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Increase in epithelial mast cell numbers in the nasal mucosa of patients with perennial allergic rhinitis

Published online by Cambridge University Press:  29 June 2007

A. Slater ,
L. A. Smallman  and
A. B. Drake-Lee
A. Slater*
Affiliation:
Department of Pathology, The Medical School, University of Birmingham, Birmingham, UK.
L. A. Smallman
Affiliation:
Department of Pathology, The Medical School, University of Birmingham, Birmingham, UK.
A. B. Drake-Lee
Affiliation:
Department of Otolaryngology, The Queen Elizabeth Hospital, Birmingham, UK.
*
Address for correspondence: Andrew Slater, 96 Warwards Lane, Selly Park, Birmingham B29 7RD.
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Abstract

The aim of the study was to compare the numbers and distribution of mast cells in the nasal mucosa of perennial allergic rhinitis (PAR) patients and controls, as demonstrated by different staining methods for light microscopy.

Biopsies of inferior turbinate mucosa were taken from 10 patients with PAR and 10 patients undergoing septoplasty or septorhinoplasty (control group). Sections for light microscopy were stained with azure A, chloroacetate esterase and an ABC immunohistochemical technique using antibody to tryptase.

Three times more mast cells were found in the epithelium of PAR patients compared to controls using the immunohistochemical technique (p = 0.0074). This method demonstrated considerably more mast cells than the other stains.

The increase in epithelial mast cells is consistent with the migration of mast cells seen in seasonal allergic rhinitis, and this may be important in the phenomenon of nasal priming seen after repeated antigen exposure.

Keywords

ImmunohistochemistryMast cells, Rhinitis, allergic, perennial
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 110 , Issue 10 , October 1996 , pp. 929 - 933
Copyright
Copyright © JLO (1984) Limited 1996

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Footnotes

This work has been presented at a meeting of the Midland Institute of Otolaryngology at City Hospital, Birmingham in January, 1994 and as a poster to the Winter Meeting of the Pathological Society of Great Britain and Ireland at the John Radcliffe Hospital, Oxford in January 1995.

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