Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-23T23:14:26.543Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunohistochemical evaluation with Ki-67: An application to salivary gland tumours

Published online by Cambridge University Press:  29 June 2007

M. Murakami ,
I. Ohtani ,
H. Hojo  and
H. Wakasa
M. Murakami*
Affiliation:
Department of Otolaryngology, Fukushima Medical College, Fukushima, Japan.
I. Ohtani
Affiliation:
Department of Otolaryngology, Fukushima Medical College, Fukushima, Japan.
H. Hojo
Affiliation:
Department of Pathology, Fukushima Medical College, Fukushima, Japan.
H. Wakasa
Affiliation:
Department of Pathology, Fukushima Medical College, Fukushima, Japan.
*
Dr M. Murakami, M.D., Matsukichyo 1–23, Fukushima City, Fukushima 960, Japan.
Get access Rights & Permissions [Opens in a new window]

Abstract

Using Ki-67, a monoclonal antibody, the proliferating capacity of 15 salivary gland tumours, including nine pleomorphic adenomas, four adenoid cystic carcinomas, one mucoepidermoid carcinoma and one acinic cell carcinoma was determined immunohistochemically, using normal salivary gland tissue as a control. The frequency of Ki-67 positive cells was 4.7 per cent in the normal salivary gland and one per cent in pleomorphic adenomas, whereas the average frequency in malignant tumours was 18.3 per cent. Among adenoid cystic carcinomas, the frequency was related to the morphological type; the solid sub-type had the highest frequency of Ki-67-positive cells. As this sub-type is recognized as the most aggressive of these tumours, this technique has the potential of providing an early indication of the clinical behaviour of a tumour.

Keywords

ImmunohistochemistrySalivary gland neoplasms
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 106 , Issue 1 , January 1992 , pp. 35 - 38
Copyright
Copyright © JLO (1984) Limited 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barnard, N. J., Hall, P. A., Lemoine, N. R., Kadar, N. (1987) Proliferative index in breast carcinoma determined in situ by Ki-67 immunostaining and its relationship to clinical and pathological variables. Journal of Pathology, 152: 287–295.Google Scholar
Gerdes, J., Schwab, U., Lemke, H., Stein, H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. International Journal of Cancer, 31: 13–20.CrossRefGoogle ScholarPubMed
Gerdes, J., Lemke, H., Baisch, H., Wacker, H.H., Schwab, U., Stein, H. (1984) Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. Journal of Immunology, 133: 1710–1715.Google Scholar
Grogan, T. M., Lippmann, S. M., Spier, C. M., Slymen, D. J., Rybski, J. A., Rangel, C. S., Richter, L. C., Miller, T. P. (1988) Independent prognostic significance of a nuclear proliferation antigen in difuse large cell lymphoma as determined by the monoclonal antibody Ki-67. Blood, 171: 1157–1160.Google Scholar
Hall, P. A., Richards, M. M., Gregory, W. M., d'Ardenne, A. J., Lister, T. A., Stansfeld, A. G. (1988) The prognostic value of Ki-67 immunostaining in non-Hodgkin's lymphoma. Journal of Pathology, 154: 223–235.CrossRefGoogle Scholar
Lorz, M., Meyer-Breiting, E. (1988) Evaluation of proliferative activity in human head and neck tumor using the monoclonal antibody Ki-67. Otorhinolaryngology, 50: 183–187.Google ScholarPubMed
Shibata, T., Burger, P. C., Kleihues, P. (1988) Cell kinetics of oligodendroglioma and oligo-astrocytoma. Ki-67 PAP study. No To Shinkei (Japan), 40: 779–785.Google ScholarPubMed
Sternberger, L. A., Hardy, P. H., Cuculis, J. J., Meyer, H. G. (1970) The unlabelled antibody enzyme method of immunochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase anti horseradish peroxidase) and its use in identification of spirochates. Journal of Histochemistry and Cytochemistry, 18: 315–333.CrossRefGoogle Scholar
Weiss, L. M., Strickler, J. G., Medeiros, L. J., Gerdes, J., Stein, H., Warnke, R. A. (1987) Proliferative rates of non-Hodgkin'slymphomas as assessed by Ki-67 antibody. Human Pathology, 18: 1155–1159.CrossRefGoogle ScholarPubMed