A. Materials

N-isopropylacrylamide (NIPAAm, 98%) was purchased from Acros Organics and purified via recrystallization from hexane prior to use to remove the inhibitor. N, N′-methylenebisacrylamide (BIS, Sigma-Aldrich, St.Louis, Missouri), 2-hydroxy-2-methylpropiophenone (Daracure1173, TCI America, Portland, Oregon), ammonia persulfate (APS, Acros Organics, Somerville, New Jersey), dimethyl sulfoxide (DMSO, Fisher Chemical, Fair Lawn, New Jersey), n-octane (98%, Acros Organics, Somerville, New Jersey), SYLGARD 184 silicone elastomer kit (Krayden, Midland, Michigan), PE tubing (ID 0.015″, OD 0.043″, BD Intramedic), and double side adhesive sheet (Artgrafix, Beacon Falls, Connecticut) were used as purchased.

B. Fabrication of microfluidic device

The microfluidic channel pattern was cut in a 300 µm thick double-sided adhesive sheet using a laser cutter. The channel has a dimension of 14 mm (L) × 800 μm (W) × 300 μm (H) with two inlet holes and one outlet hole. PDMS sealing was fabricated by mixing SYLGARD 184 elastomer base and catalyst with a 10:1 ratio, the mixture was degassed and molded into 3 mm thick PDMS sheet. Inlet and outlet holes on the PDMS sealing were punctured using a biopsy with 0.8 mm diameter before device assembly. The sandwich microfluidic device was fabricated by placing the laser-cut double-sided adhesive sheet on a glass substrate followed by stacking PDMS sealing on top of the double-sided adhesive [Fig. 1(a)]. All three components were oxygen plasma treated for 2 min before assembly to improve adhesion between layers.

FIG. 1. (a) Schematic of the microfluidic device and (b) laminar flow and droplet generation inside the microfluidic device. The pre-gel solution is dyed with Rhodamine B for visual observation.

C. Synthesis of microgels

The pre-gel solution containing 20 wt% NIPAAm, 1 wt% BIS, and 1 wt% Daracure1173 in DMSO and the continuous phase n-octane were separately injected into the assembled microfluidic channel using syringe pumps (Harvard Apparatus Ph.D. Ultra & Harvard Apparatus 55-1111). These two immiscible fluids form a biphasic laminar flow through the microfluidic channel and at the location of outlet, the pre-gel solution forms droplets in the continuous n-octane phase, as discussed in detail in Sec. III. The flow rates of the two inlets were controlled separately to allow for precisely controlling and tuning the size and geometry of the produced pre-gel droplets. The pre-gel droplets were carried downstream through the outlet tubing and passed under a 40 W UV fiber lamp. Thus, the monomers in the pre-gel droplets photopolymerized and were UV-crosslinked to form solid hydrogel droplets. The produced microgels were sedimented by gravity in an ethanol bath for collection. The control sample of pNIPAAm microgel synthesized in water was fabricated following the same procedure as described above with pre-gel solution containing 20 wt% NIPAAm, 1 wt% Bis, and 1 wt% Daracure 1173 in DI water.

D. Microgel purification

The supernatant was removed from the ethanol bath to remove n-octane, DMSO, and unreacted monomers dissolved in ethanol. The microgels were then placed inside a 50 °C oven overnight to completely evaporate the residual n-octane, DMSO, and ethanol. The dried microgel samples were rehydrated with water for further characterization.

E. Synthesis of pNIPAAm hydrogels in water and DMSO

Bulk pNIPAAm hydrogels were synthesized in water and DMSO to study the solvent effect on hydrogel microstructures. 20% NIPAAm + 1% Bis + 1% APS in water and 20% NIPAAm + 1% Bis + 1% Daracure 1173 in DMSO were used as the pre-gel solution. 500 μL of pre-gel solutions were pipetted into a 2 mL glass vial, respectively. For polymerizing pNIPAAm hydrogel in water solvent, 10 μL of TEMED was injected into the vial followed by vortexing for 2 s. The gelation process finished in around 10 s. For polymerizing pNIPAAm hydrogel in DMSO, the glass vial containing pre-gel solution was exposed under a 40 W UV fiber lamp for 10 s to obtain a crosslinked gel.

