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Antithrombotic, antimicrobial activities, and biocompatibility of surface-functionalized titanium

Published online by Cambridge University Press:  05 December 2019

Guannan Zhang ,
Xingyu Zhang ,
Zhiping Sun ,
Xiaohong Yao  and
Xiangyu Zhang
Guannan Zhang*
Affiliation:
College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, People’s Republic of China
Xingyu Zhang*
Affiliation:
College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, People’s Republic of China
Zhiping Sun
Affiliation:
School of Electromechanical and Automobile Engineering, Changzhou Vocational Institute of Engineering, Changzhou 213164, People’s Republic of China
Xiaohong Yao*
Affiliation:
College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, People’s Republic of China
Xiangyu Zhang*
Affiliation:
College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, People’s Republic of China
*
a)Address all correspondence to these authors. e-mail: [email protected]
b)e-mail: [email protected]
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Abstract

Titanium (Ti) has been extensively used in medical devices owing to their low density, high strength, excellent corrosion resistance, and biocompatibility. However, thrombus formation and bacterial infections are still challenges for their application in specific clinical cases. Hence, we have developed a simple, efficient, and stable strategy that endow Ti with anticoagulant and antibacterial properties through chemical bonding and electrostatic bonding. A large number of hydroxyl groups were produced on the surface of Ti by annealing at 500 °C. Then, heparin was immobilized on annealed surface with chemical bonding and chitosan was captured in an electrostatically bound manner by simply soaking in solution. The results indicated that the surface-functionalized Ti exhibited excellent anticoagulant properties by a reduction in platelet adhesion and prolonged blood clotting time. Furthermore, the modified Ti also showed antibacterial properties against both Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus).

Keywords

Tiannealingantithrombogenic activitiesantibacterial activitiesbiomaterial
Type
Article
Information
Journal of Materials Research , Volume 34 , Issue 24 , 30 December 2019 , pp. 4056 - 4065
Copyright
Copyright © Materials Research Society 2019 

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References

Hasan, J., Crawford, R.J., and Ivanova, E.P.: Antibacterial surfaces: The quest for a new generation of biomaterials. Trends Biotechnol. 31, 295 (2013).CrossRefGoogle ScholarPubMed
de los Santos Pereira, A., Sheikh, S., Blaszykowski, C., Pop-Georgievski, O., Fedorov, K., Thompson, M., and Rodriguez-Emmenegger, C.: Antifouling polymer brushes displaying antithrombogenic surface properties. Biomacromolecules 17, 1179 (2016).CrossRefGoogle ScholarPubMed
Murugesan, S., Xie, J., and Linhardt, R.J.: Immobilization of heparin: Approaches and applications. Curr. Top. Med. Chem. 8, 80 (2008).Google ScholarPubMed
Goddard, J.M. and Hotchkiss, J.H.: Polymer surface modification for the attachment of bioactive compounds. Prog. Polym. Sci. 32, 698 (2007).CrossRefGoogle Scholar
Masters, K.S.: Covalent growth factor immobilization strategies for tissue repair and regeneration. Macromol. Biosci. 11, 1149 (2011).CrossRefGoogle ScholarPubMed
Lee, H., Rho, J., and Messersmith, P.B.: Facile conjugation of biomolecules onto surfaces via mussel adhesive protein inspired coatings. Adv. Mater. 21, 431 (2009).CrossRefGoogle ScholarPubMed
Aydin, D. and Kizilel, S.: P2X7 receptor antagonist delivery vehicle based on photocrosslinked amphiphilic hybrid gels. RSC Adv. 8, 18216 (2018).CrossRefGoogle Scholar
Wu, S., Weng, Z., Liu, X., Yeung, K.W.K., and Chu, P.K.: Functionalized TiO2 based nanomaterials for biomedical applications. Adv. Funct. Mater. 24, 5464 (2015).CrossRefGoogle Scholar
Dinan, B., Gallego-Perez, D., Lee, H., Hansford, D., and Akbar, S.A.: Thermally grown TiO2, nanowires to improve cell growth and proliferation on titanium based materials. Ceram. Int. 39, 5949 (2013).CrossRefGoogle Scholar
Geetha, M., Singh, A.K., Asokamani, R., and Gogia, A.K.: Ti based biomaterials, the ultimate choice for orthopaedic implants—A review. Prog. Mater. Sci. 54, 397 (2009).CrossRefGoogle Scholar
Wu, S., Liu, X., Yeung, K.W.K., Liu, C., and Yang, X.: Biomimetic porous scaffolds for bone tissue engineering. Mater. Sci. Eng., R 80, 1 (2014).CrossRefGoogle Scholar
He, X.J., Zhang, G.N., Wang, X., Hang, R.Q., Huang, X.B., Qin, L., Tang, B., and Zhang, X.Y.: Biocompatibility, corrosion resistance and antibacterial activity of TiO2/CuO coating on titanium. Ceram. Int. 43, 16185 (2017).CrossRefGoogle Scholar
Ghosh, A.: Effect of rolling mode and annealing temperature on microstructure and texture of commercially pure-titanium. Mater. Charact. 125, 83 (2017).CrossRefGoogle Scholar
Rendón-Rivera, A., Toledo-Antonio, J.A., Cortés-Jácome, M.A., and Angeles-Chávez, C.: Generation of highly reactive OH groups at the surface of TiO2, nanotubes. Catal. Today 166, 18 (2011).CrossRefGoogle Scholar
Liu, D.W., Xiao, P., Zhang, Y.H., Garcia, B.B., Zhang, Q.F., Guo, Q., Champion, R., and Cao, G.Z.: TiO2 nanotube arrays annealed in N2 for efficient lithium-ion intercalation. J. Phys. Chem. C 112, 11175 (2008).CrossRefGoogle Scholar
Fujino, T., Katayama, M., Inudzuka, K., Okuno, T., and Oura, K.: Surface hydroxyl formation on vacuum-annealed TiO2(110) TiO2(110). Appl. Phys. Lett. 79, 2716 (2001).CrossRefGoogle Scholar
Arrouvel, C., Digne, M., Breysse, M., Toulhoat, H., and Raybaud, P.: Effects of morphology on surface hydroxyl concentration: A DFT comparison of anatase–TiO2, and γ-alumina catalytic supports. J. Catal. 222, 152 (2004).CrossRefGoogle Scholar
Sie, P., Ofosu, F., Fernandez, F., Buchanan, M.R., Petitou, M., and Boneu, B.: Respective role of antithrombin III and heparin cofactor II in the in vitro anticoagulant effect of heparin and of various sulphated polysaccharides. Br. J. Haematol. 64, 707 (1986).CrossRefGoogle ScholarPubMed
Rosenberg, R.D. and Damus, P.S.: The purification and mechanism of action of human antithrombin-heparin cofactor. J. Biol. Chem. 248, 6490 (1973).Google ScholarPubMed
Salzman, E.W., Rosenberg, R.D., Smith, M.H., Lindon, J.N., and Favreau, L.: Effect of heparin and heparin fractions on platelet aggregation. J. Clin. Invest. 65, 64 (1980).CrossRefGoogle ScholarPubMed
Muzzarelli, R.A.: Biomedical exploitation of chitin and chitosan via mechano-chemical disassembly, electrospinning, dissolution in imidazolium ionic liquids, and supercritical drying. Mar. Drugs 9, 1510 (2011).CrossRefGoogle ScholarPubMed
Hu, L., Sun, Y., and Wu, Y.: Advances in chitosan-based drug delivery vehicles. Nanoscale 5, 3103 (2013).CrossRefGoogle ScholarPubMed
Benhabiles, M.S., Salah, R., Lounici, H., Drouiche, N., Mfa, G., and Mameri, N.: Antibacterial activity of chitin, chitosan and its oligomers prepared from shrimp shell waste. Food Hydrocolloids 29, 48 (2012).CrossRefGoogle Scholar
Rabea, E.I., Badawy, M.E., Stevens, C.V., Smagghe, G., and Steurbaut, W.: Chitosan as antimicrobial agent: Applications and mode of action. Biomacromolecules 4, 1457 (2003).CrossRefGoogle ScholarPubMed
Upadhyaya, L., Singh, J., Agarwal, V., and Tewari, R.P.: The implications of recent advances in carboxymethyl chitosan based targeted drug delivery and tissue engineering applications. J. Controlled Release 186, 54 (2014).CrossRefGoogle ScholarPubMed
Raftery, R., O’Brien, F.J., and Cryan, S.A.: Chitosan for gene delivery and orthopedic tissue engineering applications. Molecules 18, 5611 (2013).CrossRefGoogle ScholarPubMed
Asharani, P.V., Mun, G.L.K., Hande, M.P., and Valiyaveettil, S.: Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano 85, 743 (2011).Google Scholar
Piret, J.P., Jacques, D., Audinot, J.N., Mejia, J., Boilan, E., Noël, F., Fransolet, M., Demazy, C., Lucas, S., Saout, C., and Toussaint, O.: Copper(II) oxide nanoparticles penetrate into HepG2 cells, exert cytotoxicity via oxidative stress and induce pro-inflammatory response. Nanoscale 4, 7168 (2012).CrossRefGoogle ScholarPubMed
Antonio, A.S., Wiedemann, L.S.M., and Junior, V.F.V.: The genus capsicum: A phytochemical review of bioactive secondary metabolite. RSC Adv. 8, 25767 (2018).CrossRefGoogle Scholar
Xu, F.J., Li, Y.L., Kang, E.T., and Neoh, K.G.: Heparin-coupled poly(poly(ethylene glycol) monomethacrylate)–Si(111) hybrids and their blood compatible surfaces. Biomacromolecules 6, 1759 (2005).CrossRefGoogle ScholarPubMed
Cheng, C., Sun, S., and Zhao, C.: Progress in heparin and heparin-like/mimicking polymer-functionalized biomedical membranes. J. Mater. Chem. B 2, 7649 (2014).CrossRefGoogle Scholar
Le Thi, P., Lee, Y., Kwon, H.J., Park, K.M., Lee, M.H., Park, J-C., and Park, K.D.: Tyrosinase-mediated surface coimmobilization of heparin and silver nanoparticles for antithrombotic and antimicrobial activities. ACS Appl. Mater. Interfaces 9, 20376 (2017).CrossRefGoogle ScholarPubMed
Sivaraman, B. and Latour, R.A.: The relationship between platelet adhesion on surfaces and the structure versus the amount of adsorbed fibrinogen. Biomaterials 31, 832 (2010).CrossRefGoogle ScholarPubMed
Liu, X.L., Yuan, L., Li, D., Tang, Z.C., Wang, Y.W., Chen, G.J., Chen, H., and Brash, J.L.: Blood compatible materials: State of the art. J. Mater. Chem. B 2, 5718 (2014).CrossRefGoogle Scholar
Chen, Y.Q., Zhang, X., Zhao, S., Maitz, M.F., Zhang, W.T., Yang, S., Mao, J.L., Huang, N., and Wan, G.J.: In situ incorporation of heparin/bivalirudin into a phytic acid coating on biodegradable magnesium with improved anticorrosion and biocompatible properties. J. Mater. Chem. B 5, 4162 (2017).CrossRefGoogle Scholar
Wang, S., Sun, C., Guan, S., Li, W., Xu, J., Ge, D., Zhuang, M., Liu, T., and Ma, X.: Chitosan/gelatin porous scaffolds assembled with conductive poly(3,4-ethylenedioxythiophene) nanoparticles for neural tissue engineering. J. Mater. Chem. B 5, 4774 (2017).CrossRefGoogle Scholar