Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2025-01-05T13:10:38.918Z Has data issue: false hasContentIssue false
  • English
  • Français

Expression of EGF, EGFR and PCNA in laryngeal lesions

Published online by Cambridge University Press:  29 June 2007

Q. H. Wen ,
T. Nishimura ,
T. Miwa ,
I. Nagayama  and
M. Furukawa
Q. H. Wen*
Affiliation:
Department of Otolaryngology, School of Medicine, Kanazawa University, Kanazawa, Japan.
T. Nishimura
Affiliation:
Department of Otolaryngology, School of Medicine, Kanazawa University, Kanazawa, Japan.
T. Miwa
Affiliation:
Department of Otolaryngology, School of Medicine, Kanazawa University, Kanazawa, Japan.
I. Nagayama
Affiliation:
Department of Otolaryngology, School of Medicine, Kanazawa University, Kanazawa, Japan.
M. Furukawa
Affiliation:
Department of Otolaryngology, School of Medicine, Kanazawa University, Kanazawa, Japan.
*
Dr Q. H. Wen, Department of Otolaryngology, School of Medicine, Kanazawa University, Kanazawa, Japan. Fax: 00-81-762-344265
Get access Rights & Permissions [Opens in a new window]

Abstract

The expression of EGF/EGFR in 47 laryngeal surgical specimens from 44 patients was examined. PCNA analysis as an index of proliferating cells was also performed in 32 cases of laryngeal cancer, six cases of pre-cancerous lesions and nine cases of normal laryngeal mucosa. EGFR failed to show a significant correlation with tumour behaviour, but EGF expression was statistically significantly higher in malignant (SCC) than in non-malignant tissues (pre-cancerous and normal tissues) (p<0.006), and PCNA also showed a statistically significant difference (p<0.016) between the two. In malignant tissues when EGF/EGFR in ‘double-positive’ and ‘double-negative’ cases was compared, a statistically significant difference in PCNA was found (p<0.029); but this was not seen in non-malignant tissues. Our results support the hypothesis that an autocrine mechanism exists in laryngeal cancer and in this mechanism EGF may play an important role in tumour progression, especially when EGFR is overexpressed.

