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Protective effects of brain-derived neurotrophic factor on the noise-damaged cochlear spiral ganglion

Published online by Cambridge University Press:  16 November 2010

S-Q Zhai ,
W Guo ,
Y-Y Hu ,
N Yu ,
Q Chen ,
J-Z Wang ,
M Fan  and
W-Y Yang
S-Q Zhai*
Affiliation:
Institute of Otolaryngology-Head and Neck Surgery, Chinese People's Liberation Army General Hospital, Beijing, China
W Guo
Affiliation:
Institute of Otolaryngology-Head and Neck Surgery, Chinese People's Liberation Army General Hospital, Beijing, China
Y-Y Hu
Affiliation:
Institute of Otolaryngology-Head and Neck Surgery, Chinese People's Liberation Army General Hospital, Beijing, China
N Yu
Affiliation:
Institute of Otolaryngology-Head and Neck Surgery, Chinese People's Liberation Army General Hospital, Beijing, China
Q Chen
Affiliation:
Institute of Basic Medical Science, Academy of Military Medical Sciences of People's Liberation Army, Beijing, China
J-Z Wang
Affiliation:
Institute of Basic Medical Science, Academy of Military Medical Sciences of People's Liberation Army, Beijing, China
M Fan
Affiliation:
Institute of Basic Medical Science, Academy of Military Medical Sciences of People's Liberation Army, Beijing, China
W-Y Yang
Affiliation:
Institute of Otolaryngology-Head and Neck Surgery, Chinese People's Liberation Army General Hospital, Beijing, China
*
Address for correspondence: Dr Suo-Qiang Zhai, 28 Fuxing Road, Haidian District, Beijing 100853, China Fax: 86 024 23380681 E-mail: [email protected]
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Abstract

Objective:

To explore the protective effects of brain-derived neurotrophic factor on the noise-damaged cochlear spiral ganglion.

Methods:

Recombinant adenovirus brain-derived neurotrophic factor vector, recombinant adenovirus LacZ and artificial perilymph were prepared. Guinea pigs with audiometric auditory brainstem response thresholds of more than 75 dB SPL, measured seven days after four hours of noise exposure at 135 dB SPL, were divided into three groups. Adenovirus brain-derived neurotrophic factor vector, adenovirus LacZ and perilymph were infused into the cochleae of the three groups, variously. Eight weeks later, the cochleae were stained immunohistochemically and the spiral ganglion cells counted.

Results:

The auditory brainstem response threshold recorded before and seven days after noise exposure did not differ significantly between the three groups. However, eight weeks after cochlear perfusion, the group receiving brain-derived neurotrophic factor had a significantly decreased auditory brainstem response threshold and increased spiral ganglion cell count, compared with the adenovirus LacZ and perilymph groups.

Conclusion:

When administered via cochlear infusion following noise damage, brain-derived neurotrophic factor appears to improve the auditory threshold, and to have a protective effect on the spiral ganglion cells.

Keywords

CochleaNoise-Induced Hearing LossBrain-Derived Neurotrophic FactorGuinea Pig
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 125 , Issue 5 , May 2011 , pp. 449 - 454
Copyright
Copyright © JLO (1984) Limited 2010

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