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GSTT1, GSTM1, GSTM3 and NAT2 polymorphisms in laryngeal squamous cell carcinoma in a Greek population

Published online by Cambridge University Press:  19 November 2009

M Chatzimichalis ,
J Xenellis ,
A Tzagaroulakis ,
P Sarof ,
K Banis ,
M Gazouli  and
A Bibas
M Chatzimichalis
Affiliation:
Department of Otolaryngology Head and Neck Surgery, Metaxa Anticancer Hospital, Piraeus, Greece
J Xenellis
Affiliation:
Department of Otolaryngology Head and Neck Surgery, School of Medicine, University of Athens, Greece
A Tzagaroulakis
Affiliation:
Department of Otolaryngology Head and Neck Surgery, School of Medicine, University of Athens, Greece
P Sarof
Affiliation:
Department of Otolaryngology Head and Neck Surgery, Metaxa Anticancer Hospital, Piraeus, Greece
K Banis
Affiliation:
Department of Otolaryngology Head and Neck Surgery, Metaxa Anticancer Hospital, Piraeus, Greece
M Gazouli*
Affiliation:
Department of Biology, School of Medicine, University of Athens, Greece
A Bibas
Affiliation:
Department of Otolaryngology Head and Neck Surgery, School of Medicine, University of Athens, Greece
*
Address for correspondence: Dr Maria Gazouli, Department of Biology, School of Medicine, University of Athens, Michalakopoulou 176, 11527 Athens, Greece. E-mail: [email protected]
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Abstract

Objective:

It is well known that laryngeal squamous cell carcinoma is strongly related to tobacco and alcohol consumption. Accumulating evidence suggests that alterations of detoxification enzymes, such as glutathione S-transferases and N-acetyltransferases, influence the risk of cancers associated with tobacco smoke and alcohol.

Methods:

This was a retrospective case–control study. The study group consisted of 88 Greek patients with laryngeal squamous cell carcinoma; there were also 102 control subjects. Frequencies of the genotypes GSTT1, GSTM1, GSTM3 and NAT2 were evaluated by polymerase chain reaction restriction fragment polymorphism.

Results:

The distribution of overall genotypes was 55.68 per cent rapid acetylator and 44.32 per cent slow acetylator in patients, and 36.27 per cent rapid acetylator and 63.72 per cent slow acetylator in controls. The odds ratio for rapid acetylator status in cases versus controls was 2.207 (95 per cent confidence interval 1.23–3.95, p = 0.0087).

Conclusion:

This study demonstrated a significant relationship between rapid acetylator genotypes and laryngeal squamous cell carcinoma in a Greek population.

Keywords

Laryngeal Squamous Cell CarcinomaGene PolymorphismsGSTT1GSTM1GSTM3NAT2
Type
Main Article
Information
The Journal of Laryngology & Otology , Volume 124 , Issue 3 , March 2010 , pp. 318 - 323
Copyright
Copyright © JLO (1984) Limited 2009

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