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Development of radioresistance in drug resistant human MCF-7 breast cancer cells

Published online by Cambridge University Press:  01 December 2009

Meltem Demirel Kars ,
Özlem Darcansoy Iseri ,
Ali Ugur Ural ,
Ferit Avcu ,
Murat Beyzadeoglu ,
Bahar Dirican  and
Ufuk Gündüz
Meltem Demirel Kars
Affiliation:
Department of Biological Sciences, Middle East Technical University, Ankara, Turkey
Özlem Darcansoy Iseri
Affiliation:
Department of Biological Sciences, Middle East Technical University, Ankara, Turkey
Ali Ugur Ural
Affiliation:
Department of Hematology, School of Medicine, Gülhane Military Academy, Ankara, Turkey
Ferit Avcu
Affiliation:
Department of Hematology, School of Medicine, Gülhane Military Academy, Ankara, Turkey
Murat Beyzadeoglu
Affiliation:
Department of Radiation Oncology, School of Medicine, Gülhane Military Academy, Ankara, Turkey
Bahar Dirican
Affiliation:
Department of Radiation Oncology, School of Medicine, Gülhane Military Academy, Ankara, Turkey
Ufuk Gündüz
Affiliation:
Department of Radiation Oncology, School of Medicine, Gülhane Military Academy, Ankara, Turkey
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Abstract

Background and purpose: Radiotherapy is used for the treatment of malignant tumours, and may be used as the primary therapy. It is also common to combine radiotherapy with surgery, chemotherapy, hormone therapy or some combination of them. Even if the tumour is treated intensively, women diagnosed with breast cancer may develop a recurrence. Most recurrences may be in the form of distant metastases, development of multi-drug resistance phenotype or both together. This study demonstrated that some of the multi-drug resistant cancer cells may also become radioresistant.

Materials and Methods: Chemoresistance in paclitaxel (MCF-7/Pac), docetaxel (MCF-7/Doc), vincristine (MCF-7/Vinc), doxorubicin (MCF-7/Dox) and zoledronic acid (MCF-7/Zol) resistant MCF-7 cells were demonstrated by XTT assay. MDR1 gene expression was detected by real-time PCR in human MCF-7 breast cancer cells. Drug resistant and sensitive cells were exposed to γ-radiation and development of radioresistance was investigated.

Results: Results have indicated that paclitaxel, docetaxel, vincristine, doxorubicin and zoledronic acid–selected cells gained varying degrees of resistance to their selective drugs when compared with original MCF-7/S. MCF-7/Pac, MCF-7/Doc, MCF-7/Vinc and MCF-7/Dox cells have all acquired MDR1 expression. Among the resistant sub-lines, MCF-7/Pac and MCF-7/Doc cells were significantly cross-resistant to irradiation compared to the sensitive cells.

Conclusion: MCF-7/Pac and MCF-7/Doc cell lines were found radioresistant to γ-radiation. On the contrary, doxorubicin, vincristine and zoledronic acid resistant cancer cells were still sensitive to radiation.

Keywords

MDR1MCF-7radioresistanceReal-time PCR
Type
Original Article
Information
Journal of Radiotherapy in Practice , Volume 8 , Issue 4 , December 2009 , pp. 207 - 213
Copyright
Copyright © Cambridge University Press 2009

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