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Protein functionalized nanostructured zirconia based electrochemical immunosensor for cardiac troponin I detection

Published online by Cambridge University Press:  24 April 2017

Suveen Kumar ,
Saurabh Kumar ,
Shine Augustine  and
Bansi D. Malhotra
Suveen Kumar
Affiliation:
Nano-bioelectronics Laboratory, Department of Biotechnology, Delhi Technological University, Delhi 110 042, India
Saurabh Kumar
Affiliation:
Nano-bioelectronics Laboratory, Department of Biotechnology, Delhi Technological University, Delhi 110 042, India
Shine Augustine
Affiliation:
Nano-bioelectronics Laboratory, Department of Biotechnology, Delhi Technological University, Delhi 110 042, India
Bansi D. Malhotra*
Affiliation:
Nano-bioelectronics Laboratory, Department of Biotechnology, Delhi Technological University, Delhi 110 042, India
*
a)Address all correspondence to this author. e-mail: [email protected]
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Abstract

We report results of the studies relating to the fabrication of nanostructured zirconia (nZrO2) based immunosensor for cardiac troponin I biomarker (acute myocardial infarction) detection. One step, low temperature hydrothermal process was used for the synthesis of nZrO2 (∼5 nm). This nZrO2 was functionalized with 3-aminopropyl triethoxy silane (APTES) and thereafter it was electrophoretically deposited on to indium tin oxide (ITO) coated glass electrode. EDC/NHS surface chemistry was used for covalent immobilization of monoclonal anti-troponin-I (anti-cTnI) antibodies onto APTES/nZrO2/ITO electrode. The structural, morphological and functional characterization of the synthesized nanoparticles and the fabricated immunoelectrode were conducted via X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and electrochemical techniques. The results of electrochemical response studies of BSA/anti-cTnI/APTES/nZrO2/ITO immunoelectrode reveal that, this platform can be used for efficient detection of cardiac troponin I (cTnI) biomarker with a wide linear detection range (0.1–100 ng/mL) and sensitivity [3.9 µA mL/(ng cm2)].

Keywords

cardiovascular diseasecardiac troponin IbiosensorzirconiaELISA
Type
Invited Articles
Information
Journal of Materials Research , Volume 32 , Issue 15: Focus Issue: Two-Dimensional Nanomaterials for Biosensors , 14 August 2017 , pp. 2966 - 2972
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Copyright
Copyright © Materials Research Society 2017 

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Footnotes

Contributing Editor: Venkatesan Renugopalakrishnan

References

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