F. Characterization of pNIPAAm hydrogels

The pNIPAAm hydrogels synthesized using water/DMSO as the solvent were immersed in deionized water for 48 h to remove unreacted monomers. The hydrogels were cut open to expose internal structures and freeze-dried to preserve the 3D structure of the polymer networks. The dried samples were mounted onto scanning electron microscopy stubs using conductive tape and imaged using ZEISS Supra 40VP SEM (Carl Zeiss AG, Toronto, Canada).

The morphology of pNIPAAm microgels was imaged using a Leica DMI 6000B (Leica Microsystems, Buffalo Grove, Illinois) optical microscope. Microgel solution was put inside a petri dish for characterization using transmission bright field mode of the microscope. The focal plane was carefully adjusted to show the largest cross-sectional area of the microgels.

G. Measurement of swelling ratio

The microgels synthesized using water/DMSO as the solvent were immersed in deionized water inside a petri dish for at least 48 h to reach equilibrium prior to measurement. A PID controlled heater and a temperature sensor were fixed inside the petri dish. The water temperature can be accurately maintained at the set temperature through automated on/off switching of the heater. The microgel size was recorded using an optical microscope at different bath temperatures. The volume of the microgel was calculated by assuming a spherical geometry. The swelling ratio by volume was calculated using the following formula:

$${\rm{Swelling}}\;{\rm{ratio}}\;\left( {{\rm{SR}}} \right) = {V \over {{V_0}}}\quad ,$$

where V ₀ is the equilibrium volume under room temperature and V is the volume recorded under different temperatures. For LCST measurement, the bath temperature was kept at each point for 20 min to allow the gel to reach its equilibrium volume.

For deswelling/swelling rate measurement, the pNIPAAm microgels were restricted to a small area inside the empty petri dish by spacer prior to measurement. Water with ∼50 °C temperature was poured into the petri dish to initiate deswelling. The cross-sectional size of the microgels was captured using the optical microscope with 2-s frame intervals. The swelling ratios of the microgels were calculated using the above equation. The reswelling rate for the pNIPAAm microgels were measured by first extracting the hot water in the petri dish and refill immediately with ice water. Recording process and volume conversion were same as above.

A. Pre-gel droplet formation

In conventional synthesis of microgels, surfactants are used as the particle stabilizer to prevent merging between colliding pre-gel droplets. To eliminate the use of surfactants, rapid polymerization of the pre-gel droplets must be achieved. Thus, the pre-gel droplets are fully polymerized before collision could happen, ensuring the integrity of each particle. However, rapid polymerization may not be desirable when water is used as the solvent due to the insolubility of pNIPAAm at elevated temperature. In this study, we have used DMSO as the solvent for polymerizing pNIPAAm microgels because pNIPAAm does not exhibit the volume phase transition in DMSO. Rapid polymerization of pNIPAAm microgels could be achieved by the water-free synthesis.

pNIPAAm microgels were fabricated in the microfluidic device by using DMSO as the pre-gel solution solvent. An immiscible liquid n-octane was used as the continuous phase. Photo-initiator and UV flooding were used to realize the rapid polymerization chemistry. Due to the one-step infusion of all reactants, the microfluidic device is simplified to single channel, in comparison to conventional designs where multiple channels are needed for step-wise infusion of reactants. The device simplification also benefits from the water-free chemistry. The pre-gel droplet formation mechanism is discussed below.

The Reynolds number is an important dimensionless quantity that predicts the flow pattern of liquids inside microfluidic channels. For a channel with a rectangular cross section, the Reynolds number can be calculated using the following formula:

$${\rm{Re}} = {{4Q\rho } \over {P\mu }}\quad ,$$

where Q is the volumetric flow rate of liquid, ρ is the density of liquid, P is the wetted perimeter, and µ is the dynamic viscosity of the liquid.

