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Mycoplasma pneumoniae and Chlamydophila pneumoniae: a comparative study in patients with nasal polyposis and healthy controls

Published online by Cambridge University Press:  30 July 2015

D G Ioannidis ,
V A Lachanas ,
Z Florou ,
J G Bizakis ,
E Petinaki  and
C E Skoulakis
D G Ioannidis
Affiliation:
Department of Microbiology, University of Thessaly, Larissa, Greece
V A Lachanas
Affiliation:
Department of Otorhinolaryngology, University Hospital of Larissa, Greece
Z Florou
Affiliation:
Department of Microbiology, University of Thessaly, Larissa, Greece
J G Bizakis
Affiliation:
Department of Otorhinolaryngology, University Hospital of Larissa, Greece
E Petinaki*
Affiliation:
Department of Microbiology, University of Thessaly, Larissa, Greece
C E Skoulakis
Affiliation:
Department of Otorhinolaryngology, University Hospital of Larissa, Greece
*
Address for correspondence: Dr Efthymia Petinaki, Department of Microbiology, University of Thessaly Viopolis, PC 41110 Larissa, Greece Fax: +30 241 3 501570 E-mail: [email protected]
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Abstract

Introduction:

The role played by Mycoplasma pneumoniae and Chlamydophila pneumoniae in the pathogenesis of chronic rhinosinusitis with nasal polyps has been the object of ongoing debate. We used real-time polymerase chain reaction to investigate the prevalence of both microorganisms in the nasal tissue samples of patients and controls.

Methods:

We extracted DNA from nasal polyp samples obtained during functional endoscopic sinus surgery and the inferior turbinate samples of controls undergoing septoplasty. We used the highly sensitive real-time polymerase chain reaction to detect the presence of M pneumoniae and C pneumoniae DNA.

Results:

Patients with chronic rhinosinusitis with nasal polyps consisted of 62 individuals (39 men; mean age 51 years); the control group consisted of 24 individuals (13 men; mean age 45 years). All samples from both groups were negative for M pneumoniae and C pneumoniae DNA.

Conclusion:

We have demonstrated that the likelihood of M pneumoniae and C pneumoniae acting as an ongoing inflammatory stimulus in chronic rhinosinusitis with nasal polyps is slim.

Keywords

Nasal PolypsMycoplasma pneumoniaeChlamydophila pneumoniaeReal-Time Polymerase Chain Reaction
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 129 , Issue 9 , September 2015 , pp. 865 - 869
Copyright
Copyright © JLO (1984) Limited 2015 

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