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Nano and micro scale analysis of dentin with in vitro and high speed atomic force microscopy

Published online by Cambridge University Press:  17 June 2013

Yasemin Kutes ,
Varun Vyas  and
Bryan D. Huey
Yasemin Kutes
Affiliation:
Department of Materials Science & Engineering, University of Connecticut, Storrs, Connecticut 06269-3136
Varun Vyas
Affiliation:
Department of Materials Science & Engineering, University of Connecticut, Storrs, Connecticut 06269-3136
Bryan D. Huey*
Affiliation:
Department of Materials Science & Engineering, University of Connecticut, Storrs, Connecticut 06269-3136
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

Atomic force microscopy (AFM) has proven useful in the investigation of porous surfaces due to its nanoscale spatial resolution, micron scale range, compatibility with nonconducting materials, and even applicability ability to biological systems since it can operate in fluids. Since AFM directly measures the surface by contact, it is particularly suited for quantifying the roughness, and more appropriately for porous and particulate materials, the surface area. In this work, a multi-scale porous material, human molar dentin, was studied with AC mode AFM (both in-air and in-liquid), enabling extensive analyses both for plain dentin as well as specimens exposed to nanoparticle TiO2 containing toothpaste to approximate personal dental hygiene. Finally, high speed AFM is also demonstrated in vitro with equivalent results, except that the time required per image is reduced by several orders of magnitude from tens of minutes to as little as 6 s. Careful implementation of AFM, both at standard and high speeds, is therefore effective for investigating highly porous materials, including biological tissue, in environmentally or physiologically relevant conditions.

Type
Articles
Information
Journal of Materials Research , Volume 28 , Issue 17: Focus Issue: Advances in the Synthesis, Characterization, and Properties of Bulk Porous Materials , 14 September 2013 , pp. 2300 - 2307
Copyright
Copyright © Materials Research Society 2013 