The pre-gel solution using DMSO as the solvent has a Reynolds number between 0.11 and 0.63 when the flow rate changes from 2 to 20 μL/min, while the continuous phase has the corresponding Reynolds number between 1.84 and 2.35. The low Reynolds number of the flowing liquids suggests the formation of laminar flow inside the microfluidic channel. This was confirmed by adding Rhodamine B as a fluorescent dye into the pre-gel solution for visual observation of the droplet generation process (Rhodamine B was not included in the actual microgel synthesis and was used here for imaging purposes only). Side-by-side biphasic laminar flow of the pre-gel solution and the continuous phase was formed inside the channel between the two immiscible liquids [Fig. 1(b)]. The biphasic laminar flow was maintained along the full length of the microfluidic channel. The interface for both liquids decreased significantly as the biphasic laminar flow entered the narrower outlet tubing. The laminar flow became less stable with decreasing wetted perimeter. The pre-gel stream was hydrodynamically focused by the continuous phase stream at the narrower outlet and broke into monodisperse droplets as the two liquids enter the outlet tubing. The formed pre-gel droplets are carried downstream by the continuous phase and photo-polymerized before collision could occur between the pre-gel droplets.

B. Size and geometry control

pNIPAAM microgels with well-controlled different sizes and geometries were successfully produced by varying the pre-gel solution (DMSO solvent) flow rate from 2 to 20 μL/min, while the continuous phase flow rate was kept at 50 μL/min. Higher flow rates were attempted but tended to lead to failure at the PDMS-double side adhesive bonded interface due to high internal pressure. The morphologies of microgels synthesized in DMSO solvent produced at different pre-gel solution flow rates are shown in Fig. 2. The produced microgels were spherical when the pre-gel solution flow rate was not higher than 3.5 μL/min [Figs. 2(a) and 2(b)], with lower pre-gel solution flow rates producing smaller particle sizes. This result is consistent with the higher local shear created at the outlet between the two phases at higher continuous phase to pre-gel solution flow rate ratio. The microgels’ shape changed from spherical to ellipsoidal then to capsule shape with the increasing flow rate of the pre-gel solution [Figs. 2(c)–2(e)]. This resulted from the outlet tubing size restriction where larger droplets were forced to elongate along the tubing direction. The axis perpendicular to the tubing was maintained at ∼400 μm for the microgels produced at pre-gel solution flow rate higher than 3.5 μL/min. Nonuniform beads composed of mixtures of microspheres, microcapsules, and long rods were produced when the flow rate for pre-gel solution exceeded 15 μL/min [Fig. 2(f)]. With more pre-gel solution flowing through the channel, a larger number of pre-gel droplets were generated in higher density and therefore had higher probability to merge with each other before being fully polymerized. The capability of easily manipulating the microgels’ geometry by simply tuning the flow rates and ratios has potential in fabricating microgels with complex structures for broad applications.

FIG. 2. Optical image of microgels fabricate using pre-gel solution flow rate from 2 μL/min (a) to 20 μL/min (f). The flow rate for n-octane was fixed at 50 μL/min.

The produced microgels showed low polydispersity. The size distribution histogram of the spherical microgels synthesized at a pre-gel solution flow rate of 2 μL/min is shown in Fig. 3. Two hundred microgel particles were randomly chosen and their diameters were measured using ImageJ. The microgels have a mean particle diameter of 304 μm and a standard deviation of 19 μm. The dispersity index was as low as 6.4%, in comparison to the relatively higher particle size variation of 10% by using other methods [Ref. Reference Sivakumaran, Mueller and Hoare31], which proves that this simple structured microfluidic device is capable of stably producing uniform microgels with low polydispersity.

FIG. 3. Size distribution of the microgels fabricated using a pre-gel solution flow rate of 2 μL/min.

C. Solvent effect on the microstructure of pNIPAAm hydrogels

Since pNIPAAm undergoes unique volume phase transition around 32 °C in aqueous environment, localized polymerization heat may lead to the insolubility or even precipitation of NIPAAm monomers out of the aqueous phase during the polymerization of pNIPAAm hydrogel. Figure 4 compares the color and the microstructures of pNIPAAm hydrogels synthesized using water [Fig. 4(a)] and DMSO [Fig. 4(b)] as a solvent. The amount of initiator was tuned so that both samples polymerized within the same short period of 10 s pNIPAAm hydrogels polymerized in water turned to white color, while hydrogels polymerized in DMSO stayed transparent. The white gel was incapable of becoming transparent again in water even after immersing in water for over 24 h. The permanent white color of the pNIPAAm hydrogels synthesized in water suggests that the formed polymer network has a microstructure that is incapable of dissolving in water, as verified by the SEM images [Fig. 4(a)].

FIG. 4. Microstructure of pNIPAAm hydrogel synthesized in water (a) and DMSO (b). The inset shows the macroscopic color of the synthesized hydrogels.

