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Epstein-Barr virus BHRF1 prohibits the cells of nasopharyngeal carcinoma from apoptosis

Published online by Cambridge University Press:  29 June 2007

H. Huang ,
J. H. Zhou ,
S. M. Zhou ,
J. H. Hu ,
X. H. Pan ,
X. T. Kong ,
L. Yu ,
X. Y. Sun  and
W. Wu
H. Huang*
Affiliation:
Department of Otolaryngology, Changhai Hospital, Institute of Genetics, Shanghai 200433, People's Republic of China.
J. H. Zhou
Affiliation:
Department of Pharmacology, Changhai Hospital, Institute of Genetics, Shanghai 200433, People's Republic of China.
S. M. Zhou
Affiliation:
Department of Otolaryngology, Changhai Hospital, Institute of Genetics, Shanghai 200433, People's Republic of China.
J. H. Hu
Affiliation:
Department of Pharmacology, Changhai Hospital, Institute of Genetics, Shanghai 200433, People's Republic of China.
X. H. Pan
Affiliation:
Department of Biology, The Second Military Medical University, Shanghai 200433, People's Republic of China.
X. T. Kong
Affiliation:
Department of Central Laboratory of Clinical Immunology, General Military, Changzheng Hospital, The Second Military Medical University, Shanghai 200433, People's Republic of China.
L. Yu
Affiliation:
Department of Central Laboratory of Clinical Immunology, General Military, Changzheng Hospital, The Second Military Medical University, Shanghai 200433, People's Republic of China.
W. Wu
Affiliation:
Department of Otolaryngology, Changhai Hospital, Institute of Genetics, Shanghai 200433, People's Republic of China.
*
Address for correspondence: Hai Huang, Department of Otolaryngology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, People's Republic of China.
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Abstract

Epstein-Barr virus (EBV) is associated with nasopharyngeal carcinoma (NPC). The BHRF1 EBV protein is expressed at high levels in productively infected cells and certain latently infected cells. In order to investigate the effect of expression of BHRF1 on the biological behaviour of NPC cells, we constructed the BHRF1 high expression vector and transfected it into the NPC cell line, CNE2. Then, the alteration of proliferation and apoptotic rates in the cells were tested before and after camptothecin treatment. After treatment by camptothecin, BHRF1-CNE2 cells could constantly and slowly proliferate and its apoptotic rate was less than in control groups, and the number of cells in the G phase decreased and in the S phase increased. So, it suggests that BHRF1 expression can enhance the resistibility of CNE2 cells to DNA damaging agents that cause apoptosis.

Keywords

Epstein-Barr virusNasopharyngeal neoplasmsCarcinomaCamptothecinGenesviral
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 111 , Issue 12 , December 1997 , pp. 1147 - 1150
Copyright
Copyright © JLO (1984) Limited 1997

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