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Evaluation of caspase-3 and caspase-8 deregulation in tongue squamous cell carcinoma, based on immunohistochemistry and computerised image analysis

Published online by Cambridge University Press:  23 May 2008

D Andressakis ,
A C Lazaris ,
E Tsiambas ,
N Kavantzas ,
A Rapidis  and
E Patsouris
D Andressakis
Affiliation:
Department of Oral and Maxillofacial Surgery, St Savas Anticancer Hospital, Athens, Greece
A C Lazaris
Affiliation:
Department of Pathology, Medical School, National & Kapodistrian University, Athens, Greece
E Tsiambas*
Affiliation:
Department of Immunohistochemistry, 401GA Hospital, Athens, Greece
N Kavantzas
Affiliation:
Department of Pathology, Medical School, National & Kapodistrian University, Athens, Greece
A Rapidis
Affiliation:
Department of Oral and Maxillofacial Surgery, St Savas Anticancer Hospital, Athens, Greece
E Patsouris
Affiliation:
Department of Pathology, Medical School, National & Kapodistrian University, Athens, Greece
*
Address for correspondence: Dr E Tsiambas, SOA No 20, Vathi-Samos, Greece83100. Fax: 00302273027947, E-mail: [email protected]
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Abstract

Aims:

To investigate the potential role of caspase-3 and caspase-8 protein expression in the biological behaviour of tongue squamous cell carcinoma.

Materials and methods:

We conducted immunohistochemical analyses of 87 specimens of primary tongue squamous cell carcinoma, using monoclonal anti-caspase-3 and anti-caspase-8 antibodies. A digital image analysis assay was also performed in order to evaluate the results.

Results:

Reduced expression of caspase-8 and -3 proteins was observed in 30/87 (34.5 per cent) and 79/87 (90.5 per cent) cases, respectively. Cox regression analysis showed no prognostic significance for the association between overall protein expression of either marker and survival probability (p = 0.174 for caspase-3; p = 0.608 for caspase-8). Interestingly, the size of the examined tumours was strongly correlated with survival status (p = 0.024).

Conclusions:

Simultaneous deregulation of caspase-8 and -3 is a frequent event in tongue squamous cell carcinoma. Activation of caspase-3, which is predominantly down-regulated, may be a crucial process for induction of apoptosis and response to therapeutic strategies.

Keywords

CaspasesApoptosisTongueSquamous Cell CarcinomaImage Analysis
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 122 , Issue 11 , November 2008 , pp. 1213 - 1218
Copyright
Copyright © JLO (1984) Limited 2008

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