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Significance of fascin expression in laryngeal squamous cell carcinoma

Published online by Cambridge University Press:  19 November 2009

A Durmaz ,
B Kurt ,
O Ongoru ,
S Karahatay ,
M Gerek  and
S Yalcın
A Durmaz*
Affiliation:
Department of Otorhinolaryngology Head and Neck Surgery, Gulhane Military Medical Academy, Ankara, Turkey
B Kurt
Affiliation:
Department of Pathology, Gulhane Military Medical Academy, Ankara, Turkey
O Ongoru
Affiliation:
Department of Pathology, Gulhane Military Medical Academy, Ankara, Turkey
S Karahatay
Affiliation:
Department of Otorhinolaryngology Head and Neck Surgery, Gulhane Military Medical Academy, Ankara, Turkey
M Gerek
Affiliation:
Department of Otorhinolaryngology Head and Neck Surgery, Gulhane Military Medical Academy, Ankara, Turkey
S Yalcın
Affiliation:
Department of Pathology, Gulhane Military Medical Academy, Ankara, Turkey
*
Address for correspondence: Dr Abdullah Durmaz, Assistant Professor, Department of ORL and HNS, GATA Etlik 06018, Ankara, Turkey. Fax: +90 312 304 5700 E-mail: [email protected]
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Abstract

Objective:

Fascin is an actin-binding protein which is expressed in the basal areas of healthy squamous epithelium. Although overexpression of fascin has been shown in many tumours, the relationship between fascin and laryngeal squamous cell carcinoma has not previously been investigated, to the best of our knowledge. This study aimed to investigate the relationship between fascin expression and tumour behaviour in 30 cases of laryngeal squamous cell carcinoma.

Materials and methods:

For all lesions, a section of paraffin-embedded tissue was immunohistochemically stained for fascin. The percentage of positive, stained cells was scored from one to five (one = 0–5 per cent, two = 6–25 per cent, three = 26–50 per cent, four = 51–75 per cent and five = 76–100 per cent), and the staining intensity from one to three (one = mild, two = moderate and three = strong). A total immunohistochemical fascin expression score was obtained by multiplying the staining percentage and intensity. The relationship between the total fascin score and each case's age, sex, tumour localisation, tumour–node–metastasis stage and differentiation was evaluated statistically.

Results:

Various amounts of fascin expression were observed in all cases. There was a statistically significant relationship between high levels of fascin expression (i.e. a total fascin score of 10 or more) and the cases' tumour stage (p = 0.022), node stage (p = 0.024) and clinical stage (p = 0.014). In addition, worsening tumour differentiation was associated with an increasing fascin score, but this finding was statistically insignificant.

Conclusion:

These results suggest that laryngeal squamous cell carcinomas with high levels of fascin expression may be more aggressive than those with low expression levels. Further studies with larger series are needed to support these results and to clarify rationales.

Keywords

Larynx NeoplasmsSquamous Cell CarcinomaFascin
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 124 , Issue 2 , February 2010 , pp. 194 - 198
Copyright
Copyright © JLO (1984) Limited 2009

