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In vitro mineralization on chitosan using solutions with excess of calcium and phosphate ions

Published online by Cambridge University Press:  01 December 2005

Marisa Masumi Beppu ,
Marco Antonio Torres ,
Cassiano Gomes Aimoli ,
Gilberto Alessandre Soares Goulart  and
Cesar Costapinto Santana
Marisa Masumi Beppu*
Affiliation:
Faculdade de Engenharia Química, Unicamp CP6066 CEP13083-970, Campinas, Sao Paulo, Brazil
Marco Antonio Torres
Affiliation:
Faculdade de Engenharia Química, Unicamp CP6066 CEP13083-970, Campinas, Sao Paulo, Brazil
Cassiano Gomes Aimoli
Affiliation:
Faculdade de Engenharia Química, Unicamp CP6066 CEP13083-970, Campinas, Sao Paulo, Brazil
Gilberto Alessandre Soares Goulart
Affiliation:
Faculdade de Engenharia Química, Unicamp CP6066 CEP13083-970, Campinas, Sao Paulo, Brazil
Cesar Costapinto Santana
Affiliation:
Faculdade de Engenharia Química, Unicamp CP6066 CEP13083-970, Campinas, Sao Paulo, Brazil
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

Pseudo-simulated body fluids (SBFs) were used in in vitro experiments to promote chitosan porous membrane calcification. Common SBFs, which had concentrations of phosphate or calcium ions doubled, were so named because they do not replicate, by rigor, a genuine body fluid ion concentration. The objective of using such calcification fluids was to study the influence of phosphate and calcium excess in solution on mineralization deposit characteristics. SEM-EDS analyses showed that morphology and composition of deposits varies depending on which ion (phosphate or calcium) is in excess; x-ray diffractograms show that deposits are poorly crystalline (like biological apatites) but still show better crystallinity in deposits generated from P-rich SBF. This result, added to previous ones [such as those reported by Beppu and Santana Mater. Res.5, 47 (2002)] where a difference in the interconnectivity of the inorganic and organic (matrix) phases was stressed, suggests different deposition processes for each situation.

Keywords

BiomedicalBiomimeticCoating
Type
Articles
Information
Journal of Materials Research , Volume 20 , Issue 12 , December 2005 , pp. 3303 - 3311
Copyright
Copyright © Materials Research Society 2005

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