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Salivary clusterin as a biomarker of tobacco consumption in nicotine addicts undergoing smoking cessation therapy

Published online by Cambridge University Press:  14 April 2020

Iñigo Pallardo-Fernández ,
Victoria Iglesias ,
Carmen Rodríguez-Rivera ,
Carmen González-Martín  and
Luis F. Alguacil Open the ORCID record for Luis F. Alguacil [Opens in a new window]
Iñigo Pallardo-Fernández
Affiliation:
Translational Addiction Research Group, San Pablo CEU University, Alcorcón, Spain
Victoria Iglesias
Affiliation:
Translational Addiction Research Group, San Pablo CEU University, Alcorcón, Spain CMSc Fuencarral, Madrid Salud, Madrid, Spain
Carmen Rodríguez-Rivera
Affiliation:
Translational Addiction Research Group, San Pablo CEU University, Alcorcón, Spain
Carmen González-Martín
Affiliation:
Translational Addiction Research Group, San Pablo CEU University, Alcorcón, Spain
Luis F. Alguacil*
Affiliation:
Translational Addiction Research Group, San Pablo CEU University, Alcorcón, Spain
*
Author for correspondence: Luis F. Alguacil, E-mail: [email protected]
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Abstract

Introduction

Recent studies have led to suggest that the multifunctional protein clusterin could be envisaged as a potential diagnostic biomarker of addictive behaviours. However, this hypothesis has not been yet tested in nicotine addicts.

Aims

We have studied possible associations between clusterin levels in saliva from smokers under cessation treatment and variables related to tobacco consumption, dependence and addiction.

Methods

Eighty-one patients were included in an ambulatory smoking cessation programme that involved the use of pharmacological and behavioural therapy. The participants underwent psychological assessment of addiction and dependence (DAST-20, ASSIST, Fagerström tests) and provided saliva samples at the onset of the intervention and 6 months after smoking discontinuation to study the evolution of clusterin levels by enzyme-linked immunosorbent assays.

Results

Clusterin concentration did not correlate with nicotine addiction/dependence scores but was significantly elevated in smokers with prolonged tobacco use and high intensity of tobacco consumption. Moreover, the levels of the protein significantly decreased 6 months after smoking cessation.

Conclusions

The results obtained provide strong evidence of a close association between tobacco use and salivary clusterin, a protein that emerges as a biomarker of tobacco toxicity with potential interest to monitor the beneficial effects of smoking cessation.

Keywords

Clusterinsmoking cessation
Type
Brief Report
Information
Journal of Smoking Cessation , Volume 15 , Issue 3 , September 2020 , pp. 171 - 174
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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