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The protective effect of Ginkgo biloba extract against experimental cisplatin ototoxicity: animal research using distortion product otoacoustic emissions

Published online by Cambridge University Press:  14 September 2012

B Cakil ,
F Suren Basar ,
S Atmaca ,
S Kurnaz Cengel ,
A Tekat  and
Y Tanyeri
B Cakil
Affiliation:
Department of ENT, Ondokuz Mayis University School of Medicine, Samsun, Turkey
F Suren Basar*
Affiliation:
Department of Audiology, Ondokuz Mayis University School of Medicine, Samsun, Turkey
S Atmaca
Affiliation:
Department of ENT, Ondokuz Mayis University School of Medicine, Samsun, Turkey
S Kurnaz Cengel
Affiliation:
Department of ENT, Ondokuz Mayis University School of Medicine, Samsun, Turkey
A Tekat
Affiliation:
Department of ENT, Ondokuz Mayis University School of Medicine, Samsun, Turkey
Y Tanyeri
Affiliation:
Department of ENT, Ondokuz Mayis University School of Medicine, Samsun, Turkey
*
Address for correspondence: Dr F Suren Basar, Ondokuz Mayis Üniversitesi Hastanesi, KBB Anabilim Dali, Odyoloji Ünitesi, Kurupelit 55139, Samsun, Turkey Fax: +90 362 457 6041 E-mail: [email protected]
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Abstract

Background:

Cisplatin, an effective therapeutic agent for various human cancers, has dose-limiting side effects of ototoxicity and nephrotoxicity. Cisplatin ototoxicity is thought to result from increased amounts of toxic free radicals or cell membrane changes leading to increased intracellular calcium content. Ginkgo biloba extract prevents lipid peroxidation, decreases intracellular free oxygen radical levels, regulates the cell membrane calcium transport mechanism and prevents cell death. This study aimed to investigate the protective effect of Ginkgo biloba extract against cisplatin-induced ototoxicity in rats.

Methods:

Twenty Wistar albino rats with normal hearing (confirmed by distortion product otoacoustic emission testing prior to cisplatin application) were randomly allocated to two groups. Both groups received a single intraperitoneal dose of cisplatin (12 mg/kg). Group two also received daily intraperitoneal doses of Ginkgo biloba extract (100 mg/kg) for 10 days. Distortion product otoacoustic emission measurements were repeated on days 10 and 17 and signal-to-noise ratios were compared.

Results:

Compared with group one, group two had significantly better distortion product otoacoustic emission results at 3, 4, 6 and 8 kHz on days 10 and 17.

Conclusion:

These findings suggest that Ginkgo biloba extract protects the inner ear against cisplatin-induced ototoxicity.

Keywords

Ear, InnerCisplatinGinkgo BilobaOtotoxicityPrevention And Control
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 126 , Issue 11 , November 2012 , pp. 1097 - 1101
Copyright
Copyright © JLO (1984) Limited 2012

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Footnotes

Presented at the 30th Turkish National Otorhinolaryngology and Head and Neck Surgery Congress, 8–12 October 2008, Antalya, Turkey

