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Nuclear DNA content using computerized image cytometry of squamous cell carcinomas of the head and neck

Published online by Cambridge University Press:  29 June 2007

S. K. Sarker ,
K. Ghufoor ,
K. S. Patel ,
N. S. Tolley  and
D. V. Coleman
S. K. Sarker*
Affiliation:
Cytopathology, Cytogenetics, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK. Academic Surgical, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK Head and Neck, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK.
K. Ghufoor
Affiliation:
Head and Neck, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK.
K. S. Patel
Affiliation:
Head and Neck, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK.
N. S. Tolley
Affiliation:
Head and Neck, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK.
D. V. Coleman
Affiliation:
Cytopathology, Cytogenetics, Units, St Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, University of London, London, UK.
*
Address for correspondence: S. K. Sarker, Cytopathology and Cytogenetics Unit, Clarence Wing, St Mary's Hospital Medical School, Praed Street, London W2 1NY. Fax: 0171-725-1262
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Abstract

Ploidy status using flow cytometry of head and neck cancers may be of prognostic value. We describe the use of image cytometry in ploidy measurement of squamous cell carcinomas of the head and neck (SCCHN). This technique allows only tumour cells to be measured, thereby rejecting debris, artefact and benign cells. Tissue sections were cut from tumours and then Feulgen stained. A total of 60 patients were included in this study, 23 females and 37 males. The data reveals a relationship between ploidy status and the histological differentiation. However, the ploidy status and histological differentiation do not appear to correlate to the clinical stage of the disease. This method of measuring ploidy may be more accurate than flow cytometry and may have a prognostic role in head and neck cancer patients. A study comparing both methods may demonstrate this and we aim to evaluate this in the future.

Keywords

DNAImage cytometryHead and neck neoplasmsCarcinomasquamous cell
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 111 , Issue 1 , January 1997 , pp. 43 - 47
Copyright
Copyright © JLO (1984) Limited 1997