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References

1Chu, EA, Kim, YJ. Laryngeal cancer: diagnosis and preoperative work-up. Otolaryngol Clin North Am 2008;41:637–95CrossRefGoogle ScholarPubMed
2Yilmaz, T, Gedikoglu, G, Gursel, B. The relationship between tumor thickness and clinical and histopathologic parameters in cancer of the larynx. Otolaryngol Head Neck Surg 2003;129:192–8CrossRefGoogle ScholarPubMed
3Loyo, M, Pai, S. The molecular genetics of laryngeal cancer. Otolaryngol Clin North Am 2008;41:657–72CrossRefGoogle ScholarPubMed
4Adams, JC. Roles of fascin in cell adhesion and motility. Curr Opin Cell Biol 2004;16:590–6CrossRefGoogle ScholarPubMed
5Kureishy, N, Sapountzi, V, Prag, S, Anilkumar, N, Adams, JC. Fascins and their roles in cell structure and function. Bioessays 2002;24:350–61CrossRefGoogle ScholarPubMed
6Chen, G, Zhang, FR, Ren, J, Tao, LH, Shen, ZY, Lv, Z et al. Expression of fascin in thyroid neoplasms: a novel diagnostic marker. J Cancer Res Clin Oncol 2008;134:947–51CrossRefGoogle ScholarPubMed
7Hwang, JH, Smith, CA, Salhia, B, Rutka, JT. The role of fascin in the migration and invasiveness of malignant glioma cells. Neoplasia 2008;10:149–59CrossRefGoogle ScholarPubMed
8Sarrió, D, Rodriguez-Pinilla, SM, Hardisson, D, Cano, A, Moreno-Bueno, G, Palacios, J. Epithelial-mesenchymal transition in breast cancer relates to the basal-like phenotype. Cancer Res 2008;68:989–97CrossRefGoogle Scholar
9Lee, TK, Poon, RT, Man, K, Guan, XY, Ma, S, Liu, XB et al. Fascin over-expression is associated with aggressiveness of oral squamous cell carcinoma. Cancer Lett 2007;254:308–15CrossRefGoogle ScholarPubMed
10Gunal, A, Onguru, O, Safali, M, Beyzadeoglu, M. Fascin expression in glial tumors and its prognostic significance in glioblastomas. Neuropathology 2008;28:382–6CrossRefGoogle ScholarPubMed
11Grothey, A, Hashizume, R, Ji, H, Tubb, BE, Patrick, CW Jr, Yu, D et al. C-erbB-2/HER-2 upregulates fascin, an actin-bundling protein associated with cell motility, in human breast cancer cell lines. Oncogene 2000;19:4864–75CrossRefGoogle ScholarPubMed
12Zhang, H, Xu, L, Xiao, D, Xie, J, Zeng, H, Cai, W et al. Fascin is a potential biomarker for early-stage oesophageal squamous cell carcinoma. J Clin Pathol 2006;59:958–64CrossRefGoogle ScholarPubMed
13Hashimoto, Y, Shimada, Y, Kawamura, J, Yamasaki, S, Imamura, M. The prognostic relevance of fascin expression in human gastric carcinoma. Oncology 2004;67:262–70CrossRefGoogle ScholarPubMed
14Pelosi, G, Pastorino, U, Pasini, F, Maissoneuve, P, Fraggetta, F, Iannucci, A et al. Independent prognostic value of fascin immunoreactivity in stage I nonsmall cell lung cancer. Br J Cancer 2003;88:537–47CrossRefGoogle ScholarPubMed
15Jawhari, AU, Buda, A, Jenkins, M, Shehzad, K, Sarraf, C, Noda, M et al. Fascin, an actin-bundling protein, modulates colonic epithelial cell invasiveness and differentiation in vitro. Am J Pathol 2003;162:69–80CrossRefGoogle ScholarPubMed
16Swierczynski, SL, Maitra, A, Abraham, SC, Iacobuzio-Donahue, CA, Ashfaq, R, Cameron, JL. Analysis of novel tumor markers in pancreatic and biliary carcinomas using tissue microarrays. Hum Pathol 2004;35:357–66CrossRefGoogle ScholarPubMed
17Hu, W, McCrea, PD, Deavers, M, Kavanagh, JJ, Kudelka, AP, Verschraegen, CF. Increased expression of fascin, motility associated protein, in cell cultures derived from ovarian cancer and in borderline and carcinomatous ovarian tumors. Clin Exp Metastasis 2000;18:83–8CrossRefGoogle ScholarPubMed
18Tong, GX, Yee, H, Chiriboga, L, Hernandez, O, Waisman, J. Fascin-1 expression in papillary and invasive urothelial carcinomas of the urinary bladder. Hum Pathol 2005;36:741–6CrossRefGoogle ScholarPubMed
19Demiral, AN, Sarioglu, S, Birlik, B, Sen, M, Kinay, M. Prognostic significance of EGF receptor expression in early glottic cancer. Auris Nasus Larynx 2004;31:417–24CrossRefGoogle ScholarPubMed
20Kyzas, PA, Stefanou, D, Batistatou, A, Agnantis, NJ, Nakanishi, Y, Hirohashi, S et al. Dysadherin expression in head and neck squamous cell carcinoma: association with lymphangiogenesis and prognostic significance. Am J Surg Pathol 2006;30:185–93CrossRefGoogle ScholarPubMed
21Kyzas, PA, Stefanou, D, Agnantis, NJ. COX-2 expression correlates with VEGF-C and lymph node metastases in patients with head and neck squamous cell carcinoma. Mod Pathol 2005;18:153–60CrossRefGoogle ScholarPubMed
22Chen, SF, Yang, SF, Li, JW, Nieh, PC, Lin, SY, Fu, E et al. Expression of fascin in oral and oropharyngeal squamous cell carcinomas has prognostic significance – a tissue microarray study of 129 cases. Histopathology 2007;51:173–83CrossRefGoogle ScholarPubMed
23Yamashiro, S, Yamakita, Y, Ono, S, Matsumura, F. Fascin, an actin-bundling protein, induces membrane protrusions and increases cell motility of epithelial cells. Mol Biol Cell 1998;9:993–1006CrossRefGoogle ScholarPubMed