Microscopic analysis under SEM shows that the pNIPAAm hydrogels synthesized in water has agglomerated polymer network with pore size around 1–5 μm. By contrast, pNIPAAm hydrogels synthesized in DMSO show more uniform microstructure with submicron pores. The agglomerates in pNIPAAm hydrogels synthesized in water may result from the hydrophobic aggregation of pNIPAAm networks when the polymerization heat is generated fast enough to raise the sample temperature above the LCST of pNIPAAm. The formed agglomerates were difficult to dissolve in water, which corresponds to the macroscopically unrecoverable white gel. Therefore, synthesizing pNIPAAm hydrogels in water tends to produce agglomerated polymers that has minimum interaction with water, which may lead to low thermosensitivity, reduced swelling ratio, and a more gradual volume phase transition. Using organic solvents such as DMSO to replace water is more advantageous because it not only accommodates rapid polymerization that allows for a surfactant-free synthesis of pNIPAAm hydrogels but also maintained or even enhanced hydrogel quality.

D. Thermally responsive behaviors of rapidly polymerized pNIPAAm microgels

The swelling ratios of the pNIPAAm microgels synthesized in water and DMSO have been studied as shown in Fig. 5. The insets in Fig. 5 are the corresponding optical image of microgels under 26 and 38 °C. Here, we use the microgels fabricated under a pre-gel solution flow rate of 2 μL/min because the spherical microgel deforms isotopically in all directions and the particle volume can be accurately converted from the cross-sectional area captured under the optical microscope. The microgels produced in DMSO show a large volume reduction of 64% when temperature increased from 25 to 38 °C. By contrast, the microgels produced in water have much smaller volume reduction of only 28%, which confirms that with an aggregated polymer network, the swelling ratio of pNIPAAm microgels synthesized in water significantly decreased, which defeats the intrinsic thermoresponsive functions of pNIPAAm. Additionally, 90% of the total volume reduction took place between 30 and 33 °C for the microgels produced in DMSO. The LCST of microgels produced in DMSO is the mid-point 31.5 °C, which is in accordance with the known volume phase transition temperature around 32 °C for pNIPAAm. The LCST of the produced microgels was not affected by using DMSO as a solvent and the rapid synthesis. By contrast, the 90% volume reduction temperature range expanded to between 28 and 33 °C for the microgels produced in water. This result suggests that the known sharp volume phase transition behavior becomes more gradual when pNIPAAm is rapidly polymerized in water.

FIG. 5. Swelling ratio versus temperature curve of the microgels fabricated using a pre-gel solution flow rate of 2 μL/min.

The response rate of the pNIPAAm microgels were characterized by plotting the swelling ratio versus time curve. Figure 6(a) shows the deswelling rate of the microgels. The volume of microgels reduced exponentially after immersing in 50 °C water for 70 s. The microgels produced in DMSO shows steeper slope in comparison with the microgels produced in water. For achieving the same degree of shrinking from to 80% of the original volume, microgels produced in DMSO used 8 s while microgels produced in water used 65 s. More than 8 times faster deswelling rate was achieved. The reswelling rate of the same particle is shown in Fig. 6(b), faster swelling rate of the microgels produced in DMSO was also observed in comparison to relatively slower reswelling, and hysteresis observed in the microgels produced in water. This further indicates the high quality and good performance of the microgels produced by our method. The total volume reduction captured during the 70-s rapid deswelling was smaller than the volume reduction at the equilibrium value. This could be explained by the continuously reduced pore size during shrinking, which slowed down the water diffusion out of the gel. The reswelling rates were 8–10 times slower than deswelling and the microgel particles recovered to their original volume after 200 s. The slower reswell process was in accordance with the trend seen in most osmosis gels. Overall, the pNIPAAm microgels synthesized in DMSO well maintained the desirable thermal response rate, which was comparable to the standard swelling/deswelling kinetics of pNIPAAm.^{Reference Gan, Guan and Zhang39,Reference Xing, Guan and Zhang40} The exhibited fast thermal responsiveness and large volume change ratio of our pNIPAAm microgels successfully eliminated the compromised thermal volume changing issue associated with the conventionally fabrication methods.

FIG. 6. Deswelling (a) and swelling (b) kinetics of pNIPAAm microgels fabricated using a pre-gel solution flow rate of 2 μL/min.