Keywords

Laryngeal neoplasmsImmunohistochemistry
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 109 , Issue 7 , July 1995 , pp. 630 - 636
Copyright
Copyright © JLO (1984) Limited 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adamson, E. D., Rees, A. R. (1981) Epidermal growth factor receptors. Molecular and Cellular Biochemistry 34: 129–152.Google Scholar
Bravo, R., Celis, J. E. (1980) A search for differential polypeptide synthesis throughout the cell cycle of HeLa cells. Journal of Cell Biology 84: 795–802.Google Scholar
Bravo, R., MacDonald-Bravo, H. (1984) Induction of the nuclear protein cyclin in quiescent mouse 3T3 cells stimulated by serum and growth factors: Correlation with DNA synthesis. EMBO Journal 3: 3177–3181.CrossRefGoogle ScholarPubMed
Bravo, R., MacDonald-Bravo, H. (1985) Changes in the nuclear distribution of cyclin (PCNA) but not its synthesis depend on DNA replication. EMBO Journal 4: 655–661.Google Scholar
Bujia, J, Wustrow, T. P. U. (1993) Heterologous epidermal growth factor receptor (EGF-R) expression in larynx cancer cell lines: evidence for the existence of structurally modified receptor. Acta Oto-laryngologica (Stockholm) 113: 789–792.Google Scholar
Celis, J. E., Bravo, R., Larsen, P. M., Fey, S. J. (1984) Cyclin: a nuclear protein whose level correlates directly with the proliferative state of normal and transformed cells. Leukemia Research 8: 143–157.CrossRefGoogle ScholarPubMed
Celis, J. E., Celis, A. (1985) Cell cycle dependent variations in the distribution of the nuclear protein cyclin proliferating cell nuclear antigen in cultured subdivision of S phase. Proceedings of the National Academy of Sciences of the United States of America 82: 3262–3266.Google Scholar
Cowley, G., Smith, J. A., Gusterson, B. A. (1986) Increased EGF receptors on human squamous carcinoma cell lines. British Journal of Cancer 53: 223–229.CrossRefGoogle ScholarPubMed
Derynck, R., Roberts, A. B., Winkler, M. E., Chen, E. Y., Goeddel, D. V. (1984) Human transforming growth factoralpha: precursor structure and expression in E. coli. Cell 38: 287–297.Google Scholar
Downward, J., Yarden, Y., Mayes, E., Scrace, G., Totty, N., Stockwell, P., Viirich, A., Schlessinger, J., Waterfield, M. D. (1984) Close similarity of epidermal growth factor receptor with v-erb-B oncogene protein sequences. Nature (London) 307: 521–527.Google Scholar
Galand, P., Degraef, C. (1989) Cyclin/PCNA immunostaining as an alternative to tritiated thymidine pulse labelling for marking S-phase cell in paraffin sections from aminol and human tissues. Cell and Tissue Kinetics 22: 383–392.Google Scholar
Garcia, R. L., Coltrera, M. D., Gown, A. M. (1989) Analysis of proliferative grade using anti-PCNA-cyclin monoclonal antibodies in fixed, embedded tissues. Comparison with flow cytometric analysis. American Journal of Pathology 134: 733–739.Google Scholar
Gill, G., Kawamoto, T., Cochet, C., Le, A. D, Mendelsohn, J., Polikoff, J., Sato, G. H. (1984) Monoclonal anti-epidermal growth factor receptor antibodies which are inhibitors of epidermal growth factor binding and antagonists of epidermal growth factor-stimulated tyrosine protein kinase activity. Journal of Biological Chemistry 259: 7755–7760.CrossRefGoogle ScholarPubMed
Hall, P. A., Levison, D. A., Woods, A. L., Yu, C. C.-W., Kellock, D. B., Watkins, J. A., Barnes, D. M. (1990) Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. Journal of Pathology 162: 285–294.CrossRefGoogle ScholarPubMed
Iihara, K., Shiozaki, H., Tahala, H., Kobayashi, K., Inoue, M., Tamura, S. (1993) Prognostic significance of transforming growth factor-α in human esophageal carcinoma. Cancer 71: 2902–2909.3.0.CO;2-J>CrossRefGoogle ScholarPubMed
Ishitoya, J., Toriyama, M., Oguchi, N., Kitamura, K., Ohshima, M., Asano, K., Yamamoto, T. (1989) Gene amplification and overexpression of EGF receptor in squamous cell carcinomas of the head and neck. British Journal of Cancer 59: 559–562.CrossRefGoogle ScholarPubMed
Jaskulski, D., Gatti, G., Travalli, S., Calabretta, B., Baserga, R. (1988) Regulation of the proliferating cell nuclear antigen cyclin and thymidine kinase mRNA levels by growth factors. Journal of Biological Chemistry 263: 10175–10179.CrossRefGoogle ScholarPubMed
Kawamoto, T., Takahashi, K., Nishi, M., Kimura, T. (1991) Quantitative assay of epidermal growth factor receptor in human squamous cell carcinomas of the oral region by an avidin-biotin method. Japanese Journal of Cancer Research 82: 403–410.CrossRefGoogle ScholarPubMed
Miyachi, K., Fritzler, M. J., Tarn, E. M. (1978) Autoantibody to a nuclear antigen in proliferating cell. Journal of Immunology 121: 2228–2234.Google Scholar
Miyaguchi, M, Olofsson, J., Hellquist, H. B. (1990) Expression of epidermal growth factor receptor in laryngeal dysplasia and carcinoma. Ada Oto-laryngologica (Stockholm) 110: 309–313.CrossRefGoogle ScholarPubMed
Miyaguchi, M., Olofsson, J., Hellquist, H. B. (1991a) Expression of epidermal growth factor receptor in glottic carcinoma and its relation to recurrence after radiotherapy. Clinical Otolaryngology 16: 466–469.CrossRefGoogle ScholarPubMed
Miyaguchi, M., Olofsson, J., Hellquist, H. B. (1991b) Immunohistochemical study of epidermal growth factor receptor in severe dysplasia and carcinoma in situ of the vocal cords. Acta Oto-laryngologica (Stockholm) 111: 142–152.Google Scholar
Ozanne, B., Richards, C. S., Hendler, E., Burns, D., Gusterson, B. (1986) Over expression of the EGF receptor is a hallmark of squamous cell carcinomas. Journal of Pathology 149: 9–14.Google Scholar
Ozawa, S., Ueda, M., Ando, N., Shimizu, N., Abe, O. (1989) Prognostic significance of epidermal growth factor receptor in esophageal squamous cell carcinomas. Cancer 63: 2169–2173.Google Scholar
Partridge, M., Gullick, W. J. (1988) Expression of epidermal growth factor receptor on oral squamous cell carcinoma. British Journal of Oral and Maxillofacial Surgery 26: 381–389.CrossRefGoogle ScholarPubMed
Sainsbury, J. R. C., Farndon, J. R. (1987) Epidermal growth factor receptor status as predictor of early recurrence of and death from breast cancer. Lancet i: 1398–1402.Google Scholar
Santon, J. B., Cronin, M. T., Macleod, C. L. (1986) Effects of epidermal growth factor receptor concentration on tumorigenicity of A431 cells in nude mice. Cancer Research 46: 4701–4705.Google ScholarPubMed
Smetana, K., Gyorkey, F., Chan, P.-K, Tan, E.-M., Busch, H. (1983) Proliferating cell nuclear antigen (PCNA) and human malignant nucleolar antigens (HMTNA) in nucleoli of human haematological malignancies. Blutalkohol 46: 133–141.Google Scholar
Smith, K., Fennelly, J. A., Neal, D. E., Hall, R. R., Harris, A. L. (1989) Characterization and quantitation of the epidermal growth factor receptor in invasive and superficial bladder tumours. Cancer Research 49: 5810–5815.Google Scholar
Sporn, M. B., Todaro, G. J. (1980) Autocrine secretion and malignant transformation of cells. New England Journal of Medicine 303: 878–880.Google Scholar
Sporn, M. B., Roberts, A. B. (1985) Autocrine growth factors and cancer. Nature 313: 28.Google Scholar
Ullrich, A., Coussens, L., Hayflick, T. S. (1985) Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. Nature 309: 418–425.Google Scholar
Yasui, W., Sumiyoshi, H., Hata, J., Kameda, T., Ochiai, A., Ito, H., Tahara, E. (1988) Epidermal growth factor receptor in human gastric and colonic carcinomas. Cancer Research 48: 137–141.Google Scholar