References

1 Roland, PS, Rutka, JA. Ototoxicity. Hamilton: BC Decker, 2004;93–4Google ScholarPubMed
2 Aslan, E, Orzan, E, Santorelli, R. Global problem of drug-induced hearing loss. Ann N Y Acad Sci 1999;884:1–14 CrossRefGoogle Scholar
3 Kalcioglu, MT, Kizilay, A, Gulec, M, Karatas, E, Iraz, M, Akyol, O et al. The protective effect of erdosteine against ototoxicity induced by cisplatin in rats. Eur Arch Otorhinolaryngol 2005;262:856–63CrossRefGoogle ScholarPubMed
4 Choe, WT, Chinosornvatana, N, Chang, KW. Prevention of cisplatin ototoxicity using transtympanic N-acetylcysteine and lactate. Otol Neurotol 2004;25:910–15CrossRefGoogle ScholarPubMed
5 Hyppolito, MA, de Oliveire, AA, Lessa, RM, Rossato, M. Amifostine otoprotection to cisplatin ototoxicity: a guinea pig study using otoacoustic emission distortion products (DPOEA) and scanning electron microscopy. Braz J Otorhinolaryngol 2005;71:268–73CrossRefGoogle ScholarPubMed
6 Kalkanis, JG, Whitworth, C, Rybak, LP. Vitamin E reduces cisplatin ototoxicity. Laryngoscope 2004;114:538–42CrossRefGoogle ScholarPubMed
7 Biro, K, Noszek, L, Prekopp, P, Nagyiványi, K, Géczi, L, Gaudi, I et al. Characteristics and risk factors of cisplatin-induced ototoxicity in testicular cancer patients detected by distortion product otoacoustic emission. Oncology 2006;70:177–84CrossRefGoogle ScholarPubMed
8 Strauss, M, Towfighi, J, Lord, S, Lipton, A, Harvey, HA, Brown, B. Cis-platinum ototoxicity: clinical experience and temporal bone histopathology. Laryngoscope 1983;93:1554–9CrossRefGoogle ScholarPubMed
9 Liang, F, Schulte, BA, Qu, C, Hu, W, Shen, Z. Inhibition of the calcium- and voltage-dependent big conductance potassium channel ameliorates cisplatin-induced apoptosis in spiral ligament fibrocytes of the cochlea. Neuroscience 2005;135:261–71CrossRefGoogle ScholarPubMed
10 Rybak, LP, Whitworth, CA, Mukherjea, D, Ramkumar, V. Mechanisms of cisplatin-induced ototoxicity and prevention. Hear Res 2007;226:157–67CrossRefGoogle ScholarPubMed
11 Komune, S, Asakuma, S, Snow, JB Jr. Pathophysiology of the ototoxicity of cis-diamminedichloroplatinum. Otolaryngol Head Neck Surg 1981;89:275–82CrossRefGoogle ScholarPubMed
12 Devarajan, P, Savoca, M, Casteneda, MP, Park, MS, Esteban-Cruciani, N, Kalinec, G et al. Cisplatin-induced apoptosis in auditory cells: role of death receptor and mitochondrial pathways. Hear Res 2002;174:45–54 CrossRefGoogle ScholarPubMed
13 Sha, SH, Taylor, R, Forge, A, Schacht, J. Differential vulnerability of basal and apical hair cells based on intrinsic susceptibility to free radicals. Hear Res 2001;155:1–8 CrossRefGoogle ScholarPubMed
14 Kemp, DT. Stimulated acoustic emissions from within the human auditory system. J Acoust Soc Am 1978;64:1386–91CrossRefGoogle ScholarPubMed
15 Prieve, B, Fitzgerald, T. Otoacoustic, emissions. In: Katz, J, Medwetsky, L, Burkard, R, Hood, L, eds. Handbook of Clinical Audiology. Philadelphia: Lippincott Williams & Wilkins, 2009;497–528 Google Scholar
16 Lonsbury-Martin, BL, Martin, GK, Telischi, FF. Otoacoustic emissions in clinical practice. In: Musiek, FE, Rintelmann, WF, eds. Contemporary Perspectives in Hearing Assessment. Boston: Allyn & Bacon, 1999;167–196 Google Scholar
17 Liu, KX, Wu, WK, He, W, Liu, CL. Gingko biloba extract (EGb 761) attenuates lung injury induced by intestinal ischemia/reperfusion in rats: roles of oxidative stress and nitric oxide. World J Gastroenterol 2007;13:299–305 CrossRefGoogle ScholarPubMed
18 Tozan, A, Sehirli, O, Omurtag, GZ, Cetinel, S, Gedik, N, Sener, G. Gingko biloba extract reduces naphthalene-induced oxidative damage in mice. Phytother Res 2007;21:72–7CrossRefGoogle ScholarPubMed
19 Gulec, M, Iraz, M, Yilmaz, HR, Ozyurt, H, Temel, I. The effects of gingko biloba extract on tissue adenosine deaminase, xanthine oxidase, myeloperoxidase, malondialdehyde and nitric oxide in cisplatin-induced nephrotoxicity. Toxicol Ind Health 2006;22:125–30CrossRefGoogle ScholarPubMed
20 Rybak, LP. Ototoxin-induced hearing loss. In: Clark, WW, Ohlemiller, KK, eds. Anatomy and Physiology of Hearing for Audiologists. New York: Thomson Delmar Learning, 2008;344–361 Google Scholar
21 Rybak, LP, Rankumar, V. Ototoxicity. Kidney Int 2007;72:931–5CrossRefGoogle ScholarPubMed
22 Pollera, CF, Marolla, P, Nardi, M, Ameglio, F, Cozzo, L, Bevere, F. Very high-dose cisplatin-induced ototoxicity: a preliminary report on early and long-term effects. Cancer Chemother Pharmacol 1988;21:61–4CrossRefGoogle ScholarPubMed
23 Lopez-Gonzalez, MA, Guerrero, JM, Rojas, F, Delgado, F. Ototoxicity caused by cisplatin is ameliorated by melatonin and other antioxidants. J Pineal Res 2000;28:73–80 CrossRefGoogle ScholarPubMed
24 Cardinaal, RM, de Groot, JC, Huizing, EH, Veldman, JE, Smoorenburg, GF. Dose-dependent effect of 8-day cisplatin administration upon the morphology of the albino guinea pig cochlea. Hear Res 2000;144:135–46CrossRefGoogle ScholarPubMed
25 Huang, X, Whitworth, CA, Rybak, LP. Ginkgo biloba extract (EGb 761) protects against cisplatin-induced ototoxicity in rats. Otol Neurotol 2007;28:828–33CrossRefGoogle ScholarPubMed
26 Meech, RP, Campbell, KCM, Hughes, LP, Rybak, LP. A semiquantitative analysis of the effects of cisplatin on the rat stria vascularis. Hear Res 1998;124:44–59 CrossRefGoogle ScholarPubMed