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A murine model of subglottic granulation

Published online by Cambridge University Press:  15 February 2016

A Ghosh ,
K P Leahy ,
S Singhal ,
E Einhorn ,
P Howlett ,
N A Cohen  and
N Mirza
A Ghosh*
Affiliation:
Department of Otorhinolaryngology: Head and Neck Surgery, University of Pennsylvania, Philadelphia
K P Leahy
Affiliation:
Department of Otorhinolaryngology: Head and Neck Surgery, University of Pennsylvania, Philadelphia
S Singhal
Affiliation:
Department of Thoracic Surgery, University of Pennsylvania, Philadelphia
E Einhorn
Affiliation:
Department of Pathology, Philadelphia Veterans’ Affairs Medical Center, Pennsylvania, USA
P Howlett
Affiliation:
Department of Pathology, Philadelphia Veterans’ Affairs Medical Center, Pennsylvania, USA
N A Cohen
Affiliation:
Department of Otorhinolaryngology: Head and Neck Surgery, University of Pennsylvania, Philadelphia
N Mirza
Affiliation:
Department of Otorhinolaryngology: Head and Neck Surgery, University of Pennsylvania, Philadelphia
*
Address for correspondence: Dr A Ghosh, Department of Otorhinolaryngology: Head and Neck Surgery, University of Pennsylvania, 3400 Spruce Street, 5 Ravdin, Philadelphia, PA 19104, USA E-mail: [email protected]
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Abstract

Objective:

This study aimed to develop a functional model of subglottic stenosis by inducing direct airway irritation in transplanted mouse laryngotracheal complexes.

Methods:

Laryngotracheal complexes from C57BL/6 mice were harvested and divided into three groups: uninjured, mechanically injured and chemically injured. Donor laryngotracheal complexes from each group were placed in dorsal subcutaneous pockets of recipient mice. Each week, the transplanted laryngotracheal complexes were harvested, and tissues were fixed, sectioned, and stained with haematoxylin and eosin. Representative slides were reviewed by a blinded pathologist, to determine the formation of granulation tissue, and graded as to the degree of granulation formation.

Results:

Direct airway irritation induced granulation tissue formation under the disrupted epithelium of airway mucosa; this was seen as early as two weeks after chemical injury.

Conclusion:

Results indicate that granulation tissue formation in a murine model may be an efficient tool for investigating the development and treatment of subglottic stenosis.

Keywords

Subglottic Stenosis, AcquiredAirway ObstructionGranulation TissueAnimal Model
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 130 , Issue 4 , April 2016 , pp. 380 - 387
Copyright
Copyright © JLO (1984) Limited 2016 

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References

1Hirshoren, N, Eliashar, R. Wound-healing modulation in upper airway stenosis - myths and facts. Head Neck 2009;21:111–26Google Scholar
2Nakagishi, Y, Morimoto, Y. Rabbit model of airway stenosis induced by scraping of the tracheal mucosa. Laryngoscope 2005;115:1087–92Google Scholar
3Roh, JL, Lee, YW, Park, HT. Subglottic wound healing in a new rabbit model of acquired subglottic stenosis. Ann Otol Rhinol Laryngol 2006;115:611–16CrossRefGoogle Scholar
4Branski, RC, Sandulache, VC, Dohar, JE, Hebda, PA. Mucosal wound healing in a rabbit model of subglottic stenosis: biochemical analysis of secretions. Arch Otolaryngol Head Neck Surg 2005;131:153–7Google Scholar
5Mitskavich, MT, Rimell, FL, Shapiro, AM. Porcine model of airway mucosal injury. Am J Otolaryngol 1997;18:315–19CrossRefGoogle ScholarPubMed
6Hertz, MI, Jessurun, J, King, MB, Savik, SK, Murray, JJ. Reproduction of the obliterative bronchiolitis lesion after heterotopic transplantation of mouse airways. Am J Pathol 1993;142:1945–51Google Scholar
7Kuo, E, Bharat, A. Role of airway epithelial injury in murine heterotopic tracheal allograft rejection. Ann Thorac Surg 2006;82:1226–33CrossRefGoogle ScholarPubMed
8Richter, GT, Mehta, D, Albert, D, Elluru, RG. A novel murine model for the examination of experimental subglottic stenosis. Arch Otolaryngol Head Neck Surg 2009;135:45–52Google Scholar
9Dohar, JE, Klein, EC, Betsch, JL, Hebda, PA. Acquired subglottic stenosis: depth and not extent of the insult is key. Int J Pediatr Otorhinolaryngol 1998;46:159–70CrossRefGoogle Scholar