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References

Bacus, J. W., Grace, L. J. (1987) Optical microscope system for standardised cell measurements and analysis. Applied Optics 26–3280.Google Scholar
Barlogie, B., Raber, M. N., Schuman, J. (1983) Flow cytometry in clinical cancer research. Cancer Research 43–3982.Google ScholarPubMed
Boecking, A., Auffermann, W., Vogel, H., Scholoendorff, G., Goebbels, R. (1985) Diagnosis and grading of malignancy in sqaumous epithelial lesions of the larynx with DNA cytophotometry. Cancer 56–1600.Google Scholar
Buchner, T., Hiddermann, W., Wormann, B. (1985) Differential patterns of DNA aneuploidy in human malignancies. Pathology Research 179–310.Google ScholarPubMed
Chen, R. B. (1990) Flow cytometric analysis of benign and malignant tumours of the oral and maxillofacial region. Journal of Oral and Maxillofacial Surgery 47–596.Google Scholar
Cooke, L. D., Cooke, T. G., Bootz, F., Forster, G., Helliwell, T. R., Spiller, D., Stell, P. M. (1990) Ploidy as a prognostic indicator in end stage squamous cell carcinoma of the head and neck region treated with cisplatinum. British Journal of Cancer 61–759.Google ScholarPubMed
Cornelisse, C. J., van de Velde, C. J. H., Caspers, R. J. C., Moolenar, A. J., Hermans, J. (1987) DNA ploidy and survival in breast cancer patients. Cytometry 8: 225.CrossRefGoogle ScholarPubMed
Deitch, A. (1966) Cytophotometry of nucleic acids. In (Weid, G.L., ed.) Introduction to Quantitative Cytochemistry. Academic Press, London, pp327–354.Google Scholar
Fu, Y. S., Hall, T. L. (1985) DNA ploidy measurements in tissue sections. Analytical and Quantitative Cytology and Histology 7: 90.Google ScholarPubMed
Goldsmith, M. M., Cresson, D. S., Postma, D. S., Askin, F. B., Pillsbury, H. C. (1986) Significance of ploidy in laryngeal cancer. American Journal of Surgery 152–396.Google ScholarPubMed
Goldsmith, M. M., Cresson, D. H., Arnold, L. A., Postma, D. S., Askin, F. B., Pillsbury, H. C. (1987) DNA flow cytometry as a prognostic indicator in head and neck cancer. Otolaryngology – Head and Neck Surgery 96: 307–318.CrossRefGoogle ScholarPubMed
Guo, Y. C., Desanto, L., Osetinsky, G. V. (1989) Prognostic implications of nuclear DNA content in head and neck cancer. Otolaryngology-Head and Neck Surgery 100–95.Google ScholarPubMed
Holm, L. (1982) Cellular DNA amounts of squamous cell carcinomas of the head and neck region in relation to prognosis. Laryngoscope 92: 1064–1069.CrossRefGoogle ScholarPubMed
Joensuu, H., Klemi, P., Eerola, E., Tuominen, J. (1986) Influence of cellular DNA content on survival in differentiated thyroid carcinoma. Cancer 58: 2462.3.0.CO;2-E>CrossRefGoogle Scholar
Kearsley, J. H., Bryson, G., Battistutta, D., Collins, R. J. (1991) Prognostic importance of cellular DNA content in head and neck squamous cell cancers. A comparison of retrospective and prospective series. International Journal of Cancer 47–31.Google ScholarPubMed
Kokal, W. A., Gardine, R. L., Sheibani, K., Zak, I., Beatty, D., Riihimaki, D. U., Wagman, L. D., Terz, J. J. (1988) Tumour DNA content as a prognostic indicator in squamous cell carcinoma of the head and neck region. American Journal of Surgery 156–276.Google ScholarPubMed
Lampe, H. B., Flint, A., Wolf, G. T., McClatchey, K. D. (1987) Flow cytometry: DNA analysis of squamous cell carcinomas of the upper aerodigestive tract. Journal of Otolaryngology 16–371.Google ScholarPubMed
Mayall, B. H. (1988) Current capabilities and clinical applications of image cytometry. Cytometry 3 (Suppl.): 78–84.CrossRefGoogle ScholarPubMed
Mellin, W. (1990) Cytophotometry in tumour pathology. A critical review of methods and application and some results of DNA analysis. Pathology Research Practice 186–37.Google Scholar
Sandwritter, W., Carl, M., Ritter, W. (1966) Cytomorphometric measurements of the DNA content of human malignant tumors by means of the Feulgen reaction. Acta Cytologica (Baltimore) 10–26.Google Scholar
Sickle-Santanello, B. J., Farrar, W. B., Dobson, J. B., O'Toole, R. V., Keyhani-Rofagha, S. (1986) Flow cytometric analysis of DNA content as a prognostic indicator in squamous cell carcinoma of the tongue. American Journal of Surgery 152: 393–395.CrossRefGoogle ScholarPubMed
Stell, P. M. (1991) Ploidy in head and neck cancer: a review and meta-analysis. Clinical Otolaryngology 16: 16–510.CrossRefGoogle ScholarPubMed
Tytor, M., Franzen, G., Olofsson, J. (1989) DNA ploidy in oral cavity carcinomas with special reference to prognosis. Head and Neck 11: 11–257.CrossRefGoogle ScholarPubMed
Volm, M. (1990) Prognostic implications of DNA aneuploidy and proliferative activity in solid human tumours. Tumour Diagnosio and Therapy Ther 10: 10–229.Google Scholar
Westerbeek, H. A., Mooi, W. J., Hilgers, F. J. M., Baris, G., Begg, A. C., Begg, A. C., Balm, A. J. M. (1993) Ploidy status and the response of T1 glottic carcinoma to radiotherapy. Clinical Otolaryngology 18: 18–98.CrossRefGoogle ScholarPubMed
Zimmerman, P. V., Hawson, G. A. T., Bint, M. H., Parsons, P. G. (1987) Ploidy as a prognostic determinant in surgically treated lung cancer. Lancet ii: 530.CrossRefGoogle Scholar