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Microenvironment of macula flava in the human vocal fold as a stem cell niche

Published online by Cambridge University Press:  08 June 2016

K Sato ,
S Chitose ,
T Kurita  and
H Umeno
K Sato*
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Japan
S Chitose
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Japan
T Kurita
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Japan
H Umeno
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Japan
*
Address for correspondence: Dr Kiminori Sato, Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan Fax: +81 942 37 1200 E-mail: [email protected]
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Abstract

Background:

There is growing evidence that the cells in the maculae flavae are tissue stem cells of the human vocal fold mucosa, and that the maculae flavae are a candidate for a stem cell niche. The role of microenvironment in the maculae flavae of the human vocal fold mucosa was investigated.

Method:

Anterior maculae flavae from six surgical specimens were cultured in a mesenchymal stem cell growth medium or a Dulbecco's modified Eagle's medium.

Results:

Using mesenchymal stem cell growth medium, the subcultured cells formed a colony-forming unit, and cell division reflected asymmetric self-renewal. This indicates that these cells are mesenchymal stem cells or stromal stem cells in the bone marrow. Using Dulbecco's modified Eagle's medium, the subcultured cells showed symmetric cell division without a colony-forming unit.

Conclusion:

A proper microenvironment in the maculae flavae of the human vocal fold mucosa is necessary to be effective as a stem cell niche that maintains the stemness of the contained tissue stem cells.

Keywords

Vocal CordStem CellsStem Cell NicheLarynx
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 130 , Issue 7 , July 2016 , pp. 656 - 661
Copyright
Copyright © JLO (1984) Limited 2016 

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Footnotes

Presented at the 136th Annual Meeting of the American Laryngological Association, 22–23 April 2015, Boston, Massachusetts, USA.

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