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Investigation of the viscoelasticity of human osteosarcoma cells using a shear assay method

Published online by Cambridge University Press:  01 August 2006

Yifang Cao ,
Randy Bly ,
Will Moore ,
Zhan Gao ,
Alberto M. Cuitino  and
Wole Soboyejo
Yifang Cao
Affiliation:
Princeton Institute for the Science and Technology of Materials (PRISM) and Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, New Jersey 08544
Randy Bly
Affiliation:
Princeton Institute for the Science and Technology of Materials (PRISM) and Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, New Jersey 08544
Will Moore
Affiliation:
Princeton Institute for the Science and Technology of Materials (PRISM) and Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, New Jersey 08544
Zhan Gao
Affiliation:
Department of Mechanical and Aerospace Engineering, Rutgers University, Piscataway, New Jersey 08854
Alberto M. Cuitino
Affiliation:
Department of Mechanical and Aerospace Engineering, Rutgers University, Piscataway, New Jersey 08854
Wole Soboyejo*
Affiliation:
Princeton Institute for the Scienceand Technology of Materials (PRISM)and Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, New Jersey 08544
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

This paper presents a shear assay method for the determination of the viscoelastic properties of biological cells. The method was applied to the measurement of the viscoelastic properties of human osteosarcoma (HOS) cells. It involves a combination of shear assay experiments and digital image correlation techniques. Following in situ observations of cell deformation during shear assay experiments, a digital image correlation (DIC) technique was used to determine the local displacement and strain fields. The creep curves were also extracted from multiple digital images that were used to extract the time dependence of local strain under constant stress conditions. The measured creep curves were well described by a generalized viscoelastic Maxwell model. The extracted elastic and viscous parameters were in good agreement with results obtained from prior studies with other techniques. The results also suggested that the nucleus is stiffer than the surrounding cytoplasm of HOS cells.

Keywords

CellularElastic propertiesFludics
Type
Articles
Information
Journal of Materials Research , Volume 21 , Issue 8 , August 2006 , pp. 1922 - 1930
Copyright
Copyright © Materials Research Society 2006

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