Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-20T09:39:52.488Z Has data issue: false hasContentIssue false
  • English
  • Français

Tacrolimus enhances the recovery of normal laryngeal muscle fibre distribution after reinnervation

Published online by Cambridge University Press:  04 September 2012

P Gorphe ,
N Guerout ,
M Birchall ,
G Terenghi  and
J-P Marie
P Gorphe*
Affiliation:
Otorhinolaryngology and Head and Neck Surgery Department, Rouen University Hospital, France Experimental Surgery Laboratory, Faculty of Medicine, Rouen University, France Otorhinolaryngology and Head and Neck Surgery Department, Hôpital Avicenne, Bobigny, France
N Guerout
Affiliation:
Experimental Surgery Laboratory, Faculty of Medicine, Rouen University, France
M Birchall
Affiliation:
UCL Ear Hospital, University College London, UK
G Terenghi
Affiliation:
Blond McIndoe Laboratories, Tissue Injury and Repair Group, School of Medicine, University of Manchester, UK
J-P Marie
Affiliation:
Otorhinolaryngology and Head and Neck Surgery Department, Rouen University Hospital, France Experimental Surgery Laboratory, Faculty of Medicine, Rouen University, France
*
Address for correspondence: Dr Philippe Gorphe, Otorhinolaryngology and Head and Neck Surgery Department, Rouen University Hospital, 76031 Rouen, France E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Objectives:

To assess the recovery of various muscle fibre types in the posterior cricoarytenoid muscle after laryngeal reinnervation in the rat, and to determine the influence of tacrolimus on this process.

Methods:

Four groups of rats underwent resection and anastomosis of the left vagus nerve, and were administered either tacrolimus at a low dose or an immunosuppressive dose, or cyclosporin A at a low dose or an immunosuppressive dose. A fifth group received surgery alone, and a sixth group received neither surgery nor drug treatment (healthy group). Muscles were removed for immunohistochemical analysis 45 days after surgery.

Results:

There was no difference in the proportion of types 1, 2a and 2b muscle fibres, comparing the immunosuppressive tacrolimus group and the healthy group, whereas there were fewer type 1 fibres in the group receiving surgery alone, compared with the healthy group (7 vs 12.1 per cent, respectively; p = 0.0303).

Conclusion:

Tacrolimus enhanced the recovery of normal laryngeal muscle fibres after reinnervation in the rat, indicating a possible role in laryngeal transplantation.

Keywords

TacrolimusLaryngeal ReinnervationCalcineurin InhibitorsMuscle Fibers
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 126 , Issue 11 , November 2012 , pp. 1155 - 1158
Copyright
Copyright © JLO (1984) Limited 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Presented in part at the Congress of the European Laryngological Society, 1st September 2006, Nottingham, UK

References

1 Birchall, M, Macchiarini, P. Airway transplantation: a debate worth having? Transplantation 2008;85:1075–80CrossRefGoogle Scholar
2 Birchall, MA, Lorenz, RR, Berke, GS, Genden, EM, Haughey, BH, Siemionow, M et al. Laryngeal transplantation in 2005: a review. Am J Transplant 2006;6:20–6CrossRefGoogle ScholarPubMed
3 Marie, JP, Dehesdin, D, Ducastelle, T, Senant, J. Selective reinnervation of the abductor and adductor muscles of the canine larynx after recurrent nerve paralysis. Ann Otol Rhinol Laryngol 1989;98:530–6CrossRefGoogle ScholarPubMed
4 Gold, BG, Villafranca, JE. Neuroimmunophilin ligands: the development of novel neuroregenerative/neuroprotective compounds. Curr Top Med Chem 2003;3:1368–75CrossRefGoogle ScholarPubMed
5 Chen, B, Song, Y, Liu, Z. Promotion of nerve regeneration in peripheral nerve by short-course FK506 after end-to-side neurorrhaphy. J Surg Res 2009;152:303–10CrossRefGoogle ScholarPubMed
6 Yildirim, FB, Sarikcioglu, L, Ozsoy, U, Demir, N, Demirtop, A, Ucar, Y. Effect of FK506 administration after obturator nerve injury: a functional and ultrastructural study. Acta Neurobiol Exp (Wars) 2008;68:477–83CrossRefGoogle ScholarPubMed
7 Hontanilla, B, Auba, C, Arcocha, J, Gorria, O. Nerve regeneration through nerve autografts and cold preserved allografts using tacrolimus (FK506) in a facial paralysis model: a topographical and neurophysiological study in monkeys. Neurosurgery 2006;58:768–79CrossRefGoogle Scholar
8 Sosa, I, Reyes, O, Kuffler, DP. Immunosuppressants: neuroprotection and promoting neurological recovery following peripheral nerve and spinal cord lesions. Exp Neurol 2005;195:7–15 CrossRefGoogle ScholarPubMed
9 Kuffler, DP. Enhancement of nerve regeneration and recovery by immunosuppressive agents. Int Rev Neurobiol 2009;87:347–62CrossRefGoogle ScholarPubMed
10 Dunn, SE, Simard, AR, Prud'homme, RA, Michel, RN. Calcineurin and skeletal muscle growth. Nat Cell Biol 2002;4:E46CrossRefGoogle ScholarPubMed
11 DelGaudio, JM, Sciote, JJ. Changes in myosin expression in denervated laryngeal muscle. Ann Otol Rhinol Laryngol 1997;106:1076–81CrossRefGoogle ScholarPubMed
12 Kingham, PJ, Birchall, MA, Burt, R, Jones, A, Terenghi, G. Reinnervation of laryngeal muscles: a study of changes in myosin heavy chain expression. Muscle Nerve 2005;32:761–6CrossRefGoogle ScholarPubMed
13 Wu, YZ, Baker, MJ, Marie, JP, Crumley, R, Caiozzo, VJ. The plasticity of denervated and reinnervated laryngeal muscle: focus on single-fibre myosin heavy-chain isoform expression. Arch Otolaryngol Head Neck Surg 2004;130:1070–82CrossRefGoogle Scholar
14 Rhee, HS, Lucas, CA, Hoh, JF. Fibre types in rat laryngeal muscles and their transformations after denervation and reinnervation. J Histochem Cytochem 2004;52:581–90CrossRefGoogle ScholarPubMed
15 Aydin, MA, Urbanchek, MG, Kuzon, WM. Improved early muscle recovery using FK506 in a rat nerve-repair model. J Reconstr Microsurg 2004;20:183–92Google Scholar