gurr, E. (1956) A practical manual of medical and biological staining techniques. New York, NY: Interscience Publishers.Google Scholar
Harding, S., Davis, S., Deacon, M. and Fiebrig, I. (1999) Biopolymer mucoadhesives. Biotechnology and Genetic Engineering Reviews 16, 41–86.Google Scholar
Humason, G.L. (1979) Animal tissue techniques. San Francisco: W.H. Freeman.Google Scholar
Izzo, C., Grub, F. and Murador, E. (1981) Improved method for determination of high-density-lipoprotein cholesterol I. Isolation of high-density lipoproteins by use of polyethylene glycol 6000. Clinical Chemistry 27, 371–374.Google Scholar
Lillie, R.D. and Fullmer, H.M. (1976) Histopathologic technique and practical histochemistry. New York, NY: McGraw-Hill.Google Scholar
Malathi, E., Sunder Raj, S.K. and Mercy Bai, P. (2011) Larval development of Marphysa gravelyi (Polychaeta: Eunicidae) from Pulicat Lake, India. Italian Journal of Zoology 78(Suppl. 1), 249–254.Google Scholar
Mowry, R.W. (1956) Alcian blue technique for histochemical study of acidic carbohydrates. Journal of Histochemistry and Cytochemistry 4, 407.Google Scholar
Nakamura, M., Taniguti, Y., Yamamoto, M., Hino, K. and Manabe, M. (1997) Homogenous assay of serum LDL cholesterol on an autoanalyzer. Clinical Chemistry 43, 260–261.Google Scholar
Pechenik, J.A. (1978) Adaptations to intertidal development: studies on Nassarius obsoletus . Biological Bulletin 154, 282–291.Google Scholar
Pechenik, J.A. (1983) Egg capsules of Nucella lapillus (L.) protect against low-salinity stress. Journal of Experimental Marine Biology and Ecology 71, 165–179.Google Scholar
Racker, E. (1946) Spectrophotometric measurement of hexokinase and phosphohexokinase activity. Journal of Biological Chemistry 167, 843–854.CrossRefGoogle Scholar
Roe, J.H. and Dailey, R.E. (1966) Determination of glycogen with the anthrone reagent. Analytical Biochemistry 15, 245–250.Google Scholar
Schwarzenbach, G. (1955) The complexones and their analytical application. Analyst 80, 713–729.Google Scholar
Scott, J.E. and Dorling, J. (1965) Differential staining of acid glycosaminoglycans (mucopolysaccharides) by alcian blue in salt solutions. Histochemie 5, 221.Google Scholar
Southern, R. (1921) Polychaeta of the Chilka Lake and also of fresh and brackish waters in other parts of India. Memoirs of the Indian Museum 5, 563–659.Google Scholar
Southgate, H.W. (1927) Note on preparing mucicarmine. Journal of Pathology and Bacteriology 30, 729–730.Google Scholar
Sugiura, A., Oikawa, T., Hirano, K., Maeda, H., Yoshimura, H., Sugiyama, M. and Kuratsu, T. (1977) A simple colorimetric method for determination of serum triglycerides with lipoprotein lipase and glycerol dehydrogenase. Clinica Chimica Acta 81, 125–130.Google Scholar
Thorson, G. (1946) Reproduction and larval development of Danish marine bottom invertebrates with special reference to the planktonic larvae in the Sound. Meddelelser fra Kommissionen for Danmarks fiskeri og havundersøgelser. Serie Plankton, bd. 4, 1–523.Google Scholar
Wilson, W.H. Jr (1991) Sexual reproductive modes in polychaetes: classification and diversity. Bulletin of Marine Science 48, 500–516.Google Scholar
Woodcock, S.H. and Benkendorff, K. (2008) The impact of diet on the growth and proximate composition of juvenile whelks, Dicathaisorbita (Gastropoda: Mollusca). Aquaculture 276, 162–170.Google Scholar