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References

REFERENCES

Binnig, G., Quate, C.F., and Gerber, C.: Atomic force microscope. Phys. Rev. Lett. 56, 930–933 (1986).CrossRefGoogle ScholarPubMed
Bonnell, D.: Scanning Probe Microscopy and Spectroscopy Theory, Techniques, and Applications, 2nd ed. (Wiley-VCH, New York, 2001).Google Scholar
Ulfig, R.M., Pangrle, S.K., Myers, A.F., and Romero, J.D.: Method for determining pore characteristics in porous materials. U.S. Patent No. 6,791,081. Sep. 14, 2004. Washington, DC: U.S. Patent and Trademark Office.Google Scholar
Stevens, S.M., Loiola, A.R., Cubillas, P., da Silva, L.R.D., Terasaki, O., and Anderson, M.W.: Hierarchical porous materials: Internal structure revealed by argon ion-beam cross-section polishing, HRSEM and AFM. Solid State Sci. 13, 745–749 (2011).CrossRefGoogle Scholar
Park, S.I., Chung, S.H., and Byoung-Woon, A.H.N.: Scanning probe microscope capable of measuring samples having overhang structure. U.S. Patent Application 12/773, 649 (2008).Google Scholar
Cho, S.J., Ahn, B.W., Kim, J., Lee, J.M., Hua, Y., Yoo, Y.K., and Park, S.: Three-dimensional imaging of undercut and sidewall structures by atomic force microscopy. Rev. Sci. Instrum. 82, 023707 (2011).CrossRefGoogle ScholarPubMed
Hua, Y., Coggins, C., and Park, S.: Advanced 3D metrology atomic force microscope, in Advanced Semiconductor Manufacturing Conference (ASMC), 2010 IEEE/SEMI, July 11–13, 2010 (IEEE), pp. 7–10.CrossRefGoogle Scholar
Nath, R., Chu, Y-H., Polomoff, N.A., Ramesh, R., and Huey, B.D.: High speed piezoresponse force microscopy: <1 frame per second nanoscale imaging. Appl. Phys. Lett. 93, 072905–072905-3 (2008).CrossRefGoogle Scholar
Huey, B.D.: AFM and acoustics: Fast, quantitative nanomechanical mapping. Annu. Rev. Mater. Res. 37, 351–385 (2007).CrossRefGoogle Scholar
Huey, B.D., Premnath, R.N., Lee, S., and Polomoff, N.A.: High speed SPM applied for direct nanoscale mapping of the influence of defects on ferroelectric switching dynamics. J. Am. Ceram. Soc. 95, 1147–1162 (2012) <Go to ISI>://WOS:000302401600001.CrossRefGoogle Scholar
Bosse, J.L.: High speed atomic force microscopy techniques for the efficient study of nanotribology. Master’s Theses. Storrs, CT, Paper 260, 2012 http://digitalcommons.uconn.edu/gs_theses/260.Google Scholar
Ando, T., Kodera, N., Takai, E., Maruyama, D., Saito, K., and Toda, A.: A high-speed atomic force microscope for studying biological macromolecules. PNAS 98, 12468–12472 (2001) <Go to ISI>://000171806100034.CrossRefGoogle ScholarPubMed
Humphris, A.D.L., Miles, M.J., and Hobbs, J.K.: A mechanical microscope: High-speed atomic force microscopy. Appl. Phys. Lett. 86(3), 034106 (2005) <Go to ISI>://000226864600094.CrossRefGoogle Scholar
Pyne, A., Marks, W., Picco, L., Dunton, P.G., Ulcinas, A., Barbour, M.E., Jones, S.B., Gimzewski, J., and Miles, M.J.: High-speed atomic force microscopy of dental enamel dissolution in citric acid. Arch. Histol. Cytol. 72(4/5), 209–215 (2009).CrossRefGoogle ScholarPubMed
Bertassoni, L.E., Habelitz, S., Pugach, M., Soares, P.C., Marshall, S.J., and Marshall, G.W. Jr.: Evaluation of surface structural and mechanical changes following remineralization of dentin. Scanning 32, 312–319 (2010).CrossRefGoogle ScholarPubMed
Petrou, I., Heu, R., Stranick, M., Lavender, S., Zaidel, L., Cummins, D., Sullivan, R.J., Hsueh, C., and Gimzewski, J.K.: A breakthrough therapy for dentin hypersensitivity: How dental products containing 8% arginine and calcium carbonate work to deliver effective relief of sensitive teeth. J. Clin. Dent. 20, 23–31 (2009).Google ScholarPubMed
Silikas, N., Watts, D.C., England, K.E., and Jandt, K.D.: Surface fine structure of treated dentine investigated with tapping mode atomic force microscopy (TMAFM). J. Dent. 27, 137–144 (1999).CrossRefGoogle ScholarPubMed
Kubinek, R., Zapletalova, Z., Vujtek, M., Novotny, R., Kolarova, H., Chmelickova, H., and Perina, J. Jr.: Sealing of open dentinal tubules by laser irradiation: AFM and SEM observations of dentine surfaces. J. Mol. Recognit. 20, 476–482 (2007).CrossRefGoogle ScholarPubMed
Zapletalova, Z., Kubinek, R., Vujtek, M., and Novotny, R.: Examination of dentin surface using AFM (our experience). Acta Medica (Hradec Kralove), 47, 343–346 (2004).CrossRefGoogle ScholarPubMed
Sharma, S., Cross, S.E., Hsueh, C., Wali, R.P., Stieg, A.Z., and Gimzewski, J.K.: Nanocharacterization in dentistry. Int. J. Mol. Sci. 11, 2523–2545 (2010).CrossRefGoogle ScholarPubMed
Mello, S.V., Arvanitidou, E., Stranick, M.A., Santana, R., Kutes, Y., and Huey, B.: Mode of action studies of a new desensitizing mouthwash containing 0.8% arginine, PVM/MA copolymer, pyrophosphates, and 0.05% sodium fluoride. J. Dent. 41S, S12–S19 (2013).CrossRefGoogle Scholar
Asylum Research. iDrive™ Magnetic Actuated Cantilever. (2013). Available from: http://www.asylumresearch.com/Products/iDrive/iDrive.shtml.Google Scholar
Austin, M., Herbert, A., Black, R., and Riches, P.: Atomic force microscopy of bovine articular cartilage, in Proceedings of the 6th UKRI PG Conference in Biomedical Engineering and Medical Physics 2011 PGBIOMED 2011, edited by A. Syed and C. Hamilton, p. 17.Google Scholar
George, P.A., Donose, B.C., and Cooper-White, J.J.: Self-assembling polystyrene-block-poly (ethylene oxide) copolymer surface coatings: Resistance to protein and cell adhesion. Biomaterials 30, 2449–2456 (2009).CrossRefGoogle ScholarPubMed
Picco, L.M., Bozec, L., Ulcinas, A., Engledew, D.J., Antognozzi, M., Horton, M.A., and Miles, M.J.: Breaking the speed limit with atomic force microscopy. Nanotechnology 18, 1–4 (2007).CrossRefGoogle Scholar
Wallace, J.M., Chen, Q., Fang, M., Erickson, B., Orr, B.G., and Banaszak Holl, M.M.: Type I collagen exists as a distribution of nanoscale morphologies in teeth, bones, and tendons. Langmuir 26, 7349–7354 (2010).CrossRefGoogle ScholarPubMed
Kutes, Y.: Nanocharacterization of Porous Materials with Atomic Force Microscopy. Master's Theses. Storrs, CT, Paper 262http://digitalcommons.uconn.edu/gs_theses/262.Google Scholar
Palumbo, V.P., Kovalskiy, A., Jain, H., and Huey, B.D.: Direct investigation of silver photodissolution dynamics and reversibility in arsenic trisulphide thin films by atomic force microscopy. Nanotechnology 24(12), 125706 (2013) http://stacks.iop.org/0957-4484/24/125706.CrossRefGoogle ScholarPubMed
Addy, M. and Shellis, R.: Interaction between attrition, abrasion and erosion in tooth wear. Monogr. Oral Sci. 20, 17–31 (2006).CrossRefGoogle Scholar