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Indentation micromechanics of three-dimensional fibrin/collagen biomaterial scaffolds

Published online by Cambridge University Press:  01 August 2006

R.G. Mooney ,
C.A. Costales ,
E.G. Freeman ,
J.M. Curtin ,
A.A. Corrin ,
J.T. Lee ,
S. Reynolds ,
B. Tawil  and
M.C. Shaw
R.G. Mooney
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
C.A. Costales
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
E.G. Freeman
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
J.M. Curtin
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
A.A. Corrin
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
J.T. Lee
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
S. Reynolds
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
B. Tawil
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
M.C. Shaw*
Affiliation:
Bioengineering, California Lutheran University, Thousand Oaks, California 91360
*
a) Address all correspondence to this author. e-mail: [email protected]
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Abstract

The underlying relationships between the microstructure and time-dependent mechanical properties of hydrated fibrin, collagen, and fibrin/collagen composite materials have been explored using an adaptation of the classical rigid, cylindrical, flat punch loaded normally to a planar specimen surface. A suite of quasi-static elastic and viscoelastic indentation experiments have been conducted with uniformly mixed fibrin, collagen, and fibrin/collagen composites, in addition to macrolayered collagen materials. Coupled with insights obtained from optical and confocal fluorescence microscopy, a simple micromechanics model has been developed for the effect of local microstructural variables on the macroscopic mechanical stiffness. These results demonstrate the efficacy of this technique to efficiently and reproducibly probe hydrated engineered tissue replacement materials for local variations in viscoelastic material behavior without the need for extensive specimen preparation or grips, as well as being suitable for performing directly comparable measurements with explants of human skin.

Keywords

BiomedicalElastic propertiesMicrostructure
Type
Articles
Information
Journal of Materials Research , Volume 21 , Issue 8 , August 2006 , pp. 2023 - 2034
Copyright
Copyright © Materials Research Society 2006

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References

REFERENCES

1.Clark, R.: The Molecular and Cellular Biology of Wound Repair, 2nd ed. (Plenum Press, New York, 1996).Google Scholar
2.Tawil, B. Fibrin and its applications, in An Introduction to Biomaterials. (2006, in press).Google Scholar
3.Weisel, J.: The mechanical properties of fibrin for basic scientists and clinicians. Biophys. Chem. 112, 267 (2004).CrossRefGoogle ScholarPubMed
4.Currie, L.J., Sharpe, J.R., Marti, R.: The use of fibrin glue in skin grafts and tissue-engineered skin replacements: A review. Plast. Reconstr. Surg. 108, 1713 (2001).CrossRefGoogle ScholarPubMed
5.Helgerson, S., Seelich, T., Di Orio, J., Tawil, B., Bittner, K., Spaethe, R. Fibrin. Review, in Encyclopedia of Biomaterials and Biomedical Engineering edited by Bowlin, G., Wnek, G. (Marcel Dekker, New York, 2004).Google Scholar
6.Ferry, J.D., Morrison, P.R.: Preparation and properties of serum and plasma proteins. VIII. The conversion of human fibrinogen to fibrin under various conditions. J. Am. Chem. Soc. 388(1947).Google Scholar
7.Henry, F., Nestler, M.: A physical model for a fibrous network and its application to the shear modulus and other data of the fibrin gel. Biophys. Chem. 112, 181 (2004).CrossRefGoogle Scholar
8.Danielsson, M., Parks, D.M., Boyce, M.C.: Constitutive modeling of porous hyperelastic materials. Mech. Mater. 36, 347 (2004).CrossRefGoogle Scholar
9.Meredith, J.E., Fazeli, B., Schwartz, M.A.: The extracellular matrix as a cell survival factor. Mol. Biol. Cell 4, 953 (1993).CrossRefGoogle ScholarPubMed
10.Roberts, W.W., Kramer, O., Rosser, R.W., Nestler, F.H.M., Ferry, J.D.: Rheology of fibrin clots. I. Dynamic viscoelastic properties and fluid permeation. Biophys. Chem. 1, 152 (1974).CrossRefGoogle ScholarPubMed
11.Fung, Y.C.: Biomechanics: Mechanical Properties of Living Tissues, 2nd ed. (Springer, New York, 1993).CrossRefGoogle Scholar
12.Lakes, R.S.: Viscoelastic measurement techniques: Review article: Viscoelastic measurement techniques. Rev. Sci. Instrum. 75(4), 4 (2004).CrossRefGoogle Scholar
13.Han, L., Noble, J.A., Burcher, M.: A novel ultrasound indentation system for measuring biomechanical properties of in vivo soft tissue. Ultrasound Med. Biol. 29(6), 813 (2003).CrossRefGoogle ScholarPubMed
14.Janmey, P.: A. torsion pendulum for measurement of the viscoelasticity of biopolymers and its application to actin networks. J. Biochem. Biophys. Methods 22, 41 (1999).CrossRefGoogle Scholar
15.Discher, D.E., Janmey, P., Wang, Y-L.: Tissue cells feel and respond to the stiffness of their substrate. Science 310, 18 (2005).CrossRefGoogle Scholar
16.Mooney, R.G., Costales, C.A., Curtin, J.M., Tawil, B., and Shaw, M.C.: Indentation micromechanics of fibroblast-populated fibrin constructs, in Structure and Mechanical Behavior of Biological Materials edited by Fratzl, P., Landis, W.J., Wang, R., and Silver, F.H. (Mater. Res. Soc. Symp. Proc. 874 Warrendale, PA, 2005), L7.10, p. 205.Google Scholar
17.Hill, R.: Theory of Plasticity (Oxford University Press, Oxford, UK, 1954).Google Scholar
18.Sneddon, I.N.: The relation between load and penetration in the axisymmetric Boussinesq problems for a punch of arbitrary profile. Int. J. Eng. Sci. 3, 47 (1965).CrossRefGoogle Scholar
19.Loubet, J., Georges, J., Meille, G. Vickers indentation curves of elastoplastic materials, in Microindentation Techniques in Materials Science and Engineering, edited by Blau, P. and Lawn, B.R. (ASTM STP 889, 1984).CrossRefGoogle Scholar
20.Lamethe, J.F., Sergot, P., Chateauminois, A., Briscoe, B.J.: Contact fatigue behaviour of glassy polymers with improved toughness under fretting wear conditions. Wear 255, 758 (2003).CrossRefGoogle Scholar
21.Bonne, M., Briscoe, B.J., Manimaaran, S., Allen, A.: Characterization of surface abrasion phenomena on poly(methyl methacrylate) surfaces. Wear 254, 55 (2003).CrossRefGoogle Scholar
22.Oliver, W.C., Hutchings, R., Pethica, J.B. Measurement of hardness at indentation depths as low as 20 nanometres, in Microindentation Techniques in Materials Science and Engineering, edited by Blau, P. and Lawn, B.R. (ASTM STP 889, 1984).CrossRefGoogle Scholar
23.Fan, Z., Swadener, J.G., Rho, J.Y., Roy, M.E., Pharr, G.M.: Anisotropic properties of human tibial cortical bone as measured by nanoindentation. J. Orthop. Res. 20, 806 (2002).CrossRefGoogle ScholarPubMed
24.Rho, J.Y., Zioupos, P., Currey, J.D., Pharr, G.M.: Microstructural elasticity and regional heterogeneity in human femoral bone of various ages examined by nano-indentation. J. Biomechs. 35, 189 (2002).CrossRefGoogle ScholarPubMed
25.Silva, M.J., Bridt, M.D., Fan, A., Rho, J-Y.: Nanoindentation and whole-bone bending estimates of material properties in bones from the senescence accelerated mouse SAMP6. J. Biomechs. 37, 1639 (2004).CrossRefGoogle ScholarPubMed
26.Korhonen, R.K., Wong, M., Arokoski, J., Lindgren, R., Helminen, H.J., Hunziker, E.B., Jurvelin, J.S.: Importance of the superficial tissue layer for the indentation stiffness of articular cartilage. Med. Eng. Phys. 24, 99 (2002).CrossRefGoogle ScholarPubMed
27.Qin, Q-H., Swain, M.V.: A micro-mechanics model of dentin mechanical properties. Biomaterials 25, 5081 (2004).CrossRefGoogle ScholarPubMed
28.Shergold, O.A., Fleck, N.A.: Mechanisms of deep penetration of soft solids. Proc. R. Soc. London A 460, 3037 (2004).CrossRefGoogle Scholar
29.Gambarotta, L., Massabo, R., and Morbiducci, R.: Mechanical characterization of human skin from in vivo tests and simulation of reconstructive surgery. Proceedings of International Mechanical Engineering Congress and Exposition Washington, DC, 2003.Google Scholar
30.Gambarotta, L., Massabo, R., Morbiducci, R.: A finite element model for characterizing the mechanical properties of human skin using in vivo data. J. Plast. Reconstruct. Surg. 1(2003).Google Scholar
31.Tawil, B. Fibrin and its characteristics. Wound Repair and Regeneration Newsletter, November 2, 2003.Google Scholar
32.Kadler, K.E., Holmes, D.F., Trotter, J.A., Chapman, J.A.: Collagen fibril formation. J. Biochem. (Tokyo) 316, 1 (1996).CrossRefGoogle ScholarPubMed
33.Shigley, J.E.: Mechanical Engineering Design (McGraw-Hill, New York, 1983).Google Scholar
34.Atala, A.: Methods of Tissue Engineering, 2002 (Academic Press, Elsevier, San Diego, CA, 2002).Google Scholar
35.Cox, S., Marietta-Cole, M., Tawil, B.: Behavior of human dermal fibroblasts in three-dimensional fibrin clots: Dependence on fibrinogen and thrombin concentration. Tissue Eng. 10, 942 (2004).CrossRefGoogle ScholarPubMed
36.Clyne, T.W.: An Introduction to Metal Matrix Composites. (Cambridge University Press, Cambridge, UK, 1993).CrossRefGoogle Scholar
37.Corrin, A.A., Freeman, E.G., Lee, J.T., Tawil, B., and Shaw, M.C.: unpublished.Google Scholar