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Surface functionalization of cross-linked polystyrene microspheres via thiol–ene “click” reaction and assembly in honeycomb films for lectin recognition

Published online by Cambridge University Press:  21 January 2013

Xuan Yang ,
Liang-Wei Zhu ,
Ling-Shu Wan ,
Jing Zhang  and
Zhi-Kang Xu
Xuan Yang
Affiliation:
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering; Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China
Liang-Wei Zhu
Affiliation:
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering; Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China
Ling-Shu Wan*
Affiliation:
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering; Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China
Jing Zhang
Affiliation:
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering; Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China
Zhi-Kang Xu
Affiliation:
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering; Cyrus Tang Center for Sensor Materials and Applications, Zhejiang University, Hangzhou 310027, China
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

Patterned porous films prepared by the breath figure method have received considerable interests because of the potential applications. This paper reports a top–down method to fabricate functional patterned films. Cross-linked polystyrene microspheres were synthesized by a two-stage dispersion polymerization using divinylbenzene (DVB) and ethylene glycol dimethacrylate (EGDMA) as cross-linkers, which provide free vinyl groups on the microspheres surface. The amounts of residual vinyl groups were determined by potentiometric titration. Glucose was then bound to the microspheres via thiol–ene reaction, which was confirmed by x-ray photoelectron spectroscopy and water contact angle measurements. Results indicate that vinyl groups of EGDMA show relatively higher reactivity than that of DVB. Microspheres with glucose were assembled into the pores of honeycomb films prepared by the breath figure method, forming functional arrays for recognizing a lectin, Con A. This top–down method is useful in preparing patterned films with various functional moieties, which may act as a platform, such as, for investigating carbohydrate–lectin interactions and for sensing.

Keywords

two-stage dispersion polymerizationsurface chemistrythin film
Type
Articles
Information
Journal of Materials Research , Volume 28 , Issue 4 , 28 February 2013 , pp. 642 - 650
Copyright
Copyright © Materials Research Society 2013

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