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Expression of maspin in invasive fungal rhinosinusitis

Published online by Cambridge University Press:  29 December 2016

Y D Huang ,
H W Yu ,
S W Xia ,
Z H Kang ,
Y S He  and
D Y Han
Y D Huang
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, PLA 118 Hospital, Wenzhou, Zhejiang Province
H W Yu
Affiliation:
ENT Department, Huzhou Central Hospital, Zhejiang Province
S W Xia
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, PLA 118 Hospital, Wenzhou, Zhejiang Province
Z H Kang
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, PLA 118 Hospital, Wenzhou, Zhejiang Province
Y S He
Affiliation:
ENT Department, Huzhou Central Hospital, Zhejiang Province
D Y Han*
Affiliation:
ENT Institute, PLA General Hospital, Beijing, China
*
Address for correspondence: Dr Dong Yi Han, ENT Institute, PLA General Hospital, Beijing 100039, China Fax: +86 10 6693 6193 E-mail: [email protected]
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Abstract

Objective:

This study aimed to test the expression of maspin in invasive fungal rhinosinusitis and explore its value in diagnosing invasive fungal rhinosinusitis.

Methods:

Forty-two fungal rhinosinusitis cases (12 invasive and 30 non-invasive) were selected as the experimental group, and 30 chronic rhinosinusitis cases comprised the control group. Maspin expression was assessed in nasal mucous membrane specimens by immunohistochemical staining.

Results:

Compared with the control group, maspin expression was down-regulated in the fungal rhinosinusitis group (p < 0.05). Furthermore, the staining score for maspin was lowest in the invasive fungal rhinosinusitis group, as compared with both the non-invasive fungal rhinosinusitis group and the control group (p < 0.05). A maspin staining score of 5.70 was the critical value for diagnosis of invasive fungal rhinosinusitis, with sensitivity and specificity of 91.7 per cent and 88.3 per cent, respectively.

Conclusion:

The results of this study suggest that the maspin staining score may be a biomarker for effective and rapid diagnosis of invasive fungal rhinosinusitis.

Keywords

ImmunohistochemistryMycosesSinusitisInvasive Fungal InfectionMaspin
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 131 , Issue 2 , February 2017 , pp. 150 - 154
Copyright
Copyright © JLO (1984) Limited 2016 

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References

1 Granville, L, Chirala, M, Cernoch, P, Ostrowski, M, Truong, LD. Fungal sinusitis: histologic spectrum and correlation with culture. Hum Pathol 2004;35:474–81CrossRefGoogle ScholarPubMed
2 Hora, JF. Primary aspergillosis of the paranasal sinuses and associated areas. Laryngoscope 1965;75:768–73CrossRefGoogle ScholarPubMed
3 deShazo, RD, O'Brien, M, Chapin, K, Soto-Aguilar, M, Gardner, L, Swain, R. A new classification and diagnostic criteria for invasive fungal sinusitis. Arch Otolaryngol Head Neck Surg 1997;123:1181–8CrossRefGoogle ScholarPubMed
4 Ribes, JA, Vanover-Sams, CL, Baker, DJ. Zygomycetes in human disease. Clin Microbiol Rev 2000;13:236–301 CrossRefGoogle ScholarPubMed
5 Knutsen, AP, Hutchinson, PS, Albers, GM, Consolino, J, Smick, J, Kurup, VP. Increased sensitivity to IL-4 in cystic fibrosis patients with allergic bronchopulmonary aspergillosis. Allergy 2004;59:81–7CrossRefGoogle ScholarPubMed
6 Ayala, P, Vasquez, B, Wetzler, L, So, M. Neisseria gonorrhoeae porin P1.B induces endosome exocytosis and a redistribution of Lamp1 to the plasma membrane. Infect Immun 2002;70:5965–71CrossRefGoogle Scholar
7 Sheng, S, Carey, J, Seftor, EA, Dias, L, Hendrix, MJ, Sager, R. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc Natl Acad Sci U S A 1996;93:11669–74CrossRefGoogle ScholarPubMed
8 Umekita, Y, Ohi, Y, Sagara, Y, Yoshida, H. Expression of maspin predicts poor prognosis in breast-cancer patients. Int J Cancer 2002;100:452–5CrossRefGoogle ScholarPubMed
9 Beecken, WD, Engl, T, Engels, K, Blumenberg, C, Oppermann, E, Camphausen, K et al. Clinical relevance of maspin expression in bladder cancer. World J Urol 2006;24:338–44CrossRefGoogle ScholarPubMed
10 Kim, K, Sohn, H, Kim, JS, Choi, HG, Byun, EH, Lee, KI et al. Mycobacterium tuberculosis Rv0652 stimulates production of tumour necrosis factor and monocytes chemoattractant protein-1 in macrophages through the Toll-like receptor 4 pathway. Immunology 2012;136:231–40CrossRefGoogle ScholarPubMed
11 Soler, ZM, Schlosser, RJ. The role of fungi in diseases of the nose and sinuses. Am J Rhinol Allergy 2012;26:351–8CrossRefGoogle ScholarPubMed
12 Zou, Z, Anisowicz, A, Hendrix, MJ, Thor, A, Neveu, M, Sheng, S et al. Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells. Science 1994;28:526–9CrossRefGoogle Scholar
13 Maass, N, Teffner, M, Rösel, F, Pawaresch, R, Jonat, W, Nagasaki, K et al. Decline in the expression of the serine proteinase inhibitor maspin is associated with tumour progression in ductal carcinomas of the breast. J Pathol 2001;195:321–6CrossRefGoogle ScholarPubMed
14 Odero-Marah, VA, Khalkhali-Ellis, Z, Chunthapong, J, Amir, S, Seftor, RE, Seftor, EA et al. Maspin regulates different signaling pathways for motility and adhesion in aggressive breast cancer cells. Cancer Biol Ther 2003;2:398–403 CrossRefGoogle ScholarPubMed
15 Lin, YT, Verma, A, Hodgkinson, CP. Toll-like receptors and human disease: lessons from single nucleotide polymorphisms. Curr Genomics 2012;13:633–45CrossRefGoogle ScholarPubMed
16 Wuthrich, M, Deepe, GS Jr, Klein, B. Adaptive immunity to fungi. Annu Rev Immunol 2012;30:115–48CrossRefGoogle ScholarPubMed
17 Newton, K, Dixit, VM. Signaling in innate immunity and inflammation. Cold Spring Harb Perspect Biol 2012;4 CrossRefGoogle ScholarPubMed
18 Kim, ST, Choi, JH, Jeon, HG, Cha, HE, Hwang, YJ, Chung, YS. Comparison between polymerase chain reaction and fungal culture for the detection of fungi in patients with chronic sinusitis and normal controls. Acta Otolaryngol 2005;125:72–5CrossRefGoogle ScholarPubMed
19 Zhang, F, An, YF, Zhao, CQ. Preliminary study on Aspergillus fumigatus-induced IKKα controlled maspin protein expression in respiratory epithelial cells of rats [in Chinese]. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2013;48:48–53 